EDEM1 ORF clone FXC00461

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Flexi ORF Clone FXC00461

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Clone info.

Species

human

Clone Name

pF1KSDA0212 Sequence (with vector) Restriction Map

Product ID

FXC00461

HaloTag Clone PID

FHC00461

ORF Sequence

DNA sequence (1971 bp)
Protein sequence (657 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383802]

Gene

KIAA0212

Symbol

EDEM1

Alias : EDEM

Description

ER degradation enhancer, mannosidase alpha-like 1

Original Clone ID

ha02602

Features of the cloned ORF sequence

Description

Length: 1971 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 657 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33686.1 9e-180 100.0 EDEM1 CCDS1363.2 1.1e-28 44.8 EDEM3 CCDS46592.1 1.5e-14 41.2 EDEM2 CCDS13247.1 1.5e-14 40.8 EDEM2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001382 137 157 PR00747 Glycoside hydrolase IPR001382 183 197 PR00747 Glycoside hydrolase IPR001382 220 238 PR00747 Glycoside hydrolase IPR001382 274 293 PR00747 Glycoside hydrolase IPR001382 366 383 PR00747 Glycoside hydrolase IPR001382 434 450 PR00747 Glycoside hydrolase IPR001382 489 513 PR00747 Glycoside hydrolase IPR001382 548 568 PR00747 Glycoside hydrolase HMMPfam IPR001382 137 584 PF01532 Glycoside hydrolase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 7 VLGLVLLRLGLHGVLWLVFGLGP 29 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055489 657 607673 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KSDA0212 Download

>KIAA0212  1971 bp
ATGCAATGGCGAGCGCTCGTCCTGGGGCTGGTGCTCCTCCGGCTTGGCCTCCATGGAGTA
TTGTGGCTCGTCTTCGGGCTGGGGCCCAGCATGGGCTTCTACCAGCGCTTTCCGCTCAGC
TTCGGCTTCCAGCGTCTGAGGAGCCCCGACGGCCCCGCGTCGCCCACCTCGGGGCCCGTG
GGCCGGCCTGGGGGGGTATCCGGGCCGTCGTGGCTGCAGCCGCCGGGGACCGGGGCAGCG
CAGAGCCCGCGCAAGGCTCCGCGGCGTCCTGGGCCGGGGATGTGCGGCCCAGCCAACTGG
GGCTACGTGCTGGGCGGCCGGGGCCGCGGCCCGGACGAGTACGAGAAGCGCTACAGCGGC
GCCTTCCCTCCGCAGCTGCGTGCCCAGATGCGCGACCTGGCACGGGGCATGTTCGTCTTT
GGCTACGACAACTACATGGCTCACGCCTTCCCCCAGGACGAGCTCAACCCCATCCACTGC
CGCGGCCGTGGGCCCGACCGCGGGGACCCTTCAAATCTGAACATCAATGATGTACTAGGG
AACTACTCATTGACTCTTGTTGATGCATTGGATACACTTGCAATAATGGGAAATTCATCC
GAGTTCCAGAAAGCAGTCAAGTTAGTGATCAACACAGTTTCATTTGACAAAGATTCCACC
GTCCAAGTCTTTGAGGCCACGATAAGGGTCCTGGGAAGCCTCCTTTCTGCTCACAGAATA
ATAACTGACTCCAAGCAGCCCTTTGGTGACATGACAATTAAGGACTATGATAATGAGTTG
TTATACATGGCCCATGACCTGGCGGTGCGGCTCCTCCCTGCTTTTGAAAACACCAAGACA
GGGATTCCATATCCTCGGGTGAATCTAAAGACAGGAGTTCCTCCTGACACCAATAATGAG
ACATGCACAGCGGGAGCCGGTTCCCTCCTGGTGGAATTTGGGATTCTGAGTCGACTCCTG
GGGGACTCCACATTTGAGTGGGTGGCCAGACGAGCAGTGAAAGCCCTTTGGAACCTCCGG
AGCAATGATACAGGATTACTAGGCAATGTCGTGAACATTCAGACGGGCCACTGGGTTGGA
AAGCAGAGTGGCCTGGGTGCCGGGCTGGACTCCTTCTATGAATACCTCTTGAAATCTTAC
ATTCTCTTTGGAGAAAAAGAAGACCTAGAAATGTTTAATGCTGCATATCAGAGTATTCAG
AACTACTTAAGAAGAGGGCGGGAAGCCTGCAATGAAGGAGAAGGAGACCCTCCACTCTAT
GTCAACGTGAACATGTTCAGTGGGCAGCTGATGAACACCTGGATTGACTCTCTGCAGGCC
TTTTTCCCTGGACTGCAGGTGCTGATAGGAGATGTGGAAGATGCCATCTGCCTTCATGCC
TTCTACTATGCCATATGGAAACGATATGGTGCCCTCCCTGAGAGATATAACTGGCAGCTG
CAGGCCCCTGACGTTCTCTTCTACCCACTGAGACCAGAGTTAGTGGAATCCACATATCTC
CTCTACCAGGCAACCAAGAATCCCTTCTACCTCCATGTAGGAATGGATATTCTGCAGAGT
CTGGAAAAGTACACAAAAGTCAAGTGTGGGTACGCCACGCTGCATCACGTCATTGACAAG
TCCACAGAAGACCGGATGGAGAGCTTCTTTCTCAGTGAGACCTGTAAATATTTGTATCTG
CTGTTTGATGAAGACAATCCAGTACACAAGTCTGGAACCAGATACATGTTCACAACAGAG
GGACACATTGTATCTGTGGATGAGCATCTTCGGGAATTGCCATGGAAGGAATTCTTCTCT
GAAGAGGGAGGGCAGGACCAAGGGGGAAAGTCTGTGCACAGGCCGAAACCTCATGAGTTA
AAAGTCATCAACTCCAGCTCCAACTGCAATCGTGTACCTGATGAGAGGAGGTACTCCCTG
CCCTTAAAGAGCATCTACATGCGACAGATTGACCAGATGGTTGGTTTGATT

ORF Protein

Cloned ORF protein sequence for pF1KSDA0212 Download

>KIAA0212  657 aa
MQWRALVLGLVLLRLGLHGVLWLVFGLGPSMGFYQRFPLSFGFQRLRSPDGPASPTSGPV
GRPGGVSGPSWLQPPGTGAAQSPRKAPRRPGPGMCGPANWGYVLGGRGRGPDEYEKRYSG
AFPPQLRAQMRDLARGMFVFGYDNYMAHAFPQDELNPIHCRGRGPDRGDPSNLNINDVLG
NYSLTLVDALDTLAIMGNSSEFQKAVKLVINTVSFDKDSTVQVFEATIRVLGSLLSAHRI
ITDSKQPFGDMTIKDYDNELLYMAHDLAVRLLPAFENTKTGIPYPRVNLKTGVPPDTNNE
TCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNVVNIQTGHWVG
KQSGLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQNYLRRGREACNEGEGDPPLY
VNVNMFSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERYNWQL
QAPDVLFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDK
STEDRMESFFLSETCKYLYLLFDEDNPVHKSGTRYMFTTEGHIVSVDEHLRELPWKEFFS
EEGGQDQGGKSVHRPKPHELKVINSSSNCNRVPDERRYSLPLKSIYMRQIDQMVGLI

ORF+Vector

Nucleotide Sequence (with vector) for pF1KSDA0212 Download

>pF1KSDA0212  5108 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCTTTCGAAGGAGAT
AGAACCATGCAATGGCGAGCGCTCGTCCTGGGGCTGGTGCTCCTCCGGCTTGGCCTCCAT
GGAGTATTGTGGCTCGTCTTCGGGCTGGGGCCCAGCATGGGCTTCTACCAGCGCTTTCCG
CTCAGCTTCGGCTTCCAGCGTCTGAGGAGCCCCGACGGCCCCGCGTCGCCCACCTCGGGG
CCCGTGGGCCGGCCTGGGGGGGTATCCGGGCCGTCGTGGCTGCAGCCGCCGGGGACCGGG
GCAGCGCAGAGCCCGCGCAAGGCTCCGCGGCGTCCTGGGCCGGGGATGTGCGGCCCAGCC
AACTGGGGCTACGTGCTGGGCGGCCGGGGCCGCGGCCCGGACGAGTACGAGAAGCGCTAC
AGCGGCGCCTTCCCTCCGCAGCTGCGTGCCCAGATGCGCGACCTGGCACGGGGCATGTTC
GTCTTTGGCTACGACAACTACATGGCTCACGCCTTCCCCCAGGACGAGCTCAACCCCATC
CACTGCCGCGGCCGTGGGCCCGACCGCGGGGACCCTTCAAATCTGAACATCAATGATGTA
CTAGGGAACTACTCATTGACTCTTGTTGATGCATTGGATACACTTGCAATAATGGGAAAT
TCATCCGAGTTCCAGAAAGCAGTCAAGTTAGTGATCAACACAGTTTCATTTGACAAAGAT
TCCACCGTCCAAGTCTTTGAGGCCACGATAAGGGTCCTGGGAAGCCTCCTTTCTGCTCAC
AGAATAATAACTGACTCCAAGCAGCCCTTTGGTGACATGACAATTAAGGACTATGATAAT
GAGTTGTTATACATGGCCCATGACCTGGCGGTGCGGCTCCTCCCTGCTTTTGAAAACACC
AAGACAGGGATTCCATATCCTCGGGTGAATCTAAAGACAGGAGTTCCTCCTGACACCAAT
AATGAGACATGCACAGCGGGAGCCGGTTCCCTCCTGGTGGAATTTGGGATTCTGAGTCGA
CTCCTGGGGGACTCCACATTTGAGTGGGTGGCCAGACGAGCAGTGAAAGCCCTTTGGAAC
CTCCGGAGCAATGATACAGGATTACTAGGCAATGTCGTGAACATTCAGACGGGCCACTGG
GTTGGAAAGCAGAGTGGCCTGGGTGCCGGGCTGGACTCCTTCTATGAATACCTCTTGAAA
TCTTACATTCTCTTTGGAGAAAAAGAAGACCTAGAAATGTTTAATGCTGCATATCAGAGT
ATTCAGAACTACTTAAGAAGAGGGCGGGAAGCCTGCAATGAAGGAGAAGGAGACCCTCCA
CTCTATGTCAACGTGAACATGTTCAGTGGGCAGCTGATGAACACCTGGATTGACTCTCTG
CAGGCCTTTTTCCCTGGACTGCAGGTGCTGATAGGAGATGTGGAAGATGCCATCTGCCTT
CATGCCTTCTACTATGCCATATGGAAACGATATGGTGCCCTCCCTGAGAGATATAACTGG
CAGCTGCAGGCCCCTGACGTTCTCTTCTACCCACTGAGACCAGAGTTAGTGGAATCCACA
TATCTCCTCTACCAGGCAACCAAGAATCCCTTCTACCTCCATGTAGGAATGGATATTCTG
CAGAGTCTGGAAAAGTACACAAAAGTCAAGTGTGGGTACGCCACGCTGCATCACGTCATT
GACAAGTCCACAGAAGACCGGATGGAGAGCTTCTTTCTCAGTGAGACCTGTAAATATTTG
TATCTGCTGTTTGATGAAGACAATCCAGTACACAAGTCTGGAACCAGATACATGTTCACA
ACAGAGGGACACATTGTATCTGTGGATGAGCATCTTCGGGAATTGCCATGGAAGGAATTC
TTCTCTGAAGAGGGAGGGCAGGACCAAGGGGGAAAGTCTGTGCACAGGCCGAAACCTCAT
GAGTTAAAAGTCATCAACTCCAGCTCCAACTGCAATCGTGTACCTGATGAGAGGAGGTAC
TCCCTGCCCTTAAAGAGCATCTACATGCGACAGATTGACCAGATGGTTGGTTTGATTTAC
GTAGTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCAT
GCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGC
TGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCT
GAAAGGAGGAACTATATCCGGTTCGCTTGCTGTCCATAAAACCGCCCAGTCTAGCTATCG
CCATGTAAGCCCACTGCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGTCCA
GATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTA
CGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGAGCTTCA
AAAGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGG
ATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACA
GGATGACGGTCGTTTCGCATGCTTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCT
TGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCC
GCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCC
GGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGC
GTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTG
GGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCC
ATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGAC
CACCAAGCGAAACATCGCATCGAGCGAGCACGCACTCGGATGGAAGCCGGTCTTGTCGAT
CAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTC
AAGGCGCGTATGCCGGATGGTGAGGATCTCGTCGTGACTCATGGCGATGCCTGCTTGCCG
AATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTG
GCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGC
GAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATC
GCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCG
ACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGA
ATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCG
TAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAA
AAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTT
TCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCT
GTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCT
CAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCC
CGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTT
ATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGC
TACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTAT
CTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAA
ACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAA
AAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGA
AAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCT
TTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACC
ATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTG
CATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTT
TGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTA
CAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACC
GCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTTTTCAGCCTGATACA
GATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGC
GGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAG
TGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTC
AGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTA
GGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGG
CAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATG
GCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCAT