(Circular) (Six-base) MAPSORT of: pFN21AB9096.seq Check: 815 from: 1 to: 6511 pFN21AB9096 6511 bp With 182 enzymes: SgfI * June 11, 2012 14:10 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4321 278 Size: 53 83 186 936 2785 2468 Fragments arranged by size: 2785 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3854 1937 Size: 1917 4594 Acc65I G'GTAC_C Cuts at: 3832 3832 Size: 6511 AclI AA'CG_TT Cuts at: 1563 4638 5011 1563 Size: 3075 373 3063 Fragments arranged by size: 3075 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2564 2831 3573 3646 4574 5958 6226 1632 Size: 932 267 742 73 928 1384 268 1917 Fragments arranged by size: 1917 1384 932 928 742 268 267 73 AfeI AGC'GCT Cuts at: 3101 3101 Size: 6511 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6314 AflIII A'CryG_T Cuts at: 1203 2439 3258 5410 6387 1203 Size: 1236 819 2152 977 1327 Fragments arranged by size: 2152 1327 1236 977 819 AgeI A'CCGG_T Cuts at: 5342 5342 Size: 6511 AhdI GACnn_n'nnGTC Cuts at: 5240 5240 Size: 6511 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6511 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6511 AlwNI CAG_nnn'CTG Cuts at: 2143 2857 4254 5826 2143 Size: 714 1397 1572 2828 Fragments arranged by size: 2828 1572 1397 714 ApaI G_GGCC'C Cuts at: 2873 2873 Size: 6511 ApaLI G'TGCA_C Cuts at: 4568 5724 4568 Size: 1156 5355 ApoI r'AATT_y Cuts at: 1493 3820 4046 4198 6307 1493 Size: 2327 226 152 2109 1697 Fragments arranged by size: 2327 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5065 160 Size: 872 4033 1606 Fragments arranged by size: 4033 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6511 AvaI C'yCGr_G Cuts at: 1870 1943 2578 3112 3836 1870 Size: 73 635 534 724 4545 Fragments arranged by size: 4545 724 635 534 73 BamHI G'GATC_C Cuts at: 3792 3841 4208 3792 Size: 49 367 6095 Fragments arranged by size: 6095 367 49 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2184 3152 3323 3832 5287 Size: 325 217 627 397 968 171 509 1455 Cuts at: 5287 618 Size: 1842 Fragments arranged by size: 1842 1455 968 627 509 397 325 217 171 BanII G_rGCy'C Cuts at: 1391 2747 2873 3036 3783 3830 1391 Size: 1356 126 163 747 47 4072 Fragments arranged by size: 4072 1356 747 163 126 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2262 2779 3430 928 Size: 971 363 517 651 4009 Fragments arranged by size: 4009 971 651 517 363 BbvCI CC'TCA_GC Cuts at: 2060 2060 Size: 6511 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4702 4736 1903 Size: 34 2765 34 3678 Fragments arranged by size: 3678 2765 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3076 4403 5613 3076 Size: 1327 1210 3974 Fragments arranged by size: 3974 1327 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6511 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5122 136 Size: 107 122 71 631 605 3450 1525 Fragments arranged by size: 3450 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6506 6506 Size: 6511 BlpI GC'TnA_GC Cuts at: 3035 3122 3928 3035 Size: 87 806 5618 Fragments arranged by size: 5618 806 87 Bme1580I G_kGCm'C Cuts at: 1790 2187 2873 3155 4572 5728 1790 Size: 397 686 282 1417 1156 2573 Fragments arranged by size: 2573 1417 1156 686 397 282 BmgBI CAC'GTC Cuts at: 1318 2440 2832 1318 Size: 1122 392 4997 Fragments arranged by size: 4997 1122 392 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2764 2875 3315 Size: 416 53 83 399 24 1347 111 440 Cuts at: 3315 3377 4242 5200 442 Size: 62 865 958 1753 Fragments arranged by size: 1753 1347 958 865 440 416 399 111 83 62 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6511 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2000 2846 3654 5171 1239 Size: 761 846 808 1517 2579 Fragments arranged by size: 2579 1517 846 808 761 Bpu10I CC'TnA_GC Cuts at: 2060 2615 2060 Size: 555 5956 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4636 5501 5799 4636 Size: 865 298 5348 Fragments arranged by size: 5348 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5174 882 Size: 617 3675 2219 Fragments arranged by size: 3675 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2326 3259 3652 4302 493 Size: 711 1122 933 393 650 2702 Fragments arranged by size: 2702 1122 933 711 650 393 BsaBI GATnn'nnATC Cuts at: 2159 3164 6505 2159 Size: 1005 3341 2165 Fragments arranged by size: 3341 2165 1005 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3758 4318 4700 5335 Size: 53 83 186 936 2225 560 382 635 Cuts at: 5335 275 Size: 1451 Fragments arranged by size: 2225 1451 936 635 560 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2488 4944 5342 5616 5763 6174 2488 Size: 2456 398 274 147 411 2825 Fragments arranged by size: 2825 2456 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2238 2268 2546 2576 2750 Size: 30 165 30 835 30 278 30 174 Cuts at: 2750 2780 1178 Size: 30 4909 Fragments arranged by size: 4909 835 278 174 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2354 2570 2573 3515 3689 1186 Size: 1168 216 3 942 174 4008 Fragments arranged by size: 4008 1168 942 216 174 3 BseYI C'CCAG_C Cuts at: 2382 2403 2894 3181 3418 5714 6210 2382 Size: 21 491 287 237 2296 496 2683 Fragments arranged by size: 2683 2296 496 491 287 237 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2804 3110 1163 Size: 573 1068 306 4564 Fragments arranged by size: 4564 1068 573 306 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3108 3961 4722 5750 1540 Size: 275 186 1107 853 761 1028 2301 Fragments arranged by size: 2301 1107 1028 853 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2171 3123 3551 4572 4657 5728 2171 Size: 952 428 1021 85 1071 2954 Fragments arranged by size: 2954 1071 1021 952 428 85 BsmI GAATG_Cn' Cuts at: 3340 4022 4115 3340 Size: 682 93 5736 Fragments arranged by size: 5736 682 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2822 3115 2822 Size: 293 6218 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2171 2187 2747 2873 3036 3123 3155 Size: 399 381 16 560 126 163 87 32 Cuts at: 3155 3551 3783 3830 4572 4657 5728 1391 Size: 396 232 47 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 560 399 396 381 232 163 126 87 85 47 32 16 BspEI T'CCGG_A Cuts at: 6174 6174 Size: 6511 BspHI T'CATG_A Cuts at: 2904 4400 6130 2904 Size: 1496 1730 3285 Fragments arranged by size: 3285 1730 1496 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2828 3866 844 Size: 1984 1038 3489 Fragments arranged by size: 3489 1984 1038 BsrBI CCG'CTC Cuts at: 1394 1823 4398 1394 Size: 429 2575 3507 Fragments arranged by size: 3507 2575 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3213 5006 5180 65 Size: 1183 1965 1793 174 1396 Fragments arranged by size: 1965 1793 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3238 3522 5155 5342 1700 Size: 110 1428 284 1633 187 2869 Fragments arranged by size: 2869 1633 1428 284 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6511 BssHII G'CGCG_C Cuts at: 1925 2349 1925 Size: 424 6087 BssSI C'ACGA_G Cuts at: 4571 5583 4571 Size: 1012 5499 BstAPI GCAn_nnn'nTGC Cuts at: 794 2413 3452 794 Size: 1619 1039 3853 Fragments arranged by size: 3853 1619 1039 BstBI TT'CG_AA Cuts at: 5315 5315 Size: 6511 BstEII G'GTnAC_C Cuts at: 1184 2212 1184 Size: 1028 5483 BstXI CCAn_nnnn'nTGG Cuts at: 3772 3772 Size: 6511 BstYI r'GATC_y Cuts at: 1972 2971 3792 3841 4208 4593 4610 6062 6148 Size: 999 821 49 367 385 17 1452 86 Cuts at: 6148 6160 6506 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 999 821 385 367 346 86 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2038 3736 513 Size: 552 939 34 1698 3288 Fragments arranged by size: 3288 1698 939 552 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5772 Fragments arranged by size: 5772 683 56 BtsI GCAGTG_nn' Cuts at: 3549 4027 4821 4841 3549 Size: 478 794 20 5219 Fragments arranged by size: 5219 794 478 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6511 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6476 DraI TTT'AAA Cuts at: 3815 3815 Size: 6511 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5518 809 Size: 507 4202 1802 Fragments arranged by size: 4202 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2700 3003 3105 Size: 54 1425 50 275 18 870 303 102 Cuts at: 3105 3958 4847 6476 8 Size: 853 889 1629 43 Fragments arranged by size: 1629 1425 889 870 853 303 275 102 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3105 3958 1537 Size: 275 1293 853 4090 Fragments arranged by size: 4090 1293 853 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2396 2426 4441 1353 Size: 1043 30 2015 3423 Fragments arranged by size: 3423 2015 1043 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2737 3303 3722 5098 5472 5618 Size: 1064 138 1148 566 419 1376 374 146 Cuts at: 5618 387 Size: 1280 Fragments arranged by size: 1376 1280 1148 1064 566 419 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2000 2564 2831 2846 3573 3646 3654 Size: 393 368 564 267 15 727 73 8 Cuts at: 3654 4574 5171 5958 6226 1239 Size: 920 597 787 268 1524 Fragments arranged by size: 1524 920 787 727 597 564 393 368 268 267 73 15 8 EcoNI CCTnn'n_nnAGG Cuts at: 2135 2192 2135 Size: 57 6454 EcoO109I rG'GnC_Cy Cuts at: 2431 2869 2870 2431 Size: 438 1 6072 Fragments arranged by size: 6072 438 1 EcoRI G'AATT_C Cuts at: 3820 4198 3820 Size: 378 6133 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3334 3334 Size: 6511 FspI TGC'GCA Cuts at: 5017 5017 Size: 6511 HaeII r_GCGC'y Cuts at: 1678 2390 3103 5658 1678 Size: 712 713 2555 2531 Fragments arranged by size: 2555 2531 713 712 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2086 2118 2192 2224 2546 Size: 32 135 32 514 32 74 32 322 Cuts at: 2546 2578 5152 5184 5226 5258 1373 Size: 32 2574 32 42 32 2626 Fragments arranged by size: 2626 2574 514 322 135 74 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2451 2692 3855 4101 669 Size: 988 281 513 241 1163 246 3079 Fragments arranged by size: 3079 1163 988 513 281 246 241 HindIII A'AGCT_T Cuts at: 748 3488 748 Size: 2740 3771 HpaI GTT'AAC Cuts at: 4101 4101 Size: 6511 KpnI G_GTAC'C Cuts at: 3836 3836 Size: 6511 MfeI C'AATT_G Cuts at: 4110 4110 Size: 6511 MluI A'CGCG_T Cuts at: 6387 6387 Size: 6511 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5625 5809 1305 Size: 4320 184 2007 Fragments arranged by size: 4320 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2702 3005 10 Size: 54 1425 343 870 303 3516 Fragments arranged by size: 3516 1425 870 343 303 54 MslI CAynn'nnrTG Cuts at: 518 1166 2288 2903 3770 4469 4828 4987 518 Size: 648 1122 615 867 699 359 159 2042 Fragments arranged by size: 2042 1122 867 699 648 615 359 159 MspA1I CmG'CkG Cuts at: 1606 2344 3181 3329 3724 3927 4604 5752 5997 Size: 738 837 148 395 203 677 1148 245 Cuts at: 5997 1606 Size: 2120 Fragments arranged by size: 2120 1148 837 738 677 395 245 203 148 NaeI GCC'GGC Cuts at: 3524 3524 Size: 6511 NcoI C'CATG_G Cuts at: 513 1065 2004 2038 513 Size: 552 939 34 4986 Fragments arranged by size: 4986 939 552 34 NdeI CA'TA_TG Cuts at: 387 387 Size: 6511 NgoMIV G'CCGG_C Cuts at: 3522 3522 Size: 6511 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6511 NotI GC'GGCC_GC Cuts at: 3958 3958 Size: 6511 NspI r_CATG'y Cuts at: 1132 1229 2280 2902 3427 3869 5414 1132 Size: 97 1051 622 525 442 1545 2229 Fragments arranged by size: 2229 1545 1051 622 525 442 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6511 PciI A'CATG_T Cuts at: 5410 5410 Size: 6511 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6511 PfoI T'CCnGG_A Cuts at: 2016 2479 2016 Size: 463 6048 PmeI GTTT'AAAC Cuts at: 3815 3815 Size: 6511 PmlI CAC'GTG Cuts at: 2326 3259 3652 2326 Size: 933 393 5185 Fragments arranged by size: 5185 933 393 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4580 4612 6093 6125 4580 Size: 32 1481 32 4966 Fragments arranged by size: 4966 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2431 2431 Size: 6511 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6511 PsiI TTA'TAA Cuts at: 4081 4081 Size: 6511 PspOMI G'GGCC_C Cuts at: 2869 2869 Size: 6511 PstI C_TGCA'G Cuts at: 830 2343 2862 3499 3863 830 Size: 1513 519 637 364 3478 Fragments arranged by size: 3478 1513 637 519 364 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6511 PvuII CAG'CTG Cuts at: 3181 3724 3181 Size: 543 5968 SalI G'TCGA_C Cuts at: 1936 3853 1936 Size: 1917 4594 SapI GCTCTTCn'nnn_ Cuts at: 2396 2426 2396 Size: 30 6481 SbfI CC_TGCA'GG Cuts at: 2862 3863 2862 Size: 1001 5510 ScaI AGT'ACT Cuts at: 4759 6471 4759 Size: 1712 4799 SfcI C'TryA_G Cuts at: 826 1045 2253 2339 2858 3495 3597 3859 4994 Size: 219 1208 86 519 637 102 262 1135 Cuts at: 4994 5675 5866 826 Size: 681 191 1471 Fragments arranged by size: 1471 1208 1135 681 637 519 262 219 191 102 86 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6511 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6511 SmaI CCC'GGG Cuts at: 1872 2580 3838 1872 Size: 708 1258 4545 Fragments arranged by size: 4545 1258 708 SmlI C'TyrA_G Cuts at: 820 1017 1943 4615 5516 5778 820 Size: 197 926 2672 901 262 1553 Fragments arranged by size: 2672 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4302 493 Size: 3809 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6511 SphI G_CATG'C Cuts at: 1132 2902 3427 3869 1132 Size: 1770 525 442 3774 Fragments arranged by size: 3774 1770 525 442 SspI AAT'ATT Cuts at: 5 52 2522 4435 5 Size: 47 2470 1913 2081 Fragments arranged by size: 2470 2081 1913 47 StuI AGG'CCT Cuts at: 1517 3317 1517 Size: 1800 4711 StyI C'CwwG_G Cuts at: 513 1065 2004 2038 3027 3950 513 Size: 552 939 34 989 923 3074 Fragments arranged by size: 3074 989 939 923 552 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2417 4538 4555 4708 4893 5338 325 Size: 1255 837 2121 17 153 185 445 1498 Fragments arranged by size: 2121 1498 1255 837 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4757 6469 96 Size: 274 80 33 51 1443 2780 1712 138 Fragments arranged by size: 2780 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2475 2507 3357 3389 6471 6503 2475 Size: 32 850 32 3082 32 2483 Fragments arranged by size: 3082 2483 850 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6511 XbaI T'CTAG_A Cuts at: 3847 3847 Size: 6511 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2666 2710 3772 2666 Size: 44 1062 5405 Fragments arranged by size: 5405 1062 44 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6511 XmaI C'CCGG_G Cuts at: 1870 2578 3836 1870 Size: 708 1258 4545 Fragments arranged by size: 4545 1258 708 XmnI GAAnn'nnTTC Cuts at: 4640 4640 Size: 6511 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4319 276 Size: 53 83 186 936 2785 2468 Fragments arranged by size: 2785 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI