Restriction map for
pFN21AB8710
(Linear) (Six-base) MAPSORT of: KIBB8710.seq Check: 1322 from: 1 to: 1200
KIBB8710.orf
With 182 enzymes: SgfI *
February 20, 2009 16:37 ..
AfeI AGC'GCT
Cuts at: 0 424 1200
Size: 424 776
AflIII A'CryG_T
Cuts at: 0 913 1200
Size: 913 287
ApaI G_GGCC'C
Cuts at: 0 90 1200
Size: 90 1110
ApoI r'AATT_y
Cuts at: 0 509 533 822 848 1026 1200
Size: 509 24 289 26 178 174
Fragments arranged by size:
509 289 178 174 26 24
AvaI C'yCGr_G
Cuts at: 0 82 89 103 259 295 1200
Size: 82 7 14 156 36 905
Fragments arranged by size:
905 156 82 36 14 7
AvrII C'CTAG_G
Cuts at: 0 948 1200
Size: 948 252
BamHI G'GATC_C
Cuts at: 0 1014 1200
Size: 1014 186
BanI G'GyrC_C
Cuts at: 0 243 562 1200
Size: 243 319 638
Fragments arranged by size:
638 319 243
BanII G_rGCy'C
Cuts at: 0 36 90 102 302 1200
Size: 36 54 12 200 898
Fragments arranged by size:
898 200 54 36 12
BbeI G_GCGC'C
Cuts at: 0 247 1200
Size: 247 953
BfrBI ATG'CAT
Cuts at: 0 884 1200
Size: 884 316
BglI GCCn_nnn'nGGC
Cuts at: 0 191 230 337 1200
Size: 191 39 107 863
Fragments arranged by size:
863 191 107 39
Bme1580I G_kGCm'C
Cuts at: 0 83 90 296 1200
Size: 83 7 206 904
Fragments arranged by size:
904 206 83 7
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 129 1193 1200
Size: 129 1064 7
Fragments arranged by size:
1064 129 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 1200
Size: 977 223
BsaI GGTCTCn'nnnn_
Cuts at: 0 248 1200
Size: 248 952
BsaAI yAC'GTr
Cuts at: 0 138 1200
Size: 138 1062
BsaBI GATnn'nnATC
Cuts at: 0 637 1200
Size: 637 563
BsaHI Gr'CG_yC
Cuts at: 0 233 244 1200
Size: 233 11 956
Fragments arranged by size:
956 233 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 433 992 1200
Size: 433 559 208
Fragments arranged by size:
559 433 208
BseYI C'CCAG_C
Cuts at: 0 1117 1200
Size: 1117 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 827 1200
Size: 336 491 373
Fragments arranged by size:
491 373 336
BsiEI CG_ry'CG
Cuts at: 0 119 309 401 1200
Size: 119 190 92 799
Fragments arranged by size:
799 190 119 92
BsmBI CGTCTCn'nnnn_
Cuts at: 0 177 1200
Size: 177 1023
Bsp1286I G_dGCh'C
Cuts at: 0 36 83 90 102 296 302 1200
Size: 36 47 7 12 194 6 898
Fragments arranged by size:
898 194 47 36 12 7 6
BsrDI GCAATG_nn'
Cuts at: 0 614 1150 1200
Size: 614 536 50
Fragments arranged by size:
614 536 50
BsrFI r'CCGG_y
Cuts at: 0 1162 1200
Size: 1162 38
BsrGI T'GTAC_A
Cuts at: 0 573 975 1200
Size: 573 402 225
Fragments arranged by size:
573 402 225
BssHII G'CGCG_C
Cuts at: 0 215 269 1200
Size: 215 54 931
Fragments arranged by size:
931 215 54
BssSI C'ACGA_G
Cuts at: 0 341 1200
Size: 341 859
BstYI r'GATC_y
Cuts at: 0 1014 1200
Size: 1014 186
BtgI C'CryG_G
Cuts at: 0 119 238 892 1200
Size: 119 119 654 308
Fragments arranged by size:
654 308 119 119
BtsI GCAGTG_nn'
Cuts at: 0 815 1200
Size: 815 385
DraI TTT'AAA
Cuts at: 0 1125 1200
Size: 1125 75
DraIII CAC_nnn'GTG
Cuts at: 0 1111 1200
Size: 1111 89
EaeI y'GGCC_r
Cuts at: 0 116 183 306 398 611 741 1200
Size: 116 67 123 92 213 130 459
Fragments arranged by size:
459 213 130 123 116 92 67
EagI C'GGCC_G
Cuts at: 0 116 306 398 1200
Size: 116 190 92 802
Fragments arranged by size:
802 190 116 92
EarI CTCTTCn'nnn_
Cuts at: 0 498 1200
Size: 498 702
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 266 1200
Size: 266 934
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 1200
Size: 977 223
EcoO109I rG'GnC_Cy
Cuts at: 0 86 1189 1200
Size: 86 1103 11
Fragments arranged by size:
1103 86 11
EcoRI G'AATT_C
Cuts at: 0 533 848 1200
Size: 533 315 352
Fragments arranged by size:
533 352 315
HaeII r_GCGC'y
Cuts at: 0 247 426 1200
Size: 247 179 774
Fragments arranged by size:
774 247 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 477 509 690 722 975 1007 1178 1200
Size: 477 32 181 32 253 32 171 22
Fragments arranged by size:
477 253 181 171 32 32 32 22
KasI G'GCGC_C
Cuts at: 0 243 1200
Size: 243 957
MscI TGG'CCA
Cuts at: 0 613 743 1200
Size: 613 130 457
Fragments arranged by size:
613 457 130
MslI CAynn'nnrTG
Cuts at: 0 628 631 1200
Size: 628 3 569
Fragments arranged by size:
628 569 3
MspA1I CmG'CkG
Cuts at: 0 590 1021 1200
Size: 590 431 179
Fragments arranged by size:
590 431 179
NaeI GCC'GGC
Cuts at: 0 1164 1200
Size: 1164 36
NarI GG'CG_CC
Cuts at: 0 244 1200
Size: 244 956
NcoI C'CATG_G
Cuts at: 0 892 1200
Size: 892 308
NgoMIV G'CCGG_C
Cuts at: 0 1162 1200
Size: 1162 38
NsiI A_TGCA'T
Cuts at: 0 886 1200
Size: 886 314
NspI r_CATG'y
Cuts at: 0 574 917 1200
Size: 574 343 283
Fragments arranged by size:
574 343 283
PasI CC'CwG_GG
Cuts at: 0 323 1200
Size: 323 877
PciI A'CATG_T
Cuts at: 0 913 1200
Size: 913 287
PfoI T'CCnGG_A
Cuts at: 0 1005 1200
Size: 1005 195
PpuMI rG'GwC_Cy
Cuts at: 0 1189 1200
Size: 1189 11
PspOMI G'GGCC_C
Cuts at: 0 86 1200
Size: 86 1114
PspXI vC'TCGA_Gb
Cuts at: 0 259 1200
Size: 259 941
PvuII CAG'CTG
Cuts at: 0 1021 1200
Size: 1021 179
SfoI GGC'GCC
Cuts at: 0 245 1200
Size: 245 955
SmaI CCC'GGG
Cuts at: 0 84 91 1200
Size: 84 7 1109
Fragments arranged by size:
1109 84 7
SmlI C'TyrA_G
Cuts at: 0 103 259 1200
Size: 103 156 941
Fragments arranged by size:
941 156 103
SspI AAT'ATT
Cuts at: 0 659 1200
Size: 659 541
StyI C'CwwG_G
Cuts at: 0 206 892 948 1200
Size: 206 686 56 252
Fragments arranged by size:
686 252 206 56
TatI w'GTAC_w
Cuts at: 0 573 975 1200
Size: 573 402 225
Fragments arranged by size:
573 402 225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 458 490 1200
Size: 458 32 710
Fragments arranged by size:
710 458 32
XhoI C'TCGA_G
Cuts at: 0 103 259 1200
Size: 103 156 941
Fragments arranged by size:
941 156 103
XmaI C'CCGG_G
Cuts at: 0 82 89 1200
Size: 82 7 1111
Fragments arranged by size:
1111 82 7
Enzymes that do cut:
AfeI AflIII ApaI ApoI AvaI AvrII BamHI BanI
BanII BbeI BfrBI BglI Bme1580I BplI BpmI BsaI
BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiEI BsmBI
Bsp1286I BsrDI BsrFI BsrGI BssHII BssSI BstYI BtgI
BtsI DraI DraIII EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI HaeII Hin4I KasI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PfoI PpuMI PspOMI PspXI PvuII SfoI SmaI SmlI
SspI StyI TatI TstI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI BaeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaWI BsaXI
BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrBI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DrdI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI
NheI NotI NruI PacI PflMI PmeI PmlI PpiI
PshAI PsiI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI
TaqII Tth111I XbaI XcmI XmnI ZraI