Restriction map for pF1KSDA0111
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       AccIII      AflII       AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        AspEI       AspHI       
AspI        AviII       AvrII       BamHI       BbvCI       
BcgI        BclI        BcnI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BseAI       BsiHKAI     BsiWI       BsmBI       BsmI        
BspDI       BspEI       BspLU11I    BsrDI       BsrFI       
BssHII      BstAPI      BstBI       BstEII      BstPI       
CelII       Cfr10I      ClaI        CpoI        DraI        
DrdI        Eam1105I    Eco47III    EcoO65I     FbaI        
FseI        FspI        HgaI        HincII      HindII      
HpaI        KspI        MaeI        MamI        MfeI        
MluI        MroI        MspA1I      MunI        NaeI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshAI       PshBI       PstI        
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   70 enzymes) #
###################################

Acc65I        617  GGTACC               G^GTACC
AccI          610  GTATAC               GT^[AC][GT]AC
AclI         1216  AACGTT               AA^CGTT
AcyI          976  GGCGCC               G[GA]^CG[CT]C
AflIII        532  ACACGT               A^C[GA][CT]GT
AlwI          363  GATCC                GGATC(4/5)
Asp700I       960  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       617  GGTACC               G^GTACC
AvaI           27  CTCGGG               C^[CT]CG[GA]G
AvaII         141  GGACC                G^G[AT]CC
BalI         1127  TGGCCA               TGG^CCA
BanII          30  GGGCTC               G[GA]GC[CT]^C
BbeI          976  GGCGCC               GGCGC^C
BbrPI         533  CACGTG               CAC^GTG
BbsI           61  GAAGAC               GAAGAC(2/6)
BciVI         910  GTATCC               GTATCC(6/5)
BglI           13  GCCACGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI          61  GAAGAC               GAAGAC(2/6)
BsaAI         533  CACGTG               [CT]AC^GT[GA]
BsaHI         976  GGCGCC               G[GA]^CG[CT]C
BsaI           85  GAGACC               GGTCTC(1/5)
BsaWI         968  TCCGGT               [AT]^CCGG[AT]
BseRI          94  GAGGAG               GAGGAG(10/8)
BsgI          358  GTGCAG               GTGCAG(16/14)
BsoBI          27  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     1079  TGTACA               T^GTACA
BsrBI         943  GAGCGG               CCGCTC(-3/-3)
BsrGI        1079  TGTACA               T^GTACA
BssSI         667  CACGAG               CACGAG(-5/-1)
Bst1107I      610  GTATAC               GTA^TAC
BstYI         362  AGATCC               [GA]^GATC[CT]
BstZ17I       610  GTATAC               GTA^TAC
DraII         140  AGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII        798  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         1113  CGGCCG               C^GGCCG
EarI          781  GAAGAG               CTCTTC(1/4)
EclXI        1113  CGGCCG               C^GGCCG
Eco52I       1113  CGGCCG               C^GGCCG
EcoNI         132  CCTGCGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      140  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI          79  GAATTC               G^AATTC
EcoRV        1171  GATATC               GAT^ATC
EcoT14I       125  CCATGG               C^C[AT][AT]GG
EcoT22I       919  ATGCAT               ATGCA^T
HaeII         976  GGCGCC               [GA]GCGC^[CT]
Hin1I         976  GGCGCC               G[GA]^CG[CT]C
HindIII       323  AAGCTT               A^AGCTT
KasI          976  GGCGCC               G^GCGCC
KpnI          617  GGTACC               GGTAC^C
Ksp632I       781  GAAGAG               CTCTTC(1/4)
MflI          362  AGATCC               [GA]^GATC[CT]
MluNI        1127  TGGCCA               TGG^CCA
MscI         1127  TGGCCA               TGG^CCA
NarI          976  GGCGCC               GG^CGCC
NcoI          125  CCATGG               C^CATGG
NsiI          919  ATGCAT               ATGCA^T
PflMI         291  CCAGTGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         533  CACGTG               CAC^GTG
PmlI          533  CACGTG               CAC^GTG
PpuMI         140  AGGACCT              [GA]G^G[AT]CC[CT]
Psp1406I     1216  AACGTT               AA^CGTT
ScaI         1181  AGTACT               AGT^ACT
SfcI          998  CTACAG               C^T[GA][CT]AG
SfoI          976  GGCGCC               GGC^GCC
SmlI          320  CTCAAG               C^T[CT][GA]AG
SspBI        1079  TGTACA               T^GTACA
StyI          125  CCATGG               C^C[AT][AT]GG
Van91I        291  CCAGTGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         362  AGATCC               [GA]^GATC[CT]
XmnI          960  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccII          38  CGCG                 CG^CG
              480  CGCG                
AcsI           79  GAATTC               [GA]^AATT[CT]
              790  AAATTT              
AocI          525  CCTAAGG              CC^T[ACGT]AGG
             1030  CCTCAGG             
ApoI           79  GAATTC               [GA]^AATT[CT]
              790  AAATTT              
BmyI           30  GGGCTC               G[AGT]GC[ACT]^C
              483  GGGCAC              
BsiEI         970  CGGTCG               CG[GA][CT]^CG
             1113  CGGCCG              
BsmFI        1027  GTCCC                GGGAC(10/14)
             1038  GTCCC               
Bsp1286I       30  GGGCTC               G[AGT]GC[ACT]^C
              483  GGGCAC              
BspHI         695  TCATGA               T^CATGA
              956  TCATGA              
BspMI         143  ACCTGC               ACCTGC(4/8)
              620  ACCTGC              
BssKI         490  CCAGG                ^CC[ACGT]GG
             1013  CCAGG               
BstNI         490  CCAGG                CC^[AT]GG
             1013  CCAGG               
BstUI          38  CGCG                 CG^CG
              480  CGCG                
BstXI         632  CCACACAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1013  CCAGGGGGTTGG        
Bsu36I        525  CCTAAGG              CC^T[ACGT]AGG
             1030  CCTCAGG             
CfoI           37  GCGC                 GCG^C
              977  GCGC                
DsaI            8  CCACGG               C^C[GA][CT]GG
              125  CCATGG              
Eco81I        525  CCTAAGG              CC^T[ACGT]AGG
             1030  CCTCAGG             
EcoRII        490  CCAGG                ^CC[AT]GG
             1013  CCAGG               
HhaI           37  GCGC                 GCG^C
              977  GCGC                
HinP1I         37  GCGC                 G^CGC
              977  GCGC                
HphI          388  GGTGA                GGTGA(8/7)
              720  GGTGA               
MseI         1049  TTAA                 T^TAA
             1133  TTAA                
MslI          633  CACACAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              714  CATCTTGGTG          
MvaI          490  CCAGG                CC^[AT]GG
             1013  CCAGG               
MvnI           38  CGCG                 CG^CG
              480  CGCG                
NspI          471  GCATGT               [GA]CATG^[CT]
              929  ACATGC              
PleI          738  GACTC                GAGTC(4/5)
              949  GAGTC               
RcaI          695  TCATGA               T^CATGA
              956  TCATGA              
ScrFI         490  CCAGG                CC^[ACGT]GG
             1013  CCAGG               
TfiI          510  GATTC                G^A[AT]TC
              672  GATTC               
Tru9I        1049  TTAA                 T^TAA
             1133  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AfaI        AluI        BanI        BbvI        
BpmI        BsaJI       BsiYI       BslI        BsmAI       
BsrI        BstF5I      Cac8I       Cfr13I      DdeI        
DpnII       EaeI        FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MspI        MwoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      SfaNI       TaqI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        
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