(Circular) (Six-base) MAPSORT of: pFN21AB8710.seq Check: 2543 from: 1 to: 5905 pFN21AB8710 5905 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3715 278 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3248 1937 Size: 1311 4594 Acc65I G'GTAC_C Cuts at: 3226 3226 Size: 5905 AclI AA'CG_TT Cuts at: 1563 4032 4405 1563 Size: 2469 373 3063 Fragments arranged by size: 3063 2469 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3968 5352 5620 1632 Size: 2336 1384 268 1917 Fragments arranged by size: 2336 1917 1384 268 AfeI AGC'GCT Cuts at: 2429 2429 Size: 5905 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5708 AflIII A'CryG_T Cuts at: 1203 2918 4804 5781 1203 Size: 1715 1886 977 1327 Fragments arranged by size: 1886 1715 1327 977 AgeI A'CCGG_T Cuts at: 4736 4736 Size: 5905 AhdI GACnn_n'nnGTC Cuts at: 4634 4634 Size: 5905 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5905 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5905 AlwNI CAG_nnn'CTG Cuts at: 3648 5220 3648 Size: 1572 4333 ApaI G_GGCC'C Cuts at: 2095 2095 Size: 5905 ApaLI G'TGCA_C Cuts at: 3962 5118 3962 Size: 1156 4749 ApoI r'AATT_y Cuts at: 1493 2514 2538 2827 2853 3031 3214 3440 3592 Size: 1021 24 289 26 178 183 226 152 Cuts at: 3592 5701 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1021 289 226 183 178 152 26 24 AseI AT'TA_AT Cuts at: 160 1032 4459 160 Size: 872 3427 1606 Fragments arranged by size: 3427 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 AvaI C'yCGr_G Cuts at: 1870 1943 2087 2094 2108 2264 2300 3230 1870 Size: 73 144 7 14 156 36 930 4545 Fragments arranged by size: 4545 930 156 144 73 36 14 7 AvrII C'CTAG_G Cuts at: 2953 2953 Size: 5905 BamHI G'GATC_C Cuts at: 3019 3235 3602 3019 Size: 216 367 5322 Fragments arranged by size: 5322 367 216 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2248 2567 3226 4681 618 Size: 325 217 627 461 319 659 1455 1842 Fragments arranged by size: 1842 1455 659 627 461 325 319 217 BanII G_rGCy'C Cuts at: 1391 2041 2095 2107 2307 3224 1391 Size: 650 54 12 200 917 4072 Fragments arranged by size: 4072 917 650 200 54 12 BbeI G_GCGC'C Cuts at: 2252 2252 Size: 5905 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4934 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4096 4130 1903 Size: 34 2159 34 3678 Fragments arranged by size: 3678 2159 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3797 5007 3797 Size: 1210 4695 BclI T'GATC_A Cuts at: 1548 1548 Size: 5905 BfrBI ATG'CAT Cuts at: 2889 2889 Size: 5905 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2196 2235 2342 Size: 107 122 71 631 605 524 39 107 Cuts at: 2342 4516 136 Size: 2174 1525 Fragments arranged by size: 2174 1525 631 605 524 122 107 107 71 39 BglII A'GATC_T Cuts at: 5900 5900 Size: 5905 BlpI GC'TnA_GC Cuts at: 3322 3322 Size: 5905 Bme1580I G_kGCm'C Cuts at: 1790 2088 2095 2301 3966 5122 1790 Size: 298 7 206 1665 1156 2573 Fragments arranged by size: 2573 1665 1156 298 206 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5905 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3636 4594 442 Size: 416 53 83 399 24 2219 958 1753 Fragments arranged by size: 2219 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5905 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2134 3198 2134 Size: 1064 4841 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2982 4565 1239 Size: 1743 1583 2579 Fragments arranged by size: 2579 1743 1583 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4030 4895 5193 4030 Size: 865 298 4742 Fragments arranged by size: 4742 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2253 4568 882 Size: 617 754 2315 2219 Fragments arranged by size: 2315 2219 754 617 BsaAI yAC'GTr Cuts at: 493 1204 2143 3696 493 Size: 711 939 1553 2702 Fragments arranged by size: 2702 1553 939 711 BsaBI GATnn'nnATC Cuts at: 2642 5899 2642 Size: 3257 2648 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2238 2249 3712 4094 Size: 53 83 186 936 705 11 1463 382 Cuts at: 4094 4729 275 Size: 635 1451 Fragments arranged by size: 1463 1451 936 705 635 382 186 83 53 11 BsaWI w'CCGG_w Cuts at: 4338 4736 5010 5157 5568 4338 Size: 398 274 147 411 4675 Fragments arranged by size: 4675 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5680 Fragments arranged by size: 5680 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2438 2997 1186 Size: 1252 559 4094 Fragments arranged by size: 4094 1252 559 BseYI C'CCAG_C Cuts at: 3122 5108 5604 3122 Size: 1986 496 3423 Fragments arranged by size: 3423 1986 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2341 2832 1163 Size: 573 605 491 4236 Fragments arranged by size: 4236 605 573 491 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2124 2314 2406 3355 4116 5144 Size: 275 186 123 190 92 949 761 1028 Cuts at: 5144 1540 Size: 2301 Fragments arranged by size: 2301 1028 949 761 275 190 186 123 92 BsiHKAI G_wGCw'C Cuts at: 3966 4051 5122 3966 Size: 85 1071 4749 Fragments arranged by size: 4749 1071 85 BsmI GAATG_Cn' Cuts at: 3416 3509 3416 Size: 93 5812 BsmBI CGTCTCn'nnnn_ Cuts at: 2182 2182 Size: 5905 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2041 2088 2095 2107 2301 2307 3224 Size: 399 251 47 7 12 194 6 917 Cuts at: 3224 3966 4051 5122 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 917 742 399 251 194 85 47 12 7 6 BspEI T'CCGG_A Cuts at: 5568 5568 Size: 5905 BspHI T'CATG_A Cuts at: 3794 5524 3794 Size: 1730 4175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3260 844 Size: 2416 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3792 1394 Size: 429 1969 3507 Fragments arranged by size: 3507 1969 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2619 3155 4400 4574 65 Size: 1183 1371 536 1245 174 1396 Fragments arranged by size: 1396 1371 1245 1183 536 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3167 4549 4736 1700 Size: 110 1357 1382 187 2869 Fragments arranged by size: 2869 1382 1357 187 110 BsrGI T'GTAC_A Cuts at: 96 2578 2980 96 Size: 2482 402 3021 Fragments arranged by size: 3021 2482 402 BssHII G'CGCG_C Cuts at: 1925 2220 2274 1925 Size: 295 54 5556 Fragments arranged by size: 5556 295 54 BssSI C'ACGA_G Cuts at: 2346 3965 4977 2346 Size: 1619 1012 3274 Fragments arranged by size: 3274 1619 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5905 BstBI TT'CG_AA Cuts at: 4709 4709 Size: 5905 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5905 BstYI r'GATC_y Cuts at: 1972 3019 3235 3602 3987 4004 5456 5542 5554 Size: 1047 216 367 385 17 1452 86 12 Cuts at: 5554 5900 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1047 385 367 346 216 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2124 2243 2897 513 Size: 552 939 120 119 654 3521 Fragments arranged by size: 3521 939 654 552 120 119 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5166 Fragments arranged by size: 5166 683 56 BtsI GCAGTG_nn' Cuts at: 2820 3421 4215 4235 2820 Size: 601 794 20 4490 Fragments arranged by size: 4490 794 601 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5905 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5870 DraI TTT'AAA Cuts at: 3130 3209 3130 Size: 79 5826 DraIII CAC_nnn'GTG Cuts at: 3116 3116 Size: 5905 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4912 809 Size: 507 3596 1802 Fragments arranged by size: 3596 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2188 2311 Size: 54 1425 50 275 18 291 67 123 Cuts at: 2311 2403 2616 2746 3352 4241 5870 8 Size: 92 213 130 606 889 1629 43 Fragments arranged by size: 1629 1425 889 606 291 275 213 130 123 92 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2121 2311 2403 3352 1537 Size: 275 309 190 92 949 4090 Fragments arranged by size: 4090 949 309 275 190 92 EarI CTCTTCn'nnn_ Cuts at: 1353 2503 3835 1353 Size: 1150 1332 3423 Fragments arranged by size: 3423 1332 1150 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2271 3207 4492 4866 5012 387 Size: 1064 138 682 936 1285 374 146 1280 Fragments arranged by size: 1285 1280 1064 936 682 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2982 3968 4565 5352 5620 1239 Size: 393 1350 986 597 787 268 1524 Fragments arranged by size: 1524 1350 986 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2091 2091 Size: 5905 EcoRI G'AATT_C Cuts at: 2538 2853 3214 3592 2538 Size: 315 361 378 4851 Fragments arranged by size: 4851 378 361 315 FspI TGC'GCA Cuts at: 4411 4411 Size: 5905 HaeII r_GCGC'y Cuts at: 1678 2252 2431 5052 1678 Size: 574 179 2621 2531 Fragments arranged by size: 2621 2531 574 179 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2482 2514 2695 2727 2980 Size: 32 135 32 910 32 181 32 253 Cuts at: 2980 3012 4546 4578 4620 4652 1373 Size: 32 1534 32 42 32 2626 Fragments arranged by size: 2626 1534 910 253 181 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3249 3495 669 Size: 988 281 1311 246 3079 Fragments arranged by size: 3079 1311 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5905 HpaI GTT'AAC Cuts at: 3495 3495 Size: 5905 KasI G'GCGC_C Cuts at: 2248 2248 Size: 5905 KpnI G_GTAC'C Cuts at: 3230 3230 Size: 5905 MfeI C'AATT_G Cuts at: 3504 3504 Size: 5905 MluI A'CGCG_T Cuts at: 5781 5781 Size: 5905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5019 5203 1305 Size: 3714 184 2007 Fragments arranged by size: 3714 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2618 2748 10 Size: 54 1425 343 786 130 3167 Fragments arranged by size: 3167 1425 786 343 130 54 MslI CAynn'nnrTG Cuts at: 518 1166 2633 2636 3863 4222 4381 518 Size: 648 1467 3 1227 359 159 2042 Fragments arranged by size: 2042 1467 1227 648 359 159 3 MspA1I CmG'CkG Cuts at: 1606 2595 3026 3321 3998 5146 5391 1606 Size: 989 431 295 677 1148 245 2120 Fragments arranged by size: 2120 1148 989 677 431 295 245 NaeI GCC'GGC Cuts at: 3169 3169 Size: 5905 NarI GG'CG_CC Cuts at: 2249 2249 Size: 5905 NcoI C'CATG_G Cuts at: 513 1065 2004 2897 513 Size: 552 939 893 3521 Fragments arranged by size: 3521 939 893 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5905 NgoMIV G'CCGG_C Cuts at: 3167 3167 Size: 5905 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5905 NotI GC'GGCC_GC Cuts at: 3352 3352 Size: 5905 NsiI A_TGCA'T Cuts at: 2891 2891 Size: 5905 NspI r_CATG'y Cuts at: 1132 1229 2579 2922 3263 4808 1132 Size: 97 1350 343 341 1545 2229 Fragments arranged by size: 2229 1545 1350 343 341 97 PasI CC'CwG_GG Cuts at: 1348 2328 1348 Size: 980 4925 PciI A'CATG_T Cuts at: 2918 4804 2918 Size: 1886 4019 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 5003 PfoI T'CCnGG_A Cuts at: 3010 3010 Size: 5905 PmeI GTTT'AAAC Cuts at: 3209 3209 Size: 5905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3974 4006 5487 5519 3974 Size: 32 1481 32 4360 Fragments arranged by size: 4360 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5905 PsiI TTA'TAA Cuts at: 3475 3475 Size: 5905 PspOMI G'GGCC_C Cuts at: 2091 2091 Size: 5905 PspXI vC'TCGA_Gb Cuts at: 2264 2264 Size: 5905 PstI C_TGCA'G Cuts at: 830 3257 830 Size: 2427 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5905 PvuII CAG'CTG Cuts at: 3026 3026 Size: 5905 SalI G'TCGA_C Cuts at: 1936 3247 1936 Size: 1311 4594 SbfI CC_TGCA'GG Cuts at: 3257 3257 Size: 5905 ScaI AGT'ACT Cuts at: 4153 5865 4153 Size: 1712 4193 SfcI C'TryA_G Cuts at: 826 1045 3253 4388 5069 5260 826 Size: 219 2208 1135 681 191 1471 Fragments arranged by size: 2208 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2250 2250 Size: 5905 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5905 SmaI CCC'GGG Cuts at: 1872 2089 2096 3232 1872 Size: 217 7 1136 4545 Fragments arranged by size: 4545 1136 217 7 SmlI C'TyrA_G Cuts at: 820 1017 1943 2108 2264 4009 4910 5172 820 Size: 197 926 165 156 1745 901 262 1553 Fragments arranged by size: 1745 1553 926 901 262 197 165 156 SnaBI TAC'GTA Cuts at: 493 3696 493 Size: 3203 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5905 SphI G_CATG'C Cuts at: 1132 3263 1132 Size: 2131 3774 SspI AAT'ATT Cuts at: 5 52 2664 3829 5 Size: 47 2612 1165 2081 Fragments arranged by size: 2612 2081 1165 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5905 StyI C'CwwG_G Cuts at: 513 1065 2004 2211 2897 2953 3344 513 Size: 552 939 207 686 56 391 3074 Fragments arranged by size: 3074 939 686 552 391 207 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3932 3949 4102 4287 4732 325 Size: 1255 2352 17 153 185 445 1498 Fragments arranged by size: 2352 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2578 2980 4151 Size: 274 80 33 51 1443 601 402 1171 Cuts at: 4151 5863 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1171 601 402 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2463 2495 5865 5897 2463 Size: 32 3370 32 2471 Fragments arranged by size: 3370 2471 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5905 XbaI T'CTAG_A Cuts at: 3241 3241 Size: 5905 XhoI C'TCGA_G Cuts at: 1943 2108 2264 1943 Size: 165 156 5584 Fragments arranged by size: 5584 165 156 XmaI C'CCGG_G Cuts at: 1870 2087 2094 3230 1870 Size: 217 7 1136 4545 Fragments arranged by size: 4545 1136 217 7 XmnI GAAnn'nnTTC Cuts at: 4034 4034 Size: 5905 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3713 276 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI PacI PmlI PpuMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI