SPOCK1 ORF clone FHC02372

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC02372

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Clone info.

Species

human

Clone Name

pFN21AB7272 Sequence (with vector) Restriction Map

Product ID

FHC02372

Flexi Clone PID

FXC02372

ORF Sequence

DNA sequence (1326 bp)
Protein sequence (442 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463280]

Gene

KIBB7272

Symbol

SPOCK1

Alias : SPOCK, TESTICAN, TIC1

Description

sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1

Original Clone ID

bh00186m1

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1326 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 442 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4191.1 1.3e-164 99.3 SPOCK1 CCDS54817.1 2.9e-85 54.4 SPOCK3 CCDS34095.1 1.8e-83 53.7 SPOCK3 CCDS56344.1 5.2e-74 54.4 SPOCK3 CCDS7313.1 2.1e-67 47.3 SPOCK2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011497 142 183 PF07648 Protease inhibitor IPR019577 199 307 PF10591 SPARC/Testican IPR000716 316 379 PF00086 Thyroglobulin type-1 HMMSmart IPR002350 138 183 SM00280 Proteinase inhibitor I1 IPR000716 337 383 SM00211 Thyroglobulin type-1 ProfileScan NULL 133 184 PS51465 NULL IPR000716 313 379 PS51162 Thyroglobulin type-1
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MPAIAVLAAAAAAWCFLQVESRH 23 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_004589 439 602264 99.3 100.0 XP_011530320 436 607989 54.4 97.5 NP_058646 436 607989 54.4 97.5 XP_016863747 433 607989 53.7 97.5 NP_001035249 433 607989 53.7 97.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7272 Download

>KIBB7272  1326 bp
ATGCCGGCTATCGCGGTGTTGGCGGCGGCCGCCGCGGCGTGGTGCTTCCTCCAAGTCGAG
AGCCGGCACCTGGACGCGCTCGCCGGAGGCGCGGGCCCCAACCACGGCAATTTCCTAGAC
AATGACCAGTGGCTGAGCACCGTCTCCCAGTACGACCGGGACAAGTACTGGAACCGCTTT
CGAGACGAAGTTGAGGATGATTATTTCAGAAACTGGAATCCCAACAAGCCCTTTGACCAA
GCCCTGGACCCATCCAAGGACCCCTGCCTGAAGGTAAAATGCAGCCCTCACAAAGTGTGT
GTGACCCAGGACTACCAGACCGCCCTGTGTGTCAGCCGCAAGCACCTGCTCCCCAGGCAA
AAGAAGGGGAACGTGGCCCAGAAACACTGGGTTGGACCTTCGAATTTGGTCAAGTGCAAG
CCCTGTCCCGTGGCACAGTCAGCCATGGTCTGCGGCTCAGATGGCCACTCCTACACATCC
AAGTGCAAATTGGAGTTCCATGCTTGTTCTACTGGCAAAAGCCTCGCCACCCTCTGTGAT
GGGCCCTGTCCCTGTCTCCCAGAGCCTGAGCCACCAAAGCACAAGGCAGAAAGGAGTGCC
TGCACAGACAAGGAGTTGCGGAACCTTGCCTCCCGGCTGAAGGATTGGTTTGGAGCTCTC
CACGAGGATGCGAACAGAGTCATCAAGCCCACCAGCTCCAACACAGCCCAAGGCAGGTTT
GACACTAGCATCCTGCCCATCTGCAAGGACTCCCTGGGCTGGATGTTCAACAAGTTGGAC
ATGAACTATGACCTCCTGCTTGACCCTTCAGAGATCAATGCCATCTACCTGGATAAGTAC
GAGCCCTGTATCAAGCCTCTTTTCAACTCGTGTGACTCCTTCAAGGATGGCAAGCTTTCT
AACAATGAGTGGTGCTACTGCTTCCAGAAGCCTGGAGGTCTCCCTTGCCAGAATGAAATG
AACAGAATTCAGAAGCTGAGTAAGGGGAAAAGCCTGTTGGGGGCCTTCATACCTCGGTGT
AATGAGGAGGGCTATTACAAAGCCACACAGTGCCACGGCAGCACGGGGCAGTGCTGGTGT
GTGGACAAATATGGGAATGAGTTGGCTGGCTCCAGGAAACAGGGTGCTGTGAGCTGTGAA
GAGGAGCAGGAAACCTCAGGGGATTTTGGCAGTGGTGGGTCCGTGGTCCTGCTGGATGAC
CTAGAATATGAACGGGAGCTGGGACCAAAGGACAAAGAGGGGAAGCTGAGGGTGCACACC
CGAGCCGTGACAGAGGATGATGAGGATGAGGATGATGACAAAGAGGATGAGGTCGGGTAC
ATATGG

ORF Protein

Cloned ORF protein sequence for pFN21AB7272 Download

>KIBB7272  442 aa
MPAIAVLAAAAAAWCFLQVESRHLDALAGGAGPNHGNFLDNDQWLSTVSQYDRDKYWNRF
RDEVEDDYFRNWNPNKPFDQALDPSKDPCLKVKCSPHKVCVTQDYQTALCVSRKHLLPRQ
KKGNVAQKHWVGPSNLVKCKPCPVAQSAMVCGSDGHSYTSKCKLEFHACSTGKSLATLCD
GPCPCLPEPEPPKHKAERSACTDKELRNLASRLKDWFGALHEDANRVIKPTSSNTAQGRF
DTSILPICKDSLGWMFNKLDMNYDLLLDPSEINAIYLDKYEPCIKPLFNSCDSFKDGKLS
NNEWCYCFQKPGGLPCQNEMNRIQKLSKGKSLLGAFIPRCNEEGYYKATQCHGSTGQCWC
VDKYGNELAGSRKQGAVSCEEEQETSGDFGSGGSVVLLDDLEYERELGPKDKEGKLRVHT
RAVTEDDEDEDDDKEDEVGYIW

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7272 Download

>pFN21AB7272  6031 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCGGCTATCGCGGTGTTGGCGGCGGCCGCCGC
GGCGTGGTGCTTCCTCCAAGTCGAGAGCCGGCACCTGGACGCGCTCGCCGGAGGCGCGGG
CCCCAACCACGGCAATTTCCTAGACAATGACCAGTGGCTGAGCACCGTCTCCCAGTACGA
CCGGGACAAGTACTGGAACCGCTTTCGAGACGAAGTTGAGGATGATTATTTCAGAAACTG
GAATCCCAACAAGCCCTTTGACCAAGCCCTGGACCCATCCAAGGACCCCTGCCTGAAGGT
AAAATGCAGCCCTCACAAAGTGTGTGTGACCCAGGACTACCAGACCGCCCTGTGTGTCAG
CCGCAAGCACCTGCTCCCCAGGCAAAAGAAGGGGAACGTGGCCCAGAAACACTGGGTTGG
ACCTTCGAATTTGGTCAAGTGCAAGCCCTGTCCCGTGGCACAGTCAGCCATGGTCTGCGG
CTCAGATGGCCACTCCTACACATCCAAGTGCAAATTGGAGTTCCATGCTTGTTCTACTGG
CAAAAGCCTCGCCACCCTCTGTGATGGGCCCTGTCCCTGTCTCCCAGAGCCTGAGCCACC
AAAGCACAAGGCAGAAAGGAGTGCCTGCACAGACAAGGAGTTGCGGAACCTTGCCTCCCG
GCTGAAGGATTGGTTTGGAGCTCTCCACGAGGATGCGAACAGAGTCATCAAGCCCACCAG
CTCCAACACAGCCCAAGGCAGGTTTGACACTAGCATCCTGCCCATCTGCAAGGACTCCCT
GGGCTGGATGTTCAACAAGTTGGACATGAACTATGACCTCCTGCTTGACCCTTCAGAGAT
CAATGCCATCTACCTGGATAAGTACGAGCCCTGTATCAAGCCTCTTTTCAACTCGTGTGA
CTCCTTCAAGGATGGCAAGCTTTCTAACAATGAGTGGTGCTACTGCTTCCAGAAGCCTGG
AGGTCTCCCTTGCCAGAATGAAATGAACAGAATTCAGAAGCTGAGTAAGGGGAAAAGCCT
GTTGGGGGCCTTCATACCTCGGTGTAATGAGGAGGGCTATTACAAAGCCACACAGTGCCA
CGGCAGCACGGGGCAGTGCTGGTGTGTGGACAAATATGGGAATGAGTTGGCTGGCTCCAG
GAAACAGGGTGCTGTGAGCTGTGAAGAGGAGCAGGAAACCTCAGGGGATTTTGGCAGTGG
TGGGTCCGTGGTCCTGCTGGATGACCTAGAATATGAACGGGAGCTGGGACCAAAGGACAA
AGAGGGGAAGCTGAGGGTGCACACCCGAGCCGTGACAGAGGATGATGAGGATGAGGATGA
TGACAAAGAGGATGAGGTCGGGTACATATGGGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT