################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AccIII AclI AflII AflIII AgeI AhdI AlwI ApaI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbsI BbvCI BcgI BfaI BfrI BglII BlnI BpuAI BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HpaI KspI MaeI MamI MfeI MflI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmeI PshBI Psp1406I PvuII RcaI RsrII SacII SapI SbfI ScaI SexAI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I Van91I XbaI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 727 GACGTC GACGT^C Acc65I 524 GGTACC G^GTACC AccI 730 GTCGAC GT^[AC][GT]AC AcsI 1122 GAATTC [GA]^AATT[CT] Alw44I 430 GTGCAC G^TGCAC AlwNI 240 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1045 CCTGAGG CC^T[ACGT]AGG ApaLI 430 GTGCAC G^TGCAC ApoI 1122 GAATTC [GA]^AATT[CT] Asp718I 524 GGTACC G^GTACC BbeI 116 GGCGCC GGCGC^C BbrPI 142 CACGTG CAC^GTG BciVI 921 GGATAC GTATCC(6/5) BclI 981 TGATCA T^GATCA BpmI 835 CTGGAG CTGGAG(16/14) BsaAI 142 CACGTG [CT]AC^GT[GA] BsaI 664 GAGACC GGTCTC(1/5) BsaWI 388 ACCGGA [AT]^CCGG[AT] BseRI 1048 GAGGAG GAGGAG(10/8) BsmAI 664 GAGAC GTCTC(1/5) BspMI 343 ACCTGC ACCTGC(4/8) BsrBI 21 GAGCGG CCGCTC(-3/-3) BsrDI 825 CATTGC GCAATG(2/0) BssHII 479 GCGCGC G^CGCGC BstAPI 77 GCAAGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 386 CCACCGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1045 CCTGAGG CC^T[ACGT]AGG DsaI 1070 CCATGG C^C[GA][CT]GG EarI 1127 CTCTTC CTCTTC(1/4) Eco81I 1045 CCTGAGG CC^T[ACGT]AGG EcoRI 1122 GAATTC G^AATTC FbaI 981 TGATCA T^GATCA HindIII 622 AAGCTT A^AGCTT HinfI 463 GACTC G^A[ACGT]TC KasI 116 GGCGCC G^GCGCC KpnI 524 GGTACC GGTAC^C Ksp632I 1127 CTCTTC CTCTTC(1/4) MboII 1128 TCTTC GAAGA(8/7) NaeI 577 GCCGGC GCC^GGC NarI 116 GGCGCC GG^CGCC NcoI 1070 CCATGG C^CATGG NgoMIV 577 GCCGGC G^CCGGC PleI 463 GACTC GAGTC(4/5) PmaCI 142 CACGTG CAC^GTG PmlI 142 CACGTG CAC^GTG PshAI 372 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 210 CTGCAG CTGCA^G PvuI 582 CGATCG CGAT^CG SalI 730 GTCGAC G^TCGAC SfcI 210 CTGCAG C^T[GA][CT]AG SfiI 156 GGCCTGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 116 GGCGCC GGC^GCC SgrAI 576 CGCCGGCG C[GA]^CCGG[CT]G XcmI 824 CCATTGCGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 525 GTAC GT^AC 1060 GTAC Aor51HI 30 AGCGCT AGC^GCT 285 AGCGCT BalI 99 TGGCCA TGG^CCA 612 TGGCCA BanI 116 GGCGCC G^G[CT][GA]CC 524 GGTACC BglI 157 GCCTGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 166 GCCGCCCTGGC BlpI 281 GCTCAGC GC^T[ACGT]AGC 933 GCTGAGC Bpu1102I 281 GCTCAGC GC^T[ACGT]AGC 933 GCTGAGC BsgI 259 CTGCAC GTGCAG(16/14) 715 CTGCAC CelII 281 GCTCAGC GC^T[ACGT]AGC 933 GCTGAGC EagI 5 CGGCCG C^GGCCG 302 CGGCCG EclXI 5 CGGCCG C^GGCCG 302 CGGCCG Eco47III 30 AGCGCT AGC^GCT 285 AGCGCT Eco52I 5 CGGCCG C^GGCCG 302 CGGCCG HgaI 505 GACGC GACGC(5/10) 574 GACGC MaeII 143 ACGT A^CGT 728 ACGT MluNI 99 TGGCCA TGG^CCA 612 TGGCCA MscI 99 TGGCCA TGG^CCA 612 TGGCCA MslI 591 CATCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 873 CACCAGGGTG MspA1I 112 CAGCGG C[AC]G^C[GT]G 968 CCGCTG PaeR7I 70 CTCGAG C^TCGAG 196 CTCGAG RsaI 525 GTAC GT^AC 1060 GTAC SacI 40 GAGCTC GAGCT^C 271 GAGCTC Tsp509I 641 AATT ^AATT 1123 AATT TspRI 570 CACTG CA[GC]TG[ACGT][ACGT]^ 803 CAGTG XhoI 70 CTCGAG C^TCGAG 196 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AcyI AluI AspHI AvaI AvaII BanII BbvI BcnI BmyI BsaHI BsaJI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HpaII HphI ItaI MaeIII MboI MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TseI Tsp45I TthHB8I