Restriction map for
pFN21AB7272
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AlwI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbsI
BciVI BclI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII Bst1107I BstAPI BstEII
BstPI BstYI BstZ17I ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII Hin1I HincII HindII
HpaI HphI KasI KpnI MamI
MfeI MflI MluI MroI MseI
MunI NarI NheI NruI NsiI
NspI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SalI SapI SbfI SexAI
SfcI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Alw44I 1252 GTGCAC G^TGCAC
AocI 1154 CCTCAGG CC^T[ACGT]AGG
ApaLI 1252 GTGCAC G^TGCAC
AvaI 1259 CCCGAG C^[CT]CG[GA]G
BalI 462 TGGCCA TGG^CCA
BanI 65 GGCACC G^G[CT][GA]CC
BbvCI 1245 GCTGAGG CCTCAGC(-5/-2)
BcgI 515 GCAAAAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 28 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 132 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 132 GCTGAGC GC^T[ACGT]AGC
BsaI 937 GGTCTC GGTCTC(1/5)
BsgI 600 CTGCAC GTGCAG(16/14)
BsoBI 1259 CCCGAG C^[CT]CG[GA]G
BstBI 399 TTCGAA TT^CGAA
Bsu36I 1154 CCTCAGG CC^T[ACGT]AGG
CelII 132 GCTGAGC GC^T[ACGT]AGC
DpnII 813 GATC ^GATC
EagI 26 CGGCCG C^GGCCG
EarI 1138 GAAGAG CTCTTC(1/4)
EclXI 26 CGGCCG C^GGCCG
Eco52I 26 CGGCCG C^GGCCG
Eco81I 1154 CCTCAGG CC^T[ACGT]AGG
EcoRI 965 GAATTC G^AATTC
FauI 91 GCGGG CCCGC(4/6)
HgaI 73 GACGC GACGC(5/10)
HindIII 892 AAGCTT A^AGCTT
Ksp632I 1138 GAAGAG CTCTTC(1/4)
KspI 32 CCGCGG CCGC^GG
MaeII 371 ACGT A^CGT
MboI 813 GATC ^GATC
MboII 1138 GAAGA GAAGA(8/7)
MluNI 462 TGGCCA TGG^CCA
MscI 462 TGGCCA TGG^CCA
MspA1I 32 CCGCGG C[AC]G^C[GT]G
NcoI 443 CCATGG C^CATGG
NdeI 1320 CATATG CA^TATG
NdeII 813 GATC ^GATC
NotI 25 GCGGCCGC GC^GGCCGC
NspV 399 TTCGAA TT^CGAA
PflMI 237 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 257 AGGACCC [GA]G^G[AT]CC[CT]
SacI 653 GAGCTC GAGCT^C
SacII 32 CCGCGG CCGC^GG
Sau3AI 813 GATC ^GATC
ScaI 164 AGTACT AGT^ACT
SfuI 399 TTCGAA TT^CGAA
TfiI 216 GAATC G^A[AT]TC
Van91I 237 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 479 CCAAGTGCAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 402 GAATTT [GA]^AATT[CT]
965 GAATTC
ApaI 93 GGGCCC GGGCC^C
541 GGGCCC
ApoI 402 GAATTT [GA]^AATT[CT]
965 GAATTC
BbvI 281 GCAGC GCAGC(8/12)
1058 GCAGC
BcnI 156 CCGGG CC^[GC]GG
632 CCCGG
BpmI 1110 CTCCAG CTGGAG(16/14)
932 CTGGAG
BseRI 1024 GAGGAG GAGGAG(10/8)
1141 GAGGAG
BsiEI 26 CGGCCG CG[GA][CT]^CG
153 CGACCG
BsmBI 141 CGTCTC CGTCTC(1/5)
182 GAGACG
BspMI 344 ACCTGC ACCTGC(4/8)
713 GCAGGT
BsrFI 3 GCCGGC [GA]^CCGG[CT]
62 GCCGGC
BssSI 661 CACGAG CACGAG(-5/-1)
867 CTCGTG
BstXI 821 CCATCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
924 CCAGAAGCCTGG
CfoI 76 GCGC GCG^C
89 GCGC
Cfr10I 3 GCCGGC [GA]^CCGG[CT]
62 GCCGGC
DraIII 289 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1044 CACACAGTG
EaeI 26 CGGCCG [CT]^GGCC[GA]
462 TGGCCA
HhaI 76 GCGC GCG^C
89 GCGC
HinP1I 76 GCGC G^CGC
89 GCGC
MslI 476 CATCCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
661 CACGAGGATG
NaeI 3 GCCGGC GCC^GGC
62 GCCGGC
NciI 156 CCGGG CC^[GC]GG
632 CCCGG
NgoMIV 3 GCCGGC G^CCGGC
62 GCCGGC
SfaNI 667 GATGC GCATC(5/9)
728 GCATC
TseI 281 GCAGC G^C[AT]GC
1058 GCAGC
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BanII BfaI BmyI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I Cfr13I DdeI
DraII DsaI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MaeI MaeIII MnlI
MspI MvaI MvnI MwoI NlaIII
NlaIV PleI RsaI Sau96I ScrFI
StyI TaqI Tsp45I Tsp509I TspRI
TthHB8I