RNF149 ORF clone pFN21AB8710

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Halotag ORF Clone pFN21AB8710

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Clone info.

Species

human

Clone Name

pFN21AB8710 Sequence (with vector) Restriction Map

Product ID

FHC02955

Flexi Clone PID

FXC02955

ORF Sequence

DNA sequence (1200 bp)
Protein sequence (400 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463234]

Gene

KIBB8710

Symbol

RNF149

Alias : DNAPTP2

Description

ring finger protein 149

Original Clone ID

oc00373

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1200 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 400 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2051.1 5.9e-121 99.8 RNF149 CCDS34065.1 7.7e-39 43.5 RNF150 CCDS64340.1 1.9e-35 41.7 RNF130 CCDS4451.1 2e-35 41.7 RNF130 CCDS14521.1 7.3e-35 41.3 RNF128 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003137 110 173 PF02225 Protease-associated domain IPR018957 269 309 PF00097 Zinc finger HMMSmart IPR001841 269 309 SM00184 Zinc finger ProfileScan IPR001841 269 310 PS50089 Zinc finger
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 10 VGARGVLALALLALALCVPGAR 31 PRIMARY 22 2 152 HAGTGNIVVIMISYPKGREILEL 174 SECONDARY 23 3 199 QSVVFVAIAFITMMIISLAWLIF 221 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_919445 428 300439 41.3 87.5 NP_078815 402 300439 39.6 82.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8710 Download

>KIBB8710  1200 bp
ATGGCGTGGCGGCGGCGCGAAGCCAGCGTCGGGGCTCGCGGCGTGTTGGCTCTGGCGTTG
CTCGCCCTGGCCCTGTGCGTGCCCGGGGCCCGGGGCCGGGCTCTCGAGTGGTTCTCGGCC
GTGGTAAACATCGAGTACGTGGACCCGCAGACCAACCTGACGGTGTGGAGCGTCTCGGAG
AGTGGCCGCTTCGGCGACAGCTCGCCCAAGGAGGGCGCGCATGGCCTGGTGGGCGTCCCG
TGGGCGCCCGGCGGAGACCTCGAGGGCTGCGCGCCCGACACGCGCTTCTTCGTGCCCGAG
CCCGGCGGCCGAGGGGCCGCGCCCTGGGTCGCCCTGGTGGCTCGTGGGGGCTGCACCTTC
AAGGACAAGGTGCTGGTGGCGGCGCGGAGGAACGCCTCGGCCGTCGTCCTCTACAATGAG
GAGCGCTACGGGAACATCACCTTGCCCATGTCTCACGCGGGAACAGGAAATATAGTGGTC
ATTATGATTAGCTATCCAAAAGGAAGAGAAATTTTGGAGCTGGTGCAAAAAGGAATTCCA
GTAACGATGACCATAGGGGTTGGCACCCGGCATGTACAGGAGTTCATCAGCGGTCAGTCT
GTGGTGTTTGTGGCCATTGCCTTCATCACCATGATGATTATCTCGTTAGCCTGGCTAATA
TTTTACTATATACAGCGTTTCCTATATACTGGCTCTCAGATTGGAAGTCAGAGCCATAGA
AAAGAAACTAAGAAAGTTATTGGCCAGCTTCTACTTCATACTGTAAAGCATGGAGAAAAG
GGAATTGATGTTGATGCTGAAAATTGTGCAGTGTGTATTGAAAATTTCAAAGTAAAGGAT
ATTATTAGAATTCTGCCATGCAAGCATATTTTTCATAGAATATGCATTGACCCATGGCTT
TTGGATCACCGAACATGTCCAATGTGTAAACTTGATGTCATCAAAGCCCTAGGATATTGG
GGAGAGCCTGGGGATGTACAGGAGATGCCTGCTCCAGAATCTCCTCCTGGAAGGGATCCA
GCTGCAAATTTGAGTCTAGCTTTACCAGATGATGACGGAAGTGATGAGAGCAGTCCACCA
TCAGCCTCCCCTGCTGAATCTGAGCCACAGTGTGATCCCAGCTTTAAAGGAGATGCAGGA
GAAAATACGGCATTGCTAGAAGCCGGCAGGAGTGACTCTCGGCATGGAGGACCCATCTCC

ORF Protein

Cloned ORF protein sequence for pFN21AB8710 Download

>KIBB8710  400 aa
MAWRRREASVGARGVLALALLALALCVPGARGRALEWFSAVVNIEYVDPQTNLTVWSVSE
SGRFGDSSPKEGAHGLVGVPWAPGGDLEGCAPDTRFFVPEPGGRGAAPWVALVARGGCTF
KDKVLVAARRNASAVVLYNEERYGNITLPMSHAGTGNIVVIMISYPKGREILELVQKGIP
VTMTIGVGTRHVQEFISGQSVVFVAIAFITMMIISLAWLIFYYIQRFLYTGSQIGSQSHR
KETKKVIGQLLLHTVKHGEKGIDVDAENCAVCIENFKVKDIIRILPCKHIFHRICIDPWL
LDHRTCPMCKLDVIKALGYWGEPGDVQEMPAPESPPGRDPAANLSLALPDDDGSDESSPP
SASPAESEPQCDPSFKGDAGENTALLEAGRSDSRHGGPIS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8710 Download

>pFN21AB8710  5905 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGTGGCGGCGGCGCGAAGCCAGCGTCGGGGC
TCGCGGCGTGTTGGCTCTGGCGTTGCTCGCCCTGGCCCTGTGCGTGCCCGGGGCCCGGGG
CCGGGCTCTCGAGTGGTTCTCGGCCGTGGTAAACATCGAGTACGTGGACCCGCAGACCAA
CCTGACGGTGTGGAGCGTCTCGGAGAGTGGCCGCTTCGGCGACAGCTCGCCCAAGGAGGG
CGCGCATGGCCTGGTGGGCGTCCCGTGGGCGCCCGGCGGAGACCTCGAGGGCTGCGCGCC
CGACACGCGCTTCTTCGTGCCCGAGCCCGGCGGCCGAGGGGCCGCGCCCTGGGTCGCCCT
GGTGGCTCGTGGGGGCTGCACCTTCAAGGACAAGGTGCTGGTGGCGGCGCGGAGGAACGC
CTCGGCCGTCGTCCTCTACAATGAGGAGCGCTACGGGAACATCACCTTGCCCATGTCTCA
CGCGGGAACAGGAAATATAGTGGTCATTATGATTAGCTATCCAAAAGGAAGAGAAATTTT
GGAGCTGGTGCAAAAAGGAATTCCAGTAACGATGACCATAGGGGTTGGCACCCGGCATGT
ACAGGAGTTCATCAGCGGTCAGTCTGTGGTGTTTGTGGCCATTGCCTTCATCACCATGAT
GATTATCTCGTTAGCCTGGCTAATATTTTACTATATACAGCGTTTCCTATATACTGGCTC
TCAGATTGGAAGTCAGAGCCATAGAAAAGAAACTAAGAAAGTTATTGGCCAGCTTCTACT
TCATACTGTAAAGCATGGAGAAAAGGGAATTGATGTTGATGCTGAAAATTGTGCAGTGTG
TATTGAAAATTTCAAAGTAAAGGATATTATTAGAATTCTGCCATGCAAGCATATTTTTCA
TAGAATATGCATTGACCCATGGCTTTTGGATCACCGAACATGTCCAATGTGTAAACTTGA
TGTCATCAAAGCCCTAGGATATTGGGGAGAGCCTGGGGATGTACAGGAGATGCCTGCTCC
AGAATCTCCTCCTGGAAGGGATCCAGCTGCAAATTTGAGTCTAGCTTTACCAGATGATGA
CGGAAGTGATGAGAGCAGTCCACCATCAGCCTCCCCTGCTGAATCTGAGCCACAGTGTGA
TCCCAGCTTTAAAGGAGATGCAGGAGAAAATACGGCATTGCTAGAAGCCGGCAGGAGTGA
CTCTCGGCATGGCGGACCCATCTCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT