Restriction map for
pFN21AB7272
(Linear) (Six-base) MAPSORT of: KIBB7272.seq Check: 3933 from: 1 to: 1326
KIBB7272.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 353 1326
Size: 353 973
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 658 791 1326
Size: 289 369 133 535
Fragments arranged by size:
535 369 289 133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 361 1326
Size: 361 965
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 613 645 1326
Size: 613 32 681
Fragments arranged by size:
681 613 32
AlwNI CAG_nnn'CTG
Cuts at: 0 132 212 565 975 1326
Size: 132 80 353 410 351
Fragments arranged by size:
410 353 351 132 80
ApaI G_GGCC'C
Cuts at: 0 97 545 1326
Size: 97 448 781
Fragments arranged by size:
781 448 97
ApaLI G'TGCA_C
Cuts at: 0 1252 1326
Size: 1252 74
ApoI r'AATT_y
Cuts at: 0 402 965 1326
Size: 402 563 361
Fragments arranged by size:
563 402 361
AvaI C'yCGr_G
Cuts at: 0 1259 1326
Size: 1259 67
BanI G'GyrC_C
Cuts at: 0 65 1326
Size: 65 1261
BanII G_rGCy'C
Cuts at: 0 97 545 657 845 1326
Size: 97 448 112 188 481
Fragments arranged by size:
481 448 188 112 97
BbvCI CC'TCA_GC
Cuts at: 0 1246 1326
Size: 1246 80
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 504 538 1326
Size: 504 34 788
Fragments arranged by size:
788 504 34
BglI GCCn_nnn'nGGC
Cuts at: 0 34 1326
Size: 34 1292
BlpI GC'TnA_GC
Cuts at: 0 133 1326
Size: 133 1193
Bme1580I G_kGCm'C
Cuts at: 0 97 545 1256 1326
Size: 97 448 711 70
Fragments arranged by size:
711 448 97 70
BmrI ACTGGGnnnn_n'
Cuts at: 0 141 396 1326
Size: 141 255 930
Fragments arranged by size:
930 255 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 953 1095 1326
Size: 953 142 231
Fragments arranged by size:
953 231 142
Bpu10I CC'TnA_GC
Cuts at: 0 566 1246 1326
Size: 566 680 80
Fragments arranged by size:
680 566 80
BsaI GGTCTCn'nnnn_
Cuts at: 0 943 1326
Size: 943 383
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 613 643 1326
Size: 613 30 683
Fragments arranged by size:
683 613 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1039 1156 1326
Size: 1039 117 170
Fragments arranged by size:
1039 170 117
BseYI C'CCAG_C
Cuts at: 0 1218 1326
Size: 1218 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 585 1326
Size: 585 741
BsiEI CG_ry'CG
Cuts at: 0 29 156 1326
Size: 29 127 1170
Fragments arranged by size:
1170 127 29
BsiHKAI G_wGCw'C
Cuts at: 0 139 657 1256 1326
Size: 139 518 599 70
Fragments arranged by size:
599 518 139 70
BsmBI CGTCTCn'nnnn_
Cuts at: 0 147 176 1326
Size: 147 29 1150
Fragments arranged by size:
1150 147 29
Bsp1286I G_dGCh'C
Cuts at: 0 97 139 545 657 845 1256 1326
Size: 97 42 406 112 188 411 70
Fragments arranged by size:
411 406 188 112 97 70 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 353 704 1326
Size: 353 351 622
Fragments arranged by size:
622 353 351
BsrFI r'CCGG_y
Cuts at: 0 3 62 1326
Size: 3 59 1264
Fragments arranged by size:
1264 59 3
BssSI C'ACGA_G
Cuts at: 0 661 867 1326
Size: 661 206 459
Fragments arranged by size:
661 459 206
BstBI TT'CG_AA
Cuts at: 0 400 1326
Size: 400 926
BstXI CCAn_nnnn'nTGG
Cuts at: 0 828 931 1326
Size: 828 103 395
Fragments arranged by size:
828 395 103
Bsu36I CC'TnA_GG
Cuts at: 0 1155 1326
Size: 1155 171
BtgI C'CryG_G
Cuts at: 0 32 102 428 443 1053 1181 1326
Size: 32 70 326 15 610 128 145
Fragments arranged by size:
610 326 145 128 70 32 15
BtsI GCAGTG_nn'
Cuts at: 0 1075 1176 1326
Size: 1075 101 150
Fragments arranged by size:
1075 150 101
DraIII CAC_nnn'GTG
Cuts at: 0 294 1049 1326
Size: 294 755 277
Fragments arranged by size:
755 294 277
EaeI y'GGCC_r
Cuts at: 0 26 462 1326
Size: 26 436 864
Fragments arranged by size:
864 436 26
EagI C'GGCC_G
Cuts at: 0 26 1326
Size: 26 1300
EarI CTCTTCn'nnn_
Cuts at: 0 1133 1326
Size: 1133 193
EcoICRI GAG'CTC
Cuts at: 0 655 1326
Size: 655 671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 658 791 953 1095 1326
Size: 289 369 133 162 142 231
Fragments arranged by size:
369 289 231 162 142 133
EcoO109I rG'GnC_Cy
Cuts at: 0 94 258 542 1001 1326
Size: 94 164 284 459 325
Fragments arranged by size:
459 325 284 164 94
EcoRI G'AATT_C
Cuts at: 0 965 1326
Size: 965 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 451 483 1326
Size: 451 32 843
Fragments arranged by size:
843 451 32
HindIII A'AGCT_T
Cuts at: 0 892 1326
Size: 892 434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 722 755 1326
Size: 372 350 33 571
Fragments arranged by size:
571 372 350 33
MscI TGG'CCA
Cuts at: 0 464 1326
Size: 464 862
MslI CAynn'nnrTG
Cuts at: 0 480 665 1326
Size: 480 185 661
Fragments arranged by size:
661 480 185
MspA1I CmG'CkG
Cuts at: 0 34 1326
Size: 34 1292
NaeI GCC'GGC
Cuts at: 0 5 64 1326
Size: 5 59 1262
Fragments arranged by size:
1262 59 5
NcoI C'CATG_G
Cuts at: 0 443 1326
Size: 443 883
NdeI CA'TA_TG
Cuts at: 0 1321 1326
Size: 1321 5
NgoMIV G'CCGG_C
Cuts at: 0 3 62 1326
Size: 3 59 1264
Fragments arranged by size:
1264 59 3
NotI GC'GGCC_GC
Cuts at: 0 26 1326
Size: 26 1300
PasI CC'CwG_GG
Cuts at: 0 753 1326
Size: 753 573
PflMI CCAn_nnn'nTGG
Cuts at: 0 243 1326
Size: 243 1083
PfoI T'CCnGG_A
Cuts at: 0 1111 1326
Size: 1111 215
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 613 645 1326
Size: 613 32 681
Fragments arranged by size:
681 613 32
PpuMI rG'GwC_Cy
Cuts at: 0 258 1326
Size: 258 1068
PspOMI G'GGCC_C
Cuts at: 0 93 541 1326
Size: 93 448 785
Fragments arranged by size:
785 448 93
SacI G_AGCT'C
Cuts at: 0 657 1326
Size: 657 669
SacII CC_GC'GG
Cuts at: 0 35 1326
Size: 35 1291
ScaI AGT'ACT
Cuts at: 0 166 1326
Size: 166 1160
StyI C'CwwG_G
Cuts at: 0 254 443 708 1326
Size: 254 189 265 618
Fragments arranged by size:
618 265 254 189
TatI w'GTAC_w
Cuts at: 0 164 1326
Size: 164 1162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1107 1139 1326
Size: 1107 32 187
Fragments arranged by size:
1107 187 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 486 1326
Size: 486 840
Enzymes that do cut:
AarI AcuI AlfI AloI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbvCI BcgI BglI BlpI Bme1580I
BmrI BpmI Bpu10I BsaI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssSI BstBI
BstXI Bsu36I BtgI BtsI DraIII EaeI EagI EarI
EcoICRI Eco57MI EcoO109I EcoRI Hin4I HindIII MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NotI PasI
PflMI PfoI PpiI PpuMI PspOMI SacI SacII ScaI
StyI TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BciVI BclI BfrBI BglII BmgBI BmtI
BplI BpuEI BsaAI BsaBI BsaHI BsaWI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstEII
BstYI BstZ17I BtgZI ClaI CspCI DraI DrdI EciI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI NarI NheI NruI
NsiI NspI PacI PciI PmeI PmlI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI
SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XhoI XmaI XmnI ZraI