(Circular) (Six-base) MAPSORT of: pFN21AB7272.seq Check: 3323 from: 1 to: 6031 pFN21AB7272 6031 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2358 2358 Size: 6031 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3841 278 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3374 1937 Size: 1437 4594 Acc65I G'GTAC_C Cuts at: 3352 3352 Size: 6031 AclI AA'CG_TT Cuts at: 1563 4158 4531 1563 Size: 2595 373 3063 Fragments arranged by size: 3063 2595 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2294 2663 2796 4094 5478 5746 1632 Size: 662 369 133 1298 1384 268 1917 Fragments arranged by size: 1917 1384 1298 662 369 268 133 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5834 AflIII A'CryG_T Cuts at: 1203 4930 5907 1203 Size: 3727 977 1327 Fragments arranged by size: 3727 1327 977 AgeI A'CCGG_T Cuts at: 4862 4862 Size: 6031 AhdI GACnn_n'nnGTC Cuts at: 4760 4760 Size: 6031 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6031 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2366 1184 Size: 1182 4849 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2618 2650 2618 Size: 32 5999 AlwNI CAG_nnn'CTG Cuts at: 2137 2217 2570 2980 3774 5346 2137 Size: 80 353 410 794 1572 2822 Fragments arranged by size: 2822 1572 794 410 353 80 ApaI G_GGCC'C Cuts at: 2102 2550 2102 Size: 448 5583 ApaLI G'TGCA_C Cuts at: 3257 4088 5244 3257 Size: 831 1156 4044 Fragments arranged by size: 4044 1156 831 ApoI r'AATT_y Cuts at: 1493 2407 2970 3340 3566 3718 5827 1493 Size: 914 563 370 226 152 2109 1697 Fragments arranged by size: 2109 1697 914 563 370 226 152 AseI AT'TA_AT Cuts at: 160 1032 4585 160 Size: 872 3553 1606 Fragments arranged by size: 3553 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 AvaI C'yCGr_G Cuts at: 1870 1943 3264 3356 1870 Size: 73 1321 92 4545 Fragments arranged by size: 4545 1321 92 73 BamHI G'GATC_C Cuts at: 3361 3728 3361 Size: 367 5664 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2070 3352 4807 618 Size: 325 217 627 283 1282 1455 1842 Fragments arranged by size: 1842 1455 1282 627 325 283 217 BanII G_rGCy'C Cuts at: 1391 2102 2550 2662 2850 3350 1391 Size: 711 448 112 188 500 4072 Fragments arranged by size: 4072 711 500 448 188 112 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5060 BbvCI CC'TCA_GC Cuts at: 3251 3251 Size: 6031 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2509 2543 4222 4256 1903 Size: 34 50 34 488 34 1679 34 3678 Fragments arranged by size: 3678 1679 488 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3923 5133 3923 Size: 1210 4821 BclI T'GATC_A Cuts at: 1548 1548 Size: 6031 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2039 4642 Size: 107 122 71 631 605 337 30 2603 Cuts at: 4642 136 Size: 1525 Fragments arranged by size: 2603 1525 631 605 337 122 107 71 30 BglII A'GATC_T Cuts at: 6026 6026 Size: 6031 BlpI GC'TnA_GC Cuts at: 2138 3448 2138 Size: 1310 4721 Bme1580I G_kGCm'C Cuts at: 1790 2102 2550 3261 4092 5248 1790 Size: 312 448 711 831 1156 2573 Fragments arranged by size: 2573 1156 831 711 448 312 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6031 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2146 2401 3762 Size: 416 53 83 399 24 729 255 1361 Cuts at: 3762 4720 442 Size: 958 1753 Fragments arranged by size: 1753 1361 958 729 416 399 255 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6031 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2958 3100 4691 1239 Size: 1719 142 1591 2579 Fragments arranged by size: 2579 1719 1591 142 Bpu10I CC'TnA_GC Cuts at: 2571 3251 2571 Size: 680 5351 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4156 5021 5319 4156 Size: 865 298 4868 Fragments arranged by size: 4868 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2948 4694 882 Size: 617 1449 1746 2219 Fragments arranged by size: 2219 1746 1449 617 BsaAI yAC'GTr Cuts at: 493 1204 3822 493 Size: 711 2618 2702 Fragments arranged by size: 2702 2618 711 BsaBI GATnn'nnATC Cuts at: 6025 6025 Size: 6031 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3838 4220 4855 275 Size: 53 83 186 936 2305 382 635 1451 Fragments arranged by size: 2305 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4464 4862 5136 5283 5694 4464 Size: 398 274 147 411 4801 Fragments arranged by size: 4801 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2618 2648 1178 Size: 30 165 30 1215 30 4561 Fragments arranged by size: 4561 1215 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3044 3161 1186 Size: 1858 117 4056 Fragments arranged by size: 4056 1858 117 BseYI C'CCAG_C Cuts at: 3223 5234 5730 3223 Size: 2011 496 3524 Fragments arranged by size: 3524 2011 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2590 1163 Size: 573 854 4604 Fragments arranged by size: 4604 854 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 2161 3481 4242 5270 1540 Size: 275 186 33 127 1320 761 1028 2301 Fragments arranged by size: 2301 1320 1028 761 275 186 127 33 BsiHKAI G_wGCw'C Cuts at: 2144 2662 3261 4092 4177 5248 2144 Size: 518 599 831 85 1071 2927 Fragments arranged by size: 2927 1071 831 599 518 85 BsmI GAATG_Cn' Cuts at: 3542 3635 3542 Size: 93 5938 BsmBI CGTCTCn'nnnn_ Cuts at: 2152 2181 2152 Size: 29 6002 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2102 2144 2550 2662 2850 3261 3350 Size: 399 312 42 406 112 188 411 89 Cuts at: 3350 4092 4177 5248 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 411 406 399 312 188 112 89 85 42 BspEI T'CCGG_A Cuts at: 5694 5694 Size: 6031 BspHI T'CATG_A Cuts at: 3920 5650 3920 Size: 1730 4301 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2358 2709 3386 844 Size: 1514 351 677 3489 Fragments arranged by size: 3489 1514 677 351 BsrBI CCG'CTC Cuts at: 1394 1823 3918 1394 Size: 429 2095 3507 Fragments arranged by size: 3507 2095 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4526 4700 65 Size: 1183 3278 174 1396 Fragments arranged by size: 3278 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 2067 4675 4862 1700 Size: 110 198 59 2608 187 2869 Fragments arranged by size: 2869 2608 198 187 110 59 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6031 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6031 BssSI C'ACGA_G Cuts at: 2666 2872 4091 5103 2666 Size: 206 1219 1012 3594 Fragments arranged by size: 3594 1219 1012 206 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6031 BstBI TT'CG_AA Cuts at: 2405 4835 2405 Size: 2430 3601 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6031 BstXI CCAn_nnnn'nTGG Cuts at: 2833 2936 2833 Size: 103 5928 BstYI r'GATC_y Cuts at: 1972 3361 3728 4113 4130 5582 5668 5680 6026 Size: 1389 367 385 17 1452 86 12 346 Cuts at: 6026 1972 Size: 1977 Fragments arranged by size: 1977 1452 1389 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3160 3160 Size: 6031 BtgI C'CryG_G Cuts at: 513 1065 2037 2107 2433 2448 3058 3186 513 Size: 552 972 70 326 15 610 128 3358 Fragments arranged by size: 3358 972 610 552 326 128 70 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5292 Fragments arranged by size: 5292 683 56 BtsI GCAGTG_nn' Cuts at: 3080 3181 3547 4341 4361 3080 Size: 101 366 794 20 4750 Fragments arranged by size: 4750 794 366 101 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6031 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5996 DraI TTT'AAA Cuts at: 3335 3335 Size: 6031 DraIII CAC_nnn'GTG Cuts at: 2299 3054 2299 Size: 755 5276 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5038 809 Size: 507 3722 1802 Fragments arranged by size: 3722 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2467 3478 Size: 54 1425 50 275 18 201 436 1011 Cuts at: 3478 4367 5996 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1011 889 436 275 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2031 3478 1537 Size: 275 219 1447 4090 Fragments arranged by size: 4090 1447 275 219 EarI CTCTTCn'nnn_ Cuts at: 1353 3138 3961 1353 Size: 1785 823 3423 Fragments arranged by size: 3423 1785 823 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4618 4992 5138 387 Size: 1064 138 3029 374 146 1280 Fragments arranged by size: 3029 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2660 2660 Size: 6031 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 2663 2796 2958 3100 4094 4691 Size: 393 662 369 133 162 142 994 597 Cuts at: 4691 5478 5746 1239 Size: 787 268 1524 Fragments arranged by size: 1524 994 787 662 597 393 369 268 162 142 133 EcoO109I rG'GnC_Cy Cuts at: 2099 2263 2547 3006 2099 Size: 164 284 459 5124 Fragments arranged by size: 5124 459 284 164 EcoRI G'AATT_C Cuts at: 2970 3340 3718 2970 Size: 370 378 5283 Fragments arranged by size: 5283 378 370 FspI TGC'GCA Cuts at: 4537 4537 Size: 6031 HaeII r_GCGC'y Cuts at: 1678 5178 1678 Size: 3500 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2456 2488 4672 4704 4746 Size: 32 135 32 884 32 2184 32 42 Cuts at: 4746 4778 1373 Size: 32 2626 Fragments arranged by size: 2626 2184 884 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3375 3621 669 Size: 988 281 1437 246 3079 Fragments arranged by size: 3079 1437 988 281 246 HindIII A'AGCT_T Cuts at: 748 2897 748 Size: 2149 3882 HpaI GTT'AAC Cuts at: 3621 3621 Size: 6031 KpnI G_GTAC'C Cuts at: 3356 3356 Size: 6031 MfeI C'AATT_G Cuts at: 3630 3630 Size: 6031 MluI A'CGCG_T Cuts at: 5907 5907 Size: 6031 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2377 2727 2760 5145 5329 1305 Size: 1072 350 33 2385 184 2007 Fragments arranged by size: 2385 2007 1072 350 184 33 MscI TGG'CCA Cuts at: 10 64 1489 1832 2469 10 Size: 54 1425 343 637 3572 Fragments arranged by size: 3572 1425 637 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2485 2670 3989 4348 4507 518 Size: 648 1319 185 1319 359 159 2042 Fragments arranged by size: 2042 1319 1319 648 359 185 159 MspA1I CmG'CkG Cuts at: 1606 2039 3447 4124 5272 5517 1606 Size: 433 1408 677 1148 245 2120 Fragments arranged by size: 2120 1408 1148 677 433 245 NaeI GCC'GGC Cuts at: 2010 2069 2010 Size: 59 5972 NcoI C'CATG_G Cuts at: 513 1065 2448 513 Size: 552 1383 4096 Fragments arranged by size: 4096 1383 552 NdeI CA'TA_TG Cuts at: 387 3326 387 Size: 2939 3092 NgoMIV G'CCGG_C Cuts at: 2008 2067 2008 Size: 59 5972 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6031 NotI GC'GGCC_GC Cuts at: 2031 3478 2031 Size: 1447 4584 NspI r_CATG'y Cuts at: 1132 1229 3389 4934 1132 Size: 97 2160 1545 2229 Fragments arranged by size: 2229 2160 1545 97 PasI CC'CwG_GG Cuts at: 1348 2758 1348 Size: 1410 4621 PciI A'CATG_T Cuts at: 4930 4930 Size: 6031 PflMI CCAn_nnn'nTGG Cuts at: 1108 2248 1108 Size: 1140 4891 PfoI T'CCnGG_A Cuts at: 3116 3116 Size: 6031 PmeI GTTT'AAAC Cuts at: 3335 3335 Size: 6031 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2618 2650 4100 4132 5613 5645 2618 Size: 32 1450 32 1481 32 3004 Fragments arranged by size: 3004 1481 1450 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2263 2263 Size: 6031 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6031 PsiI TTA'TAA Cuts at: 3601 3601 Size: 6031 PspOMI G'GGCC_C Cuts at: 2098 2546 2098 Size: 448 5583 PstI C_TGCA'G Cuts at: 830 3383 830 Size: 2553 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6031 SacI G_AGCT'C Cuts at: 2662 2662 Size: 6031 SacII CC_GC'GG Cuts at: 2040 2040 Size: 6031 SalI G'TCGA_C Cuts at: 1936 3373 1936 Size: 1437 4594 SbfI CC_TGCA'GG Cuts at: 3383 3383 Size: 6031 ScaI AGT'ACT Cuts at: 2171 4279 5991 2171 Size: 2108 1712 2211 Fragments arranged by size: 2211 2108 1712 SfcI C'TryA_G Cuts at: 826 1045 3379 4514 5195 5386 826 Size: 219 2334 1135 681 191 1471 Fragments arranged by size: 2334 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6031 SmaI CCC'GGG Cuts at: 1872 3358 1872 Size: 1486 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4135 5036 5298 820 Size: 197 926 2192 901 262 1553 Fragments arranged by size: 2192 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3822 493 Size: 3329 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6031 SphI G_CATG'C Cuts at: 1132 3389 1132 Size: 2257 3774 SspI AAT'ATT Cuts at: 5 52 3955 5 Size: 47 3903 2081 Fragments arranged by size: 3903 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6031 StyI C'CwwG_G Cuts at: 513 1065 2259 2448 2713 3470 513 Size: 552 1194 189 265 757 3074 Fragments arranged by size: 3074 1194 757 552 265 189 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4058 4075 4228 4413 4858 325 Size: 1255 2478 17 153 185 445 1498 Fragments arranged by size: 2478 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2169 4277 5989 Size: 274 80 33 51 1443 192 2108 1712 Cuts at: 5989 96 Size: 138 Fragments arranged by size: 2108 1712 1443 274 192 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3112 3144 5991 6023 3112 Size: 32 2847 32 3120 Fragments arranged by size: 3120 2847 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6031 XbaI T'CTAG_A Cuts at: 3367 3367 Size: 6031 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2491 2491 Size: 6031 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6031 XmaI C'CCGG_G Cuts at: 1870 3356 1870 Size: 1486 4545 XmnI GAAnn'nnTTC Cuts at: 4160 4160 Size: 6031 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3839 276 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI