Restriction map for
pFN21AB9096
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbeI BcgI
BclI BfrI BglI BglII BlnI
BsaI BseAI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrGI BssSI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI HpaI KasI KpnI
KspI MfeI MluI MroI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsaI RsrII
SacI SacII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SspBI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 1752 GGCGTC G[GA]^CG[CT]C
Aor51HI 1094 AGCGCT AGC^GCT
ApaI 864 GGGCCC GGGCC^C
BamHI 1787 GGATCC G^GATCC
BbvCI 54 CCTCAGC CCTCAGC(-5/-2)
BciVI 1077 GGATAC GTATCC(6/5)
BfaI 1792 CTAG C^TAG
BsaHI 1752 GGCGTC G[GA]^CG[CT]C
BsaWI 483 TCCGGT [AT]^CCGG[AT]
BsiEI 1100 CGGCCG CG[GA][CT]^CG
BsmI 1329 GAATGC GAATGC(1/-1)
BspHI 899 TCATGA T^CATGA
BspMI 832 GCAGGT ACCTGC(4/8)
BsrDI 1209 CATTGC GCAATG(2/0)
BssHII 344 GCGCGC G^CGCGC
BstEII 207 GGTGACC G^GT[ACGT]ACC
BstPI 207 GGTGACC G^GT[ACGT]ACC
BstXI 1760 CCACACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 1100 CGGCCG C^GGCCG
EclXI 1100 CGGCCG C^GGCCG
Eco47III 1094 AGCGCT AGC^GCT
Eco52I 1100 CGGCCG C^GGCCG
EcoO65I 207 GGTGACC G^GT[ACGT]ACC
HgaI 1753 GCGTC GACGC(5/10)
Hin1I 1752 GGCGTC G[GA]^CG[CT]C
HindIII 1483 AAGCTT A^AGCTT
MaeI 1792 CTAG C^TAG
NaeI 1517 GCCGGC GCC^GGC
NcoI 33 CCATGG C^CATGG
NgoMIV 1517 GCCGGC G^CCGGC
PpuMI 425 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 899 TCATGA T^CATGA
SbfI 852 CCTGCAGG CCTGCA^GG
SmaI 573 CCCGGG CCC^GGG
Sse8387I 852 CCTGCAGG CCTGCA^GG
SspI 515 AATATT AAT^ATT
StuI 1310 AGGCCT AGG^CCT
XmaCI 573 CCCGGG C^CCGGG
XmaI 573 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 216 CGCG CG^CG
345 CGCG
AflIII 434 ACGTGT A^C[GA][CT]GT
1253 ACGTGT
AlwNI 133 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
847 CAGTGCCTG
AvaI 573 CCCGGG C^[CT]CG[GA]G
1107 CTCGGG
BalI 695 TGGCCA TGG^CCA
998 TGGCCA
BlpI 1029 GCTCAGC GC^T[ACGT]AGC
1116 GCTCAGC
Bpu1102I 1029 GCTCAGC GC^T[ACGT]AGC
1116 GCTCAGC
BsaBI 150 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1155 GATAAGCATC
BsgI 814 CTGCAC GTGCAG(16/14)
1084 GTGCAG
BsmBI 1104 CGTCTC CGTCTC(1/5)
823 GAGACG
BsoBI 573 CCCGGG C^[CT]CG[GA]G
1107 CTCGGG
BsrFI 1233 GCCGGT [GA]^CCGG[CT]
1517 GCCGGC
BstAPI 402 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1441 GCAACTGCTGC
BstUI 216 CGCG CG^CG
345 CGCG
BstYI 966 GGATCT [GA]^GATC[CT]
1787 GGATCC
CelII 1029 GCTCAGC GC^T[ACGT]AGC
1116 GCTCAGC
Cfr10I 1233 GCCGGT [GA]^CCGG[CT]
1517 GCCGGC
DsaI 33 CCATGG C^C[GA][CT]GG
1731 CCGTGG
EarI 385 CTCTTC CTCTTC(1/4)
415 CTCTTC
EcoNI 126 CCTGTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
183 CCTGGGCAAGG
EcoT14I 33 CCATGG C^C[AT][AT]GG
1022 CCTTGG
FauI 176 GCGGG CCCGC(4/6)
339 GCGGG
HaeII 381 GGCGCT [GA]GCGC^[CT]
1094 AGCGCT
HincII 444 GTTGAC GT[CT]^[GA]AC
685 GTCAAC
HindII 444 GTTGAC GT[CT]^[GA]AC
685 GTCAAC
Ksp632I 385 CTCTTC CTCTTC(1/4)
415 CTCTTC
MamI 150 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1155 GATAAGCATC
MflI 966 GGATCT [GA]^GATC[CT]
1787 GGATCC
MluNI 695 TGGCCA TGG^CCA
998 TGGCCA
MscI 695 TGGCCA TGG^CCA
998 TGGCCA
MvnI 216 CGCG CG^CG
345 CGCG
PvuII 1174 CAGCTG CAG^CTG
1717 CAGCTG
SapI 384 GCTCTTC GCTCTTC(1/4)
414 GCTCTTC
SphI 893 GCATGC GCATG^C
1418 GCATGC
StyI 33 CCATGG C^C[AT][AT]GG
1022 CCTTGG
TfiI 1017 GATTC G^A[AT]TC
1397 GAATC
XhoII 966 GGATCT [GA]^GATC[CT]
1787 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanI BanII BbrPI BbsI BbvI
BcnI BmyI BpmI BpuAI BsaAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PmaCI PmlI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp45I TspRI XcmI