NHLRC1 ORF clone FHC22455

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC22455

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Clone info.

Species

human

Clone Name

pFN21AE3757 Sequence (with vector) Restriction Map

Product ID

FHC22455

ORF Sequence

DNA sequence (1185 bp)
Protein sequence (395 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3757

Symbol

NHLRC1

Alias : EPM2A, EPM2B, MALIN, bA204B7.2

Description

NHL repeat containing E3 ubiquitin protein ligase 1

Original Clone ID

cp93757

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1185 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 395 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4542.1 5.8e-139 100.0 NHLRC1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMSmart IPR001841 26 71 SM00184 Zinc finger ProfileScan IPR001841 26 72 PS50089 Zinc finger IPR013017 113 157 PS51125 NHL repeat IPR013017 161 204 PS51125 NHL repeat IPR013017 212 245 PS51125 NHL repeat IPR013017 248 300 PS51125 NHL repeat IPR013017 301 349 PS51125 NHL repeat IPR013017 350 393 PS51125 NHL repeat
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_940988 395 254780,608072 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3757 Download

>KIEE3757  1185 bp
ATGGCGGCCGAAGCCTCGGAGAGCGGGCCAGCGCTGCATGAGCTCATGCGCGAGGCGGAG
ATCAGCCTGCTCGAGTGCAAGGTGTGCTTTGAGAAGTTTGGCCACCGGCAGCAGCGGCGC
CCGCGCAACCTGTCCTGCGGCCACGTGGTCTGCCTGGCCTGCGTGGCCGCCCTGGCGCAC
CCGCGCACTCTGGCCCTCGAGTGCCCATTCTGCAGGCGAGCTTGCCGGGGCTGCGACACC
AGCGACTGCCTGCCGGTGCTGCACCTCATAGAGCTCCTGGGCTCAGCGCTTCGCCAGTCC
CCGGCCGCCCATCGCGCCGCCCCCAGCGCCCCCGGAGCCCTCACCTGCCACCACACCTTC
GGCGGCTGGGGGACCCTGGTCAACCCCACCGGACTGGCGCTTTGTCCCAAGACGGGGCGT
GTCGTGGTGGTGCACGACGGCAGGAGGCGTGTCAAGATTTTTGACTCAGGGGGAGGATGC
GCGCATCAGTTTGGAGAGAAGGGGGACGCTGCCCAAGACATTAGGTACCCTGTGGATGTC
ACCATCACCAACGACTGCCATGTGGTTGTCACTGACGCCGGCGATCGTTCCATCAAAGTG
TTTGATTTTTTTGGCCAGATCAAGCTTGTCATTGGAGGCCAATTCTCCTTACCTTGGGGT
GTGGAGACCACCCCTCAGAATGGGATTGTGGTAACTGATGCGGAGGCAGGGTCCCTGCAC
CTCCTGGACGTCGACTTCGCGGAAGGGGTCCTTCGGAGAACTGAAAGGTTGCAAGCTCAT
CTGTGCAATCCCCGAGGGGTGGCAGTGTCTTGGCTCACCGGGGCCATTGCGGTCCTGGAG
CACCCCCTGGCCCTGGGGACTGGGGTTTGCAGCACCAGGGTGAAAGTGTTTAGCTCAAGT
ATGCAGCTTGTCGGCCAAGTGGATACCTTTGGGCTGAGCCTCTACTTTCCCTCCAAAATA
ACTGCCTCCGCTGTGACCTTTGATCACCAGGGAAATGTGATTGTTGCAGATACATCTGGT
CCAGCTATCCTTTGCTTAGGAAAACCTGAGGAGTTTCCAGTACCGAAGCCCATGGTCACT
CATGGTCTTTCGCATCCTGTGGCTCTTACCTTCACCAAGGAGAATTCTCTTCTTGTGCTG
GACACAGCATCTCATTCTATAAAAGTCTATAAAGTTGACTGGGGG

ORF Protein

Cloned ORF protein sequence for pFN21AE3757 Download

>KIEE3757  395 aa
MAAEASESGPALHELMREAEISLLECKVCFEKFGHRQQRRPRNLSCGHVVCLACVAALAH
PRTLALECPFCRRACRGCDTSDCLPVLHLIELLGSALRQSPAAHRAAPSAPGALTCHHTF
GGWGTLVNPTGLALCPKTGRVVVVHDGRRRVKIFDSGGGCAHQFGEKGDAAQDIRYPVDV
TITNDCHVVVTDAGDRSIKVFDFFGQIKLVIGGQFSLPWGVETTPQNGIVVTDAEAGSLH
LLDVDFAEGVLRRTERLQAHLCNPRGVAVSWLTGAIAVLEHPLALGTGVCSTRVKVFSSS
MQLVGQVDTFGLSLYFPSKITASAVTFDHQGNVIVADTSGPAILCLGKPEEFPVPKPMVT
HGLSHPVALTFTKENSLLVLDTASHSIKVYKVDWG

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3757 Download

>pFN21AE3757  5890 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGCCGAAGCCTCGGAGAGCGGGCCAGCGCT
GCATGAGCTCATGCGCGAGGCGGAGATCAGCCTGCTCGAGTGCAAGGTGTGCTTTGAGAA
GTTTGGCCACCGGCAGCAGCGGCGCCCGCGCAACCTGTCCTGCGGCCACGTGGTCTGCCT
GGCCTGCGTGGCCGCCCTGGCGCACCCGCGCACTCTGGCCCTCGAGTGCCCATTCTGCAG
GCGAGCTTGCCGGGGCTGCGACACCAGCGACTGCCTGCCGGTGCTGCACCTCATAGAGCT
CCTGGGCTCAGCGCTTCGCCAGTCCCCGGCCGCCCATCGCGCCGCCCCCAGCGCCCCCGG
AGCCCTCACCTGCCACCACACCTTCGGCGGCTGGGGGACCCTGGTCAACCCCACCGGACT
GGCGCTTTGTCCCAAGACGGGGCGTGTCGTGGTGGTGCACGACGGCAGGAGGCGTGTCAA
GATTTTTGACTCAGGGGGAGGATGCGCGCATCAGTTTGGAGAGAAGGGGGACGCTGCCCA
AGACATTAGGTACCCTGTGGATGTCACCATCACCAACGACTGCCATGTGGTTGTCACTGA
CGCCGGCGATCGTTCCATCAAAGTGTTTGATTTTTTTGGCCAGATCAAGCTTGTCATTGG
AGGCCAATTCTCCTTACCTTGGGGTGTGGAGACCACCCCTCAGAATGGGATTGTGGTAAC
TGATGCGGAGGCAGGGTCCCTGCACCTCCTGGACGTCGACTTCGCGGAAGGGGTCCTTCG
GAGAACTGAAAGGTTGCAAGCTCATCTGTGCAATCCCCGAGGGGTGGCAGTGTCTTGGCT
CACCGGGGCCATTGCGGTCCTGGAGCACCCCCTGGCCCTGGGGACTGGGGTTTGCAGCAC
CAGGGTGAAAGTGTTTAGCTCAAGTATGCAGCTTGTCGGCCAAGTGGATACCTTTGGGCT
GAGCCTCTACTTTCCCTCCAAAATAACTGCCTCCGCTGTGACCTTTGATCACCAGGGAAA
TGTGATTGTTGCAGATACATCTGGTCCAGCTATCCTTTGCTTAGGAAAACCTGAGGAGTT
TCCAGTACCGAAGCCCATGGTCACTCATGGTCTTTCGCATCCTGTGGCTCTTACCTTCAC
CAAGGAGAATTCTCTTCTTGTGCTGGACACAGCATCTCATTCTATAAAAGTCTATAAAGT
TGACTGGGGGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT