Gene/Protein Characteristic Table for KIAA0212
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Product ID ORK00461
Accession No D86967
Description ER degradation enhancer, mannosidase alpha-like 1
Clone name ha02602
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (6072 bp)
Predicted protein sequence (666 aa)
Flexi ORF Clone FXC00461
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0212 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 6072 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 4040 bp
Genome contig ID gi89161205f_5104433
PolyA signal sequence
(ATTAAA,-21)
+----*----+----*----+----*----+----
TATGAGTAATTTTTATTAAAAGATTTCTTTTTTTG
Flanking genome sequence
(132211 - 132260)
----+----*----+----*----+----*----+----*----+----*
AGTTGTCATCTTGTTTCTTTTTTACATTCTCATGTACATTACATTACATG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 3 f 5204433 5236642 12 99.3 Perfect prediction
Features of the protein sequence
Description

Length: 666 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q92611 0 100.0 ER degradation-...
Homo sapiens
XP_001098603 0 98.5 ER degradation ...
Macaca mulatta
AAI51306 0 95.1 EDEM1 protein [...
Bos taurus
Q925U4 0 92.3 ER degradation-...
Mus musculus
BAE30594 0 92.1 unnamed protein...
Mus musculus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR001382 146 166 PR00747 Glycoside hydrolase
IPR001382 192 206 PR00747 Glycoside hydrolase
IPR001382 229 247 PR00747 Glycoside hydrolase
IPR001382 283 302 PR00747 Glycoside hydrolase
IPR001382 375 392 PR00747 Glycoside hydrolase
IPR001382 443 459 PR00747 Glycoside hydrolase
IPR001382 498 522 PR00747 Glycoside hydrolase
IPR001382 557 577 PR00747 Glycoside hydrolase
HMMPfam IPR001382 146 595 PF01532 Glycoside hydrolase

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 15 LVLGLVLLRLGLHGVLWLVFGLG 37 PRIMARY 23
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 3
Experimental conditions
Panel name Genebridge 4
Primer_f TACCCTCTTCCTTCTTTCTGC
Primer_r TTTCCGTAAGTTTTCCCAGAG
PCR product length 171 bp
PCR conditions 95 °C15 sec66 °C60 sec30 cycles
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