Restriction map for
pFN21AB9096
(Linear) (Six-base) MAPSORT of: KIBB9096.seq Check: 2718 from: 1 to: 1806
KIBB9096.orf
With 182 enzymes: SgfI *
June 11, 2012 13:41 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 826 1568 1641 1806
Size: 559 267 742 73 165
Fragments arranged by size:
742 559 267 165 73
AfeI AGC'GCT
Cuts at: 0 1096 1806
Size: 1096 710
AflIII A'CryG_T
Cuts at: 0 434 1253 1806
Size: 434 819 553
Fragments arranged by size:
819 553 434
AlwNI CAG_nnn'CTG
Cuts at: 0 138 852 1806
Size: 138 714 954
Fragments arranged by size:
954 714 138
ApaI G_GGCC'C
Cuts at: 0 868 1806
Size: 868 938
AvaI C'yCGr_G
Cuts at: 0 573 1107 1806
Size: 573 534 699
Fragments arranged by size:
699 573 534
BamHI G'GATC_C
Cuts at: 0 1787 1806
Size: 1787 19
BanI G'GyrC_C
Cuts at: 0 179 1147 1318 1806
Size: 179 968 171 488
Fragments arranged by size:
968 488 179 171
BanII G_rGCy'C
Cuts at: 0 742 868 1031 1778 1806
Size: 742 126 163 747 28
Fragments arranged by size:
747 742 163 126 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 257 774 1425 1806
Size: 257 517 651 381
Fragments arranged by size:
651 517 381 257
BbvCI CC'TCA_GC
Cuts at: 0 55 1806
Size: 55 1751
BciVI GTATCCnnnnn_n'
Cuts at: 0 1071 1806
Size: 1071 735
BlpI GC'TnA_GC
Cuts at: 0 1030 1117 1806
Size: 1030 87 689
Fragments arranged by size:
1030 689 87
Bme1580I G_kGCm'C
Cuts at: 0 182 868 1150 1806
Size: 182 686 282 656
Fragments arranged by size:
686 656 282 182
BmgBI CAC'GTC
Cuts at: 0 435 827 1806
Size: 435 392 979
Fragments arranged by size:
979 435 392
BmrI ACTGGGnnnn_n'
Cuts at: 0 759 870 1310 1372 1806
Size: 759 111 440 62 434
Fragments arranged by size:
759 440 434 111 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 841 1649 1806
Size: 841 808 157
Fragments arranged by size:
841 808 157
Bpu10I CC'TnA_GC
Cuts at: 0 55 610 1806
Size: 55 555 1196
Fragments arranged by size:
1196 555 55
BsaAI yAC'GTr
Cuts at: 0 321 1254 1647 1806
Size: 321 933 393 159
Fragments arranged by size:
933 393 321 159
BsaBI GATnn'nnATC
Cuts at: 0 154 1159 1806
Size: 154 1005 647
Fragments arranged by size:
1005 647 154
BsaHI Gr'CG_yC
Cuts at: 0 1753 1806
Size: 1753 53
BsaWI w'CCGG_w
Cuts at: 0 483 1806
Size: 483 1323
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 233 263 541 571 745 775 1806
Size: 233 30 278 30 174 30 1031
Fragments arranged by size:
1031 278 233 174 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 349 565 568 1510 1684 1806
Size: 349 216 3 942 174 122
Fragments arranged by size:
942 349 216 174 122 3
BseYI C'CCAG_C
Cuts at: 0 377 398 889 1176 1413 1806
Size: 377 21 491 287 237 393
Fragments arranged by size:
491 393 377 287 237 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1105 1806
Size: 799 306 701
Fragments arranged by size:
799 701 306
BsiEI CG_ry'CG
Cuts at: 0 1103 1806
Size: 1103 703
BsiHKAI G_wGCw'C
Cuts at: 0 166 1118 1546 1806
Size: 166 952 428 260
Fragments arranged by size:
952 428 260 166
BsmI GAATG_Cn'
Cuts at: 0 1335 1806
Size: 1335 471
BsmBI CGTCTCn'nnnn_
Cuts at: 0 817 1110 1806
Size: 817 293 696
Fragments arranged by size:
817 696 293
Bsp1286I G_dGCh'C
Cuts at: 0 166 182 742 868 1031 1118 1150 1546
Size: 166 16 560 126 163 87 32 396
Cuts at: 1546 1778 1806
Size: 232 28
Fragments arranged by size:
560 396 232 166 163 126 87 32
28 16
BspHI T'CATG_A
Cuts at: 0 899 1806
Size: 899 907
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 823 1806
Size: 823 983
BsrDI GCAATG_nn'
Cuts at: 0 1208 1806
Size: 1208 598
BsrFI r'CCGG_y
Cuts at: 0 1233 1517 1806
Size: 1233 284 289
Fragments arranged by size:
1233 289 284
BssHII G'CGCG_C
Cuts at: 0 344 1806
Size: 344 1462
BstAPI GCAn_nnn'nTGC
Cuts at: 0 408 1447 1806
Size: 408 1039 359
Fragments arranged by size:
1039 408 359
BstEII G'GTnAC_C
Cuts at: 0 207 1806
Size: 207 1599
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1767 1806
Size: 1767 39
BstYI r'GATC_y
Cuts at: 0 966 1787 1806
Size: 966 821 19
Fragments arranged by size:
966 821 19
BtgI C'CryG_G
Cuts at: 0 33 1731 1806
Size: 33 1698 75
Fragments arranged by size:
1698 75 33
BtsI GCAGTG_nn'
Cuts at: 0 1544 1806
Size: 1544 262
EaeI y'GGCC_r
Cuts at: 0 695 998 1100 1806
Size: 695 303 102 706
Fragments arranged by size:
706 695 303 102
EagI C'GGCC_G
Cuts at: 0 1100 1806
Size: 1100 706
EarI CTCTTCn'nnn_
Cuts at: 0 391 421 1806
Size: 391 30 1385
Fragments arranged by size:
1385 391 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 732 1298 1717 1806
Size: 732 566 419 89
Fragments arranged by size:
732 566 419 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 826 841 1568 1641 1649 1806
Size: 559 267 15 727 73 8 157
Fragments arranged by size:
727 559 267 157 73 15 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 130 187 1806
Size: 130 57 1619
Fragments arranged by size:
1619 130 57
EcoO109I rG'GnC_Cy
Cuts at: 0 426 864 865 1806
Size: 426 438 1 941
Fragments arranged by size:
941 438 426 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1329 1806
Size: 1329 477
HaeII r_GCGC'y
Cuts at: 0 385 1098 1806
Size: 385 713 708
Fragments arranged by size:
713 708 385
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 81 113 187 219 541 573 1806
Size: 81 32 74 32 322 32 1233
Fragments arranged by size:
1233 322 81 74 32 32 32
HincII GTy'rAC
Cuts at: 0 446 687 1806
Size: 446 241 1119
Fragments arranged by size:
1119 446 241
HindIII A'AGCT_T
Cuts at: 0 1483 1806
Size: 1483 323
MscI TGG'CCA
Cuts at: 0 697 1000 1806
Size: 697 303 806
Fragments arranged by size:
806 697 303
MslI CAynn'nnrTG
Cuts at: 0 283 898 1765 1806
Size: 283 615 867 41
Fragments arranged by size:
867 615 283 41
MspA1I CmG'CkG
Cuts at: 0 339 1176 1324 1719 1806
Size: 339 837 148 395 87
Fragments arranged by size:
837 395 339 148 87
NaeI GCC'GGC
Cuts at: 0 1519 1806
Size: 1519 287
NcoI C'CATG_G
Cuts at: 0 33 1806
Size: 33 1773
NgoMIV G'CCGG_C
Cuts at: 0 1517 1806
Size: 1517 289
NspI r_CATG'y
Cuts at: 0 275 897 1422 1806
Size: 275 622 525 384
Fragments arranged by size:
622 525 384 275
PfoI T'CCnGG_A
Cuts at: 0 11 474 1806
Size: 11 463 1332
Fragments arranged by size:
1332 463 11
PmlI CAC'GTG
Cuts at: 0 321 1254 1647 1806
Size: 321 933 393 159
Fragments arranged by size:
933 393 321 159
PpuMI rG'GwC_Cy
Cuts at: 0 426 1806
Size: 426 1380
PspOMI G'GGCC_C
Cuts at: 0 864 1806
Size: 864 942
PstI C_TGCA'G
Cuts at: 0 338 857 1494 1806
Size: 338 519 637 312
Fragments arranged by size:
637 519 338 312
PvuII CAG'CTG
Cuts at: 0 1176 1719 1806
Size: 1176 543 87
Fragments arranged by size:
1176 543 87
SapI GCTCTTCn'nnn_
Cuts at: 0 391 421 1806
Size: 391 30 1385
Fragments arranged by size:
1385 391 30
SbfI CC_TGCA'GG
Cuts at: 0 857 1806
Size: 857 949
SfcI C'TryA_G
Cuts at: 0 248 334 853 1490 1592 1806
Size: 248 86 519 637 102 214
Fragments arranged by size:
637 519 248 214 102 86
SmaI CCC'GGG
Cuts at: 0 575 1806
Size: 575 1231
SphI G_CATG'C
Cuts at: 0 897 1422 1806
Size: 897 525 384
Fragments arranged by size:
897 525 384
SspI AAT'ATT
Cuts at: 0 517 1806
Size: 517 1289
StuI AGG'CCT
Cuts at: 0 1312 1806
Size: 1312 494
StyI C'CwwG_G
Cuts at: 0 33 1022 1806
Size: 33 989 784
Fragments arranged by size:
989 784 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 412 1806
Size: 412 1394
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 470 502 1352 1384 1806
Size: 470 32 850 32 422
Fragments arranged by size:
850 470 422 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 661 705 1767 1806
Size: 661 44 1062 39
Fragments arranged by size:
1062 661 44 39
XmaI C'CCGG_G
Cuts at: 0 573 1806
Size: 573 1233
Enzymes that do cut:
AcuI AfeI AflIII AlwNI ApaI AvaI BamHI BanI
BanII BbsI BbvCI BciVI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrFI BssHII BstAPI BstEII BstXI BstYI
BtgI BtsI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII HindIII MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PfoI PmlI PpuMI
PspOMI PstI PvuII SapI SbfI SfcI SmaI SphI
SspI StuI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BcgI BclI BfrBI BglI BglII
BmtI BplI BpuEI BsaI BsiWI BspEI BsrBI BsrGI
BssSI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EcoICRI EcoRI EcoRV FseI FspI FspAI
HpaI KasI KpnI MfeI MluI MmeI NarI NdeI
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SrfI SwaI TatI
Tth111I XbaI XhoI XmnI ZraI