Restriction map for
pFN21AB8710
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlpI
Bpu1102I BpuAI BsaWI BseAI BsiHKAI
BsiWI BsmI BspDI BspEI BspHI
BspMI BsrBI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DrdI Eam1105I
Eco81I EcoNI EcoO65I EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AflIII 913 ACATGT A^C[GA][CT]GT
Aor51HI 422 AGCGCT AGC^GCT
ApaI 86 GGGCCC GGGCC^C
AvrII 948 CCTAGG C^CTAGG
BamHI 1014 GGATCC G^GATCC
BbeI 243 GGCGCC GGCGC^C
BlnI 948 CCTAGG C^CTAGG
BpmI 992 CTCCAG CTGGAG(16/14)
BsaAI 136 TACGTG [CT]AC^GT[GA]
BsaBI 633 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 254 GAGACC GGTCTC(1/5)
BsmBI 171 CGTCTC CGTCTC(1/5)
BsmFI 235 GTCCC GGGAC(10/14)
BspLU11I 913 ACATGT A^CATGT
BsrFI 1162 GCCGGC [GA]^CCGG[CT]
BssSI 341 CTCGTG CACGAG(-5/-1)
BstF5I 972 GGATG GGATG(2/0)
BstYI 1014 GGATCC [GA]^GATC[CT]
Cfr10I 1162 GCCGGC [GA]^CCGG[CT]
DraI 1123 TTTAAA TTT^AAA
DraIII 1106 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 503 GAAGAG CTCTTC(1/4)
Eco47III 422 AGCGCT AGC^GCT
EcoT22I 882 ATGCAT ATGCA^T
FokI 972 GGATG GGATG(9/13)
KasI 243 GGCGCC G^GCGCC
Ksp632I 503 GAAGAG CTCTTC(1/4)
MaeII 137 ACGT A^CGT
MamI 633 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1014 GGATCC [GA]^GATC[CT]
MseI 1124 TTAA T^TAA
NaeI 1162 GCCGGC GCC^GGC
NarI 243 GGCGCC GG^CGCC
NcoI 892 CCATGG C^CATGG
NgoMIV 1162 GCCGGC G^CCGGC
NsiI 882 ATGCAT ATGCA^T
PpuMI 1188 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 1019 CAGCTG CAG^CTG
SfoI 243 GGCGCC GGC^GCC
SspI 657 AATATT AAT^ATT
Tru9I 1124 TTAA T^TAA
Tsp45I 1172 GTGAC ^GT[GC]AC
XhoII 1014 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 232 GGCGTC G[GA]^CG[CT]C
243 GGCGCC
AvaII 141 GGACC G^G[AT]CC
1189 GGACC
BalI 611 TGGCCA TGG^CCA
741 TGGCCA
BanI 243 GGCGCC G^G[CT][GA]CC
562 GGCACC
BsaHI 232 GGCGTC G[GA]^CG[CT]C
243 GGCGCC
BseRI 1001 CTCCTC GAGGAG(10/8)
418 GAGGAG
BsgI 351 CTGCAC GTGCAG(16/14)
806 GTGCAG
Bsp1407I 573 TGTACA T^GTACA
975 TGTACA
BsrDI 615 CATTGC GCAATG(2/0)
1151 CATTGC
BsrGI 573 TGTACA T^GTACA
975 TGTACA
BsrI 688 ACTGG ACTGG(1/-1)
538 CCAGT
BssHII 215 GCGCGC G^CGCGC
269 GCGCGC
DraII 85 GGGGCCC [GA]G^G[ACGT]CC[CT]
1188 AGGACCC
EcoO109I 85 GGGGCCC [GA]G^G[ACGT]CC[CT]
1188 AGGACCC
EcoRI 533 GAATTC G^AATTC
848 GAATTC
FauI 144 CCCGC CCCGC(4/6)
457 GCGGG
HaeII 243 GGCGCC [GA]GCGC^[CT]
422 AGCGCT
Hin1I 232 GGCGTC G[GA]^CG[CT]C
243 GGCGCC
MaeIII 541 GTAAC ^GT[ACGT]AC
1172 GTGAC
MboII 503 GAAGA GAAGA(8/7)
287 TCTTC
MluNI 611 TGGCCA TGG^CCA
741 TGGCCA
MscI 611 TGGCCA TGG^CCA
741 TGGCCA
MslI 624 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
627 CACCATGATG
MspA1I 588 CAGCGG C[AC]G^C[GT]G
1019 CAGCTG
NspI 570 GCATGT [GA]CATG^[CT]
913 ACATGT
PaeR7I 103 CTCGAG C^TCGAG
259 CTCGAG
PleI 1174 GACTC GAGTC(4/5)
1032 GAGTC
SmaI 82 CCCGGG CCC^GGG
89 CCCGGG
SmlI 103 CTCGAG C^T[CT][GA]AG
259 CTCGAG
SspBI 573 TGTACA T^GTACA
975 TGTACA
TfiI 997 GAATC G^A[AT]TC
1096 GAATC
TspRI 809 CAGTG CA[GC]TG[ACGT][ACGT]^
1108 CAGTG
XhoI 103 CTCGAG C^TCGAG
259 CTCGAG
XmaCI 82 CCCGGG C^CCGGG
89 CCCGGG
XmaI 82 CCCGGG C^CCGGG
89 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaI BanII BbvI
BcnI BfaI BglI BmyI BsaJI
BsiEI BsiYI BslI BsmAI BsoBI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EagI EclXI Eco52I EcoRII
EcoT14I Fnu4HI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MboI MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TseI
Tsp509I TthHB8I