EIF4A3 ORF clone FXC00412

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Flexi ORF Clone FXC00412

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Clone info.

Species

human

Clone Name

pF1KSDA0111 Sequence (with vector) Restriction Map

Product ID

FXC00412

HaloTag Clone PID

FHC00412

ORF Sequence

DNA sequence (1233 bp)
Protein sequence (411 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383751]

Gene

KIAA0111

Symbol

EIF4A3

Alias : DDX48, Fal1, MUK34, NMP265, NUK34, RCPS, eIF-4A-III, eIF4A-III, eIF4AIII

Description

eukaryotic translation initiation factor 4A3

Original Clone ID

ha00659

Features of the cloned ORF sequence

Description

Length: 1233 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 411 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11767.1 3.4e-179 100.0 EIF4A3 CCDS3282.1 2.3e-119 71.4 EIF4A2 CCDS11113.1 1.1e-118 66.7 EIF4A1 CCDS58511.1 5.3e-93 64.7 EIF4A1 CCDS44751.1 7.9e-60 36.8 DDX6 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011545 63 225 PF00270 DNA/RNA helicase IPR001650 296 372 PF00271 Helicase HMMSmart IPR014001 57 254 SM00487 DEAD-like helicase IPR001650 291 372 SM00490 Helicase ProfileScan IPR014014 38 66 PS51195 RNA helicase IPR014001 69 239 PS51192 DEAD-like helicase IPR001650 250 411 PS51194 Helicase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055555 411 268305,608546 100.0 100.0 XP_011523824 390 268305,608546 94.9 100.0 NP_001958 407 601102 71.4 91.7 NP_001407 406 602641 66.7 98.5 NP_001191439 347 602641 64.7 81.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KSDA0111 Download

>KIAA0111  1233 bp
ATGGCGACCACGGCCACGATGGCGACCTCGGGCTCGGCGCGAAAGCGGCTGCTCAAAGAG
GAAGACATGACTAAAGTGGAATTCGAGACCAGCGAGGAGGTGGATGTGACCCCCACGTTC
GACACCATGGGCCTGCGGGAGGACCTGCTGCGGGGCATCTACGCTTACGGTTTTGAAAAA
CCATCAGCAATCCAGCAACGAGCAATCAAGCAGATCATCAAAGGGAGAGATGTCATCGCA
CAGTCTCAGTCCGGCACAGGAAAAACAGCCACCTTCAGTATCTCAGTCCTCCAGTGTTTG
GATATTCAGGTTCGTGAAACTCAAGCTTTGATCTTGGCTCCCACAAGAGAGTTGGCTGTG
CAGATCCAGAAGGGGCTGCTTGCTCTCGGTGACTACATGAATGTCCAGTGCCATGCCTGC
ATTGGAGGCACCAATGTTGGCGAGGACATCAGGAAGCTGGATTACGGACAGCATGTTGTC
GCGGGCACTCCAGGGCGTGTTTTTGATATGATTCGTCGCAGAAGCCTAAGGACACGTGCT
ATCAAAATGTTGGTTTTGGATGAAGCTGATGAAATGTTGAATAAAGGTTTCAAAGAGCAG
ATTTACGATGTATACAGGTACCTGCCTCCAGCCACACAGGTGGTTCTCATCAGTGCCACG
CTGCCACACGAGATTCTGGAGATGACCAACAAGTTCATGACCGACCCAATCCGCATCTTG
GTGAAACGTGATGAATTGACTCTGGAAGGCATCAAGCAATTTTTCGTGGCAGTGGAGAGG
GAAGAGTGGAAATTTGACACTCTGTGTGACCTCTACGACACACTGACCATCACTCAGGCG
GTCATCTTCTGCAACACCAAAAGAAAGGTGGACTGGCTGACGGAGAAAATGAGGGAAGCC
AACTTCACTGTATCCTCAATGCATGGAGACATGCCCCAGAAAGAGCGGGAGTCCATCATG
AAGGAGTTCCGGTCGGGCGCCAGCCGAGTGCTTATTTCTACAGATGTCTGGGCCAGGGGG
TTGGATGTCCCTCAGGTGTCCCTCATCATTAACTATGATCTCCCTAATAACAGAGAATTG
TACATACACAGAATTGGGAGATCAGGTCGATACGGCCGGAAGGGTGTGGCCATTAACTTT
GTAAAGAATGACGACATCCGCATCCTCAGAGATATCGAGCAGTACTATTCCACTCAGATT
GATGAGATGCCGATGAACGTTGCTGATCTTATC

ORF Protein

Cloned ORF protein sequence for pF1KSDA0111 Download

>KIAA0111  411 aa
MATTATMATSGSARKRLLKEEDMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEK
PSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAV
QIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRA
IKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQVVLISATLPHEILEMTNKFMTDPIRIL
VKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREA
NFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNREL
YIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI

ORF+Vector

Nucleotide Sequence (with vector) for pF1KSDA0111 Download

>pF1KSDA0111  4370 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCTTTCGAAGGAGAT
AGAACCATGGCGACCACGGCCACGATGGCGACCTCGGGCTCGGCGCGAAAGCGGCTGCTC
AAAGAGGAAGACATGACTAAAGTGGAATTCGAGACCAGCGAGGAGGTGGATGTGACCCCC
ACGTTCGACACCATGGGCCTGCGGGAGGACCTGCTGCGGGGCATCTACGCTTACGGTTTT
GAAAAACCATCAGCAATCCAGCAACGAGCAATCAAGCAGATCATCAAAGGGAGAGATGTC
ATCGCACAGTCTCAGTCCGGCACAGGAAAAACAGCCACCTTCAGTATCTCAGTCCTCCAG
TGTTTGGATATTCAGGTTCGTGAAACTCAAGCTTTGATCTTGGCTCCCACAAGAGAGTTG
GCTGTGCAGATCCAGAAGGGGCTGCTTGCTCTCGGTGACTACATGAATGTCCAGTGCCAT
GCCTGCATTGGAGGCACCAATGTTGGCGAGGACATCAGGAAGCTGGATTACGGACAGCAT
GTTGTCGCGGGCACTCCAGGGCGTGTTTTTGATATGATTCGTCGCAGAAGCCTAAGGACA
CGTGCTATCAAAATGTTGGTTTTGGATGAAGCTGATGAAATGTTGAATAAAGGTTTCAAA
GAGCAGATTTACGATGTATACAGGTACCTGCCTCCAGCCACACAGGTGGTTCTCATCAGT
GCCACGCTGCCACACGAGATTCTGGAGATGACCAACAAGTTCATGACCGACCCAATCCGC
ATCTTGGTGAAACGTGATGAATTGACTCTGGAAGGCATCAAGCAATTTTTCGTGGCAGTG
GAGAGGGAAGAGTGGAAATTTGACACTCTGTGTGACCTCTACGACACACTGACCATCACT
CAGGCGGTCATCTTCTGCAACACCAAAAGAAAGGTGGACTGGCTGACGGAGAAAATGAGG
GAAGCCAACTTCACTGTATCCTCAATGCATGGAGACATGCCCCAGAAAGAGCGGGAGTCC
ATCATGAAGGAGTTCCGGTCGGGCGCCAGCCGAGTGCTTATTTCTACAGATGTCTGGGCC
AGGGGGTTGGATGTCCCTCAGGTGTCCCTCATCATTAACTATGATCTCCCTAATAACAGA
GAATTGTACATACACAGAATTGGGAGATCAGGTCGATACGGCCGGAAGGGTGTGGCCATT
AACTTTGTAAAGAATGACGACATCCGCATCCTCAGAGATATCGAGCAGTACTATTCCACT
CAGATTGATGAGATGCCGATGAACGTTGCTGATCTTATCTACGTAGTTTAAACGAATTCG
AGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGC
TAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATA
ACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATC
CGGTTCGCTTGCTGTCCATAAAACCGCCCAGTCTAGCTATCGCCATGTAAGCCCACTGCA
AGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGAC
ATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTGTTCCGCTTCCTTTA
GCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGAGCTTCAAAAGAATTGCCAGCTGGG
GCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCA
AGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGACGGTCGTTTCGC
ATGCTTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTC
GGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCA
GCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTG
CAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTG
CTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAG
GATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATG
CGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGC
ATCGAGCGAGCACGCACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAA
GAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGTATGCCGGAT
GGTGAGGATCTCGTCGTGACTCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAAT
GGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGAC
ATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTC
CTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTT
GACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACC
GGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGG
AAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT
GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCA
GAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCT
CGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTC
GGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGT
TCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC
CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGC
CACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTG
GTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCC
AGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAG
CGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGA
TCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGAT
TTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATAC
AGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTC
AGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGT
TAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTG
TCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGT
AAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGA
TGCGTCCGGCGTAGAGGATCGAGATCTTTTCAGCCTGATACAGATTAAATCAGAACGCAG
AAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCC
CATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGC
GAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCT
TTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAG
CGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAA
CTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTAC
AAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCAT