Restriction map for pF1KSDA0212
##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AhdI        
AlwI        AocI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbrPI       BbvCI       BcgI        BglII       
BlnI        BsgI        BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstYI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      DrdI        Eam1105I    Eco81I      
EcoO65I     EcoRV       FspI        HindIII     HpaI        
KpnI        MfeI        MflI        MluI        MunI        
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SwaI        
Tth111I     XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI          950  GTCGAC               GT^[AC][GT]AC
AccIII       1016  TCCGGA               T^CCGGA
AflII        1206  CTTAAG               C^TTAAG
Alw44I       1834  GTGCAC               G^TGCAC
ApaLI        1834  GTGCAC               G^TGCAC
BalI          983  TGGCCA               TGG^CCA
BbeI          358  GGCGCC               GGCGC^C
BclI          626  TGATCA               T^GATCA
BfrI         1206  CTTAAG               C^TTAAG
BlpI          114  GCTCAGC              GC^T[ACGT]AGC
BpmI         1872  CTCCAG               CTGGAG(16/14)
Bpu1102I      114  GCTCAGC              GC^T[ACGT]AGC
BsaAI         304  TACGTG               [CT]AC^GT[GA]
BsaBI        1759  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        1016  TCCGGA               T^CCGGA
BspEI        1016  TCCGGA               T^CCGGA
BspHI        1851  TCATGA               T^CATGA
BspMI        1335  GCAGGT               ACCTGC(4/8)
BsrBI         112  CCGCTC               CCGCTC(-3/-3)
CelII         114  GCTCAGC              GC^T[ACGT]AGC
DpnII         627  GATC                 ^GATC
EcoNI        1500  CCTCTACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1789  GAATTC               G^AATTC
EcoT22I       563  ATGCAT               ATGCA^T
FbaI          626  TGATCA               T^GATCA
FseI          181  GGCCGGCC             GGCCGG^CC
KasI          358  GGCGCC               G^GCGCC
MaeIII        746  GTGAC                ^GT[ACGT]AC
MamI         1759  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          627  GATC                 ^GATC
MluNI         983  TGGCCA               TGG^CCA
MroI         1016  TCCGGA               T^CCGGA
MscI          983  TGGCCA               TGG^CCA
NaeI          182  GCCGGC               GCC^GGC
NarI          358  GGCGCC               GG^CGCC
NdeI         1392  CATATG               CA^TATG
NdeII         627  GATC                 ^GATC
NgoMIV        182  GCCGGC               G^CCGGC
NsiI          563  ATGCAT               ATGCA^T
PflMI         792  CCATGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI         1851  TCATGA               T^CATGA
SacI          460  GAGCTC               GAGCT^C
SalI          950  GTCGAC               G^TCGAC
Sau3AI        627  GATC                 ^GATC
SfoI          358  GGCGCC               GGC^GCC
SmlI         1206  CTTAAG               C^T[CT][GA]AG
SspI         1667  AATATT               AAT^ATT
StuI         1316  AGGCCT               AGG^CCT
TaqI          951  TCGA                 T^CGA
Tsp45I        746  GTGAC                ^GT[GC]AC
TthHB8I       951  TCGA                 T^CGA
Van91I        792  CCATGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcyI          263  GGCGTC               G[GA]^CG[CT]C
              358  GGCGCC              
AflIII       1271  ACATGT               A^C[GA][CT]GT
             1724  ACATGT              
AvaI          168  CTCGGG               C^[CT]CG[GA]G
              854  CTCGGG              
BciVI         570  GGATAC               GTATCC(6/5)
              196  GTATCC              
BglI          143  GCCCCGACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              204  GCCGTCGTGGC         
BsaHI         263  GGCGTC               G[GA]^CG[CT]C
              358  GGCGCC              
BsaWI        1016  TCCGGA               [AT]^CCGG[AT]
             1631  ACCGGA              
BsoBI         168  CTCGGG               C^[CT]CG[GA]G
              854  CTCGGG              
BspLU11I     1271  ACATGT               A^CATGT
             1724  ACATGT              
BsrFI         182  GCCGGC               [GA]^CCGG[CT]
              916  GCCGGT              
Cfr10I        182  GCCGGC               [GA]^CCGG[CT]
              916  GCCGGT              
EagI          315  CGGCCG               C^GGCCG
              483  CGGCCG              
EclXI         315  CGGCCG               C^GGCCG
              483  CGGCCG              
Eco52I        315  CGGCCG               C^GGCCG
              483  CGGCCG              
Hin1I         263  GGCGTC               G[GA]^CG[CT]C
              358  GGCGCC              
HincII        950  GTCGAC               GT[CT]^[GA]AC
             1261  GTCAAC              
HindII        950  GTCGAC               GT[CT]^[GA]AC
             1261  GTCAAC              
HphI          745  GGTGA                GGTGA(8/7)
              858  GGTGA               
MslI          971  CATTTGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1932  CATCTACATG          
NcoI           51  CCATGG               C^CATGG
             1780  CCATGG              
PpuMI         503  GGGACCC              [GA]G^G[AT]CC[CT]
              686  GGGTCCT             


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwNI       Aor51HI     ApaI        ApoI        Asp700I     
AspHI       AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpuAI       
BsaI        BsaJI       BseRI       BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       Cac8I       CfoI        Cfr13I      
DdeI        DraII       DsaI        EaeI        EarI        
Eco47III    EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
ItaI        Ksp632I     KspI        MaeI        MaeII       
MboII       MnlI        MseI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NlaIII      
NlaIV       NspI        PleI        PstI        PvuII       
RsaI        SacII       Sau96I      ScrFI       SfaNI       
SfcI        StyI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       XmnI        
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