(Circular) (Six-base) MAPSORT of: pFN21AE3757.seq Check: 7588 from: 1 to: 5890 pFN21AE3757 5890 bp With 182 enzymes: SgfI * March 28, 2012 12:04 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2357 2357 Size: 5890 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2736 3700 278 Size: 53 83 186 936 1200 964 2468 Fragments arranged by size: 2468 1200 964 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2736 3233 1937 Size: 799 497 4594 Fragments arranged by size: 4594 799 497 Acc65I G'GTAC_C Cuts at: 2529 3211 2529 Size: 682 5208 AclI AA'CG_TT Cuts at: 1563 4017 4390 1563 Size: 2454 373 3063 Fragments arranged by size: 3063 2454 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3953 5337 5605 1632 Size: 2321 1384 268 1917 Fragments arranged by size: 2321 1917 1384 268 AfeI AGC'GCT Cuts at: 2037 2292 2037 Size: 255 5635 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5693 AflIII A'CryG_T Cuts at: 1203 4789 5766 1203 Size: 3586 977 1327 Fragments arranged by size: 3586 1327 977 AgeI A'CCGG_T Cuts at: 4721 4721 Size: 5890 AhdI GACnn_n'nnGTC Cuts at: 4619 4619 Size: 5890 AleI CACnn'nnGTG Cuts at: 1166 2882 1166 Size: 1716 4174 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5890 AlwNI CAG_nnn'CTG Cuts at: 2250 3633 5205 2250 Size: 1383 1572 2935 Fragments arranged by size: 2935 1572 1383 ApaLI G'TGCA_C Cuts at: 2435 3947 5103 2435 Size: 1512 1156 3222 Fragments arranged by size: 3222 1512 1156 ApoI r'AATT_y Cuts at: 1493 3127 3199 3425 3577 5686 1493 Size: 1634 72 226 152 2109 1697 Fragments arranged by size: 2109 1697 1634 226 152 72 AseI AT'TA_AT Cuts at: 160 1032 4444 160 Size: 872 3412 1606 Fragments arranged by size: 3412 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 AvaI C'yCGr_G Cuts at: 1870 1943 2075 2201 2796 3215 1870 Size: 73 132 126 595 419 4545 Fragments arranged by size: 4545 595 419 132 126 73 BamHI G'GATC_C Cuts at: 3220 3587 3220 Size: 367 5523 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2121 2529 3211 4666 618 Size: 325 217 627 334 408 682 1455 1842 Fragments arranged by size: 1842 1455 682 627 408 334 325 217 BanII G_rGCy'C Cuts at: 1391 2049 2280 2288 2344 3209 1391 Size: 658 231 8 56 865 4072 Fragments arranged by size: 4072 865 658 231 56 8 BbeI G_GCGC'C Cuts at: 2125 2125 Size: 5890 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4919 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4081 4115 1903 Size: 34 2144 34 3678 Fragments arranged by size: 3678 2144 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2920 3782 4992 2920 Size: 862 1210 3818 Fragments arranged by size: 3818 1210 862 BclI T'GATC_A Cuts at: 1548 2986 1548 Size: 1438 4452 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2168 2177 Size: 107 122 71 631 605 337 159 9 Cuts at: 2177 4501 136 Size: 2324 1525 Fragments arranged by size: 2324 1525 631 605 337 159 122 107 71 9 BglII A'GATC_T Cuts at: 5885 5885 Size: 5890 BlpI GC'TnA_GC Cuts at: 2287 2939 3307 2287 Size: 652 368 4870 Fragments arranged by size: 4870 652 368 Bme1580I G_kGCm'C Cuts at: 1790 2210 2439 3951 5107 1790 Size: 420 229 1512 1156 2573 Fragments arranged by size: 2573 1512 1156 420 229 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5890 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2874 3193 3621 Size: 416 53 83 399 24 1457 319 428 Cuts at: 3621 4579 442 Size: 958 1753 Fragments arranged by size: 1753 1457 958 428 416 399 319 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5890 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2861 4550 1239 Size: 1622 1689 2579 Fragments arranged by size: 2579 1689 1622 Bpu10I CC'TnA_GC Cuts at: 3040 3040 Size: 5890 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2884 4015 4880 5178 2884 Size: 1131 865 298 3596 Fragments arranged by size: 3596 1131 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2663 4553 882 Size: 617 1164 1890 2219 Fragments arranged by size: 2219 1890 1164 617 BsaAI yAC'GTr Cuts at: 493 1204 2149 3681 493 Size: 711 945 1532 2702 Fragments arranged by size: 2702 1532 945 711 BsaBI GATnn'nnATC Cuts at: 5884 5884 Size: 5890 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2122 2580 2733 3697 Size: 53 83 186 936 589 458 153 964 Cuts at: 3697 4079 4714 275 Size: 382 635 1451 Fragments arranged by size: 1451 964 936 635 589 458 382 186 153 83 53 BsaWI w'CCGG_w Cuts at: 2393 4323 4721 4995 5142 5553 2393 Size: 1930 398 274 147 411 2730 Fragments arranged by size: 2730 1930 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2699 2729 2939 2969 1178 Size: 30 165 30 1296 30 210 30 4099 Fragments arranged by size: 4099 1296 210 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3068 1186 Size: 1882 4008 BseYI C'CCAG_C Cuts at: 2327 2370 5093 5589 2327 Size: 43 2723 496 2628 Fragments arranged by size: 2723 2628 496 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2249 2705 1163 Size: 573 513 456 4348 Fragments arranged by size: 4348 573 513 456 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 2310 2590 3340 4101 5129 Size: 275 186 12 297 280 750 761 1028 Cuts at: 5129 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 750 297 280 275 186 12 BsiHKAI G_wGCw'C Cuts at: 2049 2280 2439 2847 3951 4036 5107 2049 Size: 231 159 408 1104 85 1071 2832 Fragments arranged by size: 2832 1104 1071 408 231 159 85 BsmI GAATG_Cn' Cuts at: 3401 3494 3401 Size: 93 5797 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2210 2280 2288 2344 2439 2847 Size: 399 259 161 70 8 56 95 408 Cuts at: 2847 3209 3951 4036 5107 1391 Size: 362 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 408 399 362 259 161 95 85 70 56 8 BspEI T'CCGG_A Cuts at: 5553 5553 Size: 5890 BspHI T'CATG_A Cuts at: 3779 5509 3779 Size: 1730 4160 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2357 3245 844 Size: 1513 888 3489 Fragments arranged by size: 3489 1513 888 BsrBI CCG'CTC Cuts at: 1394 1823 2028 3777 1394 Size: 429 205 1749 3507 Fragments arranged by size: 3507 1749 429 205 BsrDI GCAATG_nn' Cuts at: 65 1248 2829 4385 4559 65 Size: 1183 1581 1556 174 1396 Fragments arranged by size: 1581 1556 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2109 2257 2582 4534 4721 1700 Size: 110 299 148 325 1952 187 2869 Fragments arranged by size: 2869 1952 325 299 187 148 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5890 BssHII G'CGCG_C Cuts at: 1925 2484 1925 Size: 559 5331 BssSI C'ACGA_G Cuts at: 3950 4962 3950 Size: 1012 4878 BstAPI GCAn_nnn'nTGC Cuts at: 794 2088 794 Size: 1294 4596 BstBI TT'CG_AA Cuts at: 4694 4694 Size: 5890 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5890 BstXI CCAn_nnnn'nTGG Cuts at: 2398 2398 Size: 5890 BstYI r'GATC_y Cuts at: 1972 3220 3587 3972 3989 5441 5527 5539 5885 Size: 1248 367 385 17 1452 86 12 346 Cuts at: 5885 1972 Size: 1977 Fragments arranged by size: 1977 1452 1248 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3051 3051 Size: 5890 BtgI C'CryG_G Cuts at: 513 1065 2004 3075 513 Size: 552 939 1071 3328 Fragments arranged by size: 3328 1071 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2300 487 Size: 683 56 1074 4077 Fragments arranged by size: 4077 1074 683 56 BtsI GCAGTG_nn' Cuts at: 2814 3406 4200 4220 2814 Size: 592 794 20 4484 Fragments arranged by size: 4484 794 592 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5890 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5855 DraI TTT'AAA Cuts at: 3194 3194 Size: 5890 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4897 809 Size: 507 3581 1802 Fragments arranged by size: 3581 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2104 2143 Size: 54 1425 50 275 18 180 94 39 Cuts at: 2143 2169 2307 2617 2917 3337 4226 5855 8 Size: 26 138 310 300 420 889 1629 43 Fragments arranged by size: 1629 1425 889 420 310 300 275 180 138 94 54 50 43 39 26 18 EagI C'GGCC_G Cuts at: 1537 1812 2010 2307 3337 1537 Size: 275 198 297 1030 4090 Fragments arranged by size: 4090 1030 297 275 198 EarI CTCTTCn'nnn_ Cuts at: 1353 3138 3820 1353 Size: 1785 682 3423 Fragments arranged by size: 3423 1785 682 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2075 4477 4851 4997 387 Size: 1064 138 486 2402 374 146 1280 Fragments arranged by size: 2402 1280 1064 486 374 146 138 EcoICRI GAG'CTC Cuts at: 2047 2278 2047 Size: 231 5659 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2861 3953 4550 5337 5605 1239 Size: 393 1229 1092 597 787 268 1524 Fragments arranged by size: 1524 1229 1092 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2376 2715 2752 2376 Size: 339 37 5514 Fragments arranged by size: 5514 339 37 EcoRI G'AATT_C Cuts at: 3127 3199 3577 3127 Size: 72 378 5440 Fragments arranged by size: 5440 378 72 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2107 2771 2107 Size: 664 5226 FspI TGC'GCA Cuts at: 4396 4396 Size: 5890 HaeII r_GCGC'y Cuts at: 1678 2039 2125 2294 2334 2405 5037 1678 Size: 361 86 169 40 71 2632 2531 Fragments arranged by size: 2632 2531 361 169 86 71 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2662 2694 2699 2731 2971 Size: 32 135 32 1090 32 5 32 240 Cuts at: 2971 3003 3137 3169 4531 4563 4605 4637 1373 Size: 32 134 32 1362 32 42 32 2626 Fragments arranged by size: 2626 1362 1090 240 135 134 42 32 32 32 32 32 32 32 32 5 HincII GTy'rAC Cuts at: 669 1657 1938 2386 2737 3181 3234 3480 669 Size: 988 281 448 351 444 53 246 3079 Fragments arranged by size: 3079 988 448 444 351 281 246 53 HindIII A'AGCT_T Cuts at: 748 2627 748 Size: 1879 4011 HpaI GTT'AAC Cuts at: 3480 3480 Size: 5890 KasI G'GCGC_C Cuts at: 2121 2121 Size: 5890 KpnI G_GTAC'C Cuts at: 2533 3215 2533 Size: 682 5208 MfeI C'AATT_G Cuts at: 3489 3489 Size: 5890 MluI A'CGCG_T Cuts at: 5766 5766 Size: 5890 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5004 5188 1305 Size: 3699 184 2007 Fragments arranged by size: 3699 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2106 2619 10 Size: 54 1425 343 274 513 3281 Fragments arranged by size: 3281 1425 513 343 274 54 MslI CAynn'nnrTG Cuts at: 518 1166 2600 2882 3848 4207 4366 518 Size: 648 1434 282 966 359 159 2042 Fragments arranged by size: 2042 1434 966 648 359 282 159 MspA1I CmG'CkG Cuts at: 1606 2119 2975 3306 3983 5131 5376 1606 Size: 513 856 331 677 1148 245 2120 Fragments arranged by size: 2120 1148 856 677 513 331 245 NaeI GCC'GGC Cuts at: 2584 2584 Size: 5890 NarI GG'CG_CC Cuts at: 2122 2122 Size: 5890 NcoI C'CATG_G Cuts at: 513 1065 2004 3075 513 Size: 552 939 1071 3328 Fragments arranged by size: 3328 1071 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5890 NgoMIV G'CCGG_C Cuts at: 2582 2582 Size: 5890 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5890 NotI GC'GGCC_GC Cuts at: 3337 3337 Size: 5890 NspI r_CATG'y Cuts at: 1132 1229 3248 4793 1132 Size: 97 2019 1545 2229 Fragments arranged by size: 2229 2019 1545 97 PasI CC'CwG_GG Cuts at: 1348 2857 1348 Size: 1509 4381 PciI A'CATG_T Cuts at: 4789 4789 Size: 5890 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5890 PfoI T'CCnGG_A Cuts at: 2727 2838 2727 Size: 111 5779 PmeI GTTT'AAAC Cuts at: 3194 3194 Size: 5890 PmlI CAC'GTG Cuts at: 2149 2149 Size: 5890 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3959 3991 5472 5504 3959 Size: 32 1481 32 4345 Fragments arranged by size: 4345 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2376 2715 2752 2376 Size: 339 37 5514 Fragments arranged by size: 5514 339 37 PshAI GACnn'nnGTC Cuts at: 1613 2381 1613 Size: 768 5122 PsiI TTA'TAA Cuts at: 3460 3460 Size: 5890 PspXI vC'TCGA_Gb Cuts at: 2075 2201 2075 Size: 126 5764 PstI C_TGCA'G Cuts at: 830 2219 3242 830 Size: 1389 1023 3478 Fragments arranged by size: 3478 1389 1023 PvuI CG_AT'CG Cuts at: 2001 2590 2001 Size: 589 5301 SacI G_AGCT'C Cuts at: 2049 2280 2049 Size: 231 5659 SalI G'TCGA_C Cuts at: 1936 2735 3232 1936 Size: 799 497 4594 Fragments arranged by size: 4594 799 497 SanDI GG'GwC_CC Cuts at: 2376 2715 2376 Size: 339 5551 SbfI CC_TGCA'GG Cuts at: 3242 3242 Size: 5890 ScaI AGT'ACT Cuts at: 4138 5850 4138 Size: 1712 4178 SfcI C'TryA_G Cuts at: 826 1045 2215 3238 4373 5054 5245 826 Size: 219 1170 1023 1135 681 191 1471 Fragments arranged by size: 1471 1170 1135 1023 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2168 2168 Size: 5890 SfoI GGC'GCC Cuts at: 2123 2123 Size: 5890 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 SgrAI Cr'CCGG_yG Cuts at: 1700 2582 1700 Size: 882 5008 SmaI CCC'GGG Cuts at: 1872 3217 1872 Size: 1345 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2075 2201 2899 3994 4895 5157 Size: 197 926 132 126 698 1095 901 262 Cuts at: 5157 820 Size: 1553 Fragments arranged by size: 1553 1095 926 901 698 262 197 132 126 SnaBI TAC'GTA Cuts at: 493 3681 493 Size: 3188 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5890 SphI G_CATG'C Cuts at: 1132 3248 1132 Size: 2116 3774 SspI AAT'ATT Cuts at: 5 52 3814 5 Size: 47 3762 2081 Fragments arranged by size: 3762 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5890 StyI C'CwwG_G Cuts at: 513 1065 2004 2657 3075 3120 3329 513 Size: 552 939 653 418 45 209 3074 Fragments arranged by size: 3074 939 653 552 418 209 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3917 3934 4087 4272 4717 325 Size: 1255 2337 17 153 185 445 1498 Fragments arranged by size: 2337 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4136 5848 96 Size: 274 80 33 51 1443 2159 1712 138 Fragments arranged by size: 2159 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5850 5882 5850 Size: 32 5858 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5890 XbaI T'CTAG_A Cuts at: 3226 3226 Size: 5890 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2836 2836 Size: 5890 XhoI C'TCGA_G Cuts at: 1943 2075 2201 1943 Size: 132 126 5632 Fragments arranged by size: 5632 132 126 XmaI C'CCGG_G Cuts at: 1870 3215 1870 Size: 1345 4545 XmnI GAAnn'nnTTC Cuts at: 4019 4019 Size: 5890 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2734 3698 276 Size: 53 83 186 936 1200 964 2468 Fragments arranged by size: 2468 1200 964 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PspOMI PsrI PvuII RsrII SacII SapI SexAI SrfI SwaI