GPR27 ORF clone FHC21601

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC21601

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Clone info.

Species

human

Clone Name

pFN21AE0811 Sequence (with vector) Restriction Map

Product ID

FHC21601

ORF Sequence

DNA sequence (1125 bp)
Protein sequence (375 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE0811

Symbol

GPR27

Alias : SREB1

Description

G protein-coupled receptor 27

Original Clone ID

cp01211

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1125 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 375 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2915.1 2.3e-111 100.0 GPR27 CCDS14349.1 1.3e-57 54.7 GPR173 CCDS5758.1 5.2e-55 55.2 GPR85 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 20 44 PR00237 GPCR IPR000276 53 74 PR00237 GPCR IPR000276 102 124 PR00237 GPCR IPR000276 139 160 PR00237 GPCR IPR000276 282 306 PR00237 GPCR IPR000276 320 346 PR00237 GPCR HMMPfam IPR000276 35 337 PF00001 GPCR ProfileScan IPR017452 35 338 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 19 GLKLATLSLLLCVSLAGNVLFAL 41 PRIMARY 23 2 55 YLLLDLCLADGLRALACLPAVML 77 SECONDARY 23 3 100 FLAALFCFHAAFLLLGVGVTRY 121 PRIMARY 22 4 138 PCAAMLVCAAWALALAAAFPPVL 160 PRIMARY 23 5 184 LGFLLLLAVVVGATHLVYLRLLF 206 PRIMARY 23 6 286 FYAVTLLFLLLWGPYVVASYLRV 308 PRIMARY 23 7 319 YLTASVWLTFAQAGINPVVCFLF 341 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_061844 375 605187 100.0 100.0 NP_061842 373 300253 54.7 94.7 XP_011529100 373 300253 54.7 94.7 NP_001139739 370 605188 55.2 90.9 NP_061843 370 605188 55.2 90.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0811 Download

>KIEE0811  1125 bp
ATGGCGAACGCGAGCGAGCCGGGTGGCAGCGGCGGCGGCGAGGCGGCCGCCCTGGGCCTC
AAGCTGGCCACGCTCAGCCTGCTGCTGTGCGTGAGCCTAGCGGGCAACGTGCTGTTCGCG
CTGCTGATCGTGCGGGAGCGCAGCCTGCACCGCGCCCCGTACTACCTGCTGCTCGACCTG
TGCCTGGCCGACGGGCTGCGCGCGCTCGCCTGCCTCCCGGCCGTCATGCTGGCGGCGCGG
CGTGCGGCGGCCGCGGCGGGGGCGCCGCCGGGCGCGCTGGGCTGCAAGCTGCTCGCCTTC
CTGGCCGCGCTCTTCTGCTTCCACGCCGCCTTCCTGCTGCTGGGCGTGGGCGTCACCCGC
TACCTGGCCATCGCGCACCACCGCTTCTATGCAGAGCGCCTGGCCGGCTGGCCGTGCGCC
GCCATGCTGGTGTGCGCCGCCTGGGCGCTGGCGCTGGCCGCGGCCTTCCCGCCAGTGCTG
GACGGCGGTGGCGACGACGAGGACGCGCCGTGCGCCCTGGAGCAGCGGCCCGACGGCGCC
CCCGGCGCGCTGGGCTTCCTGCTGCTGCTGGCCGTGGTGGTGGGCGCCACGCACCTCGTC
TACCTCCGCCTGCTCTTCTTCATCCACGACCGCCGCAAGATGCGGCCCGCGCGCCTGGTG
CCCGCCGTCAGCCACGACTGGACCTTCCACGGCCCGGGCGCCACCGGCCAGGCGGCCGCC
AACTGGACGGCGGGCTTCGGCCGCGGGCCCACGCCGCCCGCGCTTGTGGGCATCCGGCCC
GCAGGGCCGGGCCGCGGCGCGCGCCGCCTCCTCGTGCTGGAAGAATTCAAGACGGAGAAG
AGGCTGTGCAAGATGTTCTACGCCGTCACGCTGCTCTTCCTGCTCCTCTGGGGGCCCTAC
GTCGTGGCCAGCTACCTGCGGGTCCTGGTGCGGCCCGGCGCCGTCCCCCAGGCCTACCTG
ACGGCCTCCGTGTGGCTGACCTTCGCGCAGGCCGGCATCAACCCCGTCGTGTGCTTCCTC
TTCAACAGGGAGCTGAGGGACTGCTTCAGGGCCCAGTTCCCCTGCTGCCAGAGCCCCCGG
ACCACCCAGGCGACCCATCCCTGCGACCTGAAAGGCATTGGTTTA

ORF Protein

Cloned ORF protein sequence for pFN21AE0811 Download

>KIEE0811  375 aa
MANASEPGGSGGGEAAALGLKLATLSLLLCVSLAGNVLFALLIVRERSLHRAPYYLLLDL
CLADGLRALACLPAVMLAARRAAAAAGAPPGALGCKLLAFLAALFCFHAAFLLLGVGVTR
YLAIAHHRFYAERLAGWPCAAMLVCAAWALALAAAFPPVLDGGGDDEDAPCALEQRPDGA
PGALGFLLLLAVVVGATHLVYLRLLFFIHDRRKMRPARLVPAVSHDWTFHGPGATGQAAA
NWTAGFGRGPTPPALVGIRPAGPGRGARRLLVLEEFKTEKRLCKMFYAVTLLFLLLWGPY
VVASYLRVLVRPGAVPQAYLTASVWLTFAQAGINPVVCFLFNRELRDCFRAQFPCCQSPR
TTQATHPCDLKGIGL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0811 Download

>pFN21AE0811  5830 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGAACGCGAGCGAGCCGGGTGGCAGCGGCGG
CGGCGAGGCGGCCGCCCTGGGCCTCAAGCTGGCCACGCTCAGCCTGCTGCTGTGCGTGAG
CCTAGCGGGCAACGTGCTGTTCGCGCTGCTGATCGTGCGGGAGCGCAGCCTGCACCGCGC
CCCGTACTACCTGCTGCTCGACCTGTGCCTGGCCGACGGGCTGCGCGCGCTCGCCTGCCT
CCCGGCCGTCATGCTGGCGGCGCGGCGTGCGGCGGCCGCGGCGGGGGCGCCGCCGGGCGC
GCTGGGCTGCAAGCTGCTCGCCTTCCTGGCCGCGCTCTTCTGCTTCCACGCCGCCTTCCT
GCTGCTGGGCGTGGGCGTCACCCGCTACCTGGCCATCGCGCACCACCGCTTCTATGCAGA
GCGCCTGGCCGGCTGGCCGTGCGCCGCCATGCTGGTGTGCGCCGCCTGGGCGCTGGCGCT
GGCCGCGGCCTTCCCGCCAGTGCTGGACGGCGGTGGCGACGACGAGGACGCGCCGTGCGC
CCTGGAGCAGCGGCCCGACGGCGCCCCCGGCGCGCTGGGCTTCCTGCTGCTGCTGGCCGT
GGTGGTGGGCGCCACGCACCTCGTCTACCTCCGCCTGCTCTTCTTCATCCACGACCGCCG
CAAGATGCGGCCCGCGCGCCTGGTGCCCGCCGTCAGCCACGACTGGACCTTCCACGGCCC
GGGCGCCACCGGCCAGGCGGCCGCCAACTGGACGGCGGGCTTCGGCCGCGGGCCCACGCC
GCCCGCGCTTGTGGGCATCCGGCCCGCAGGGCCGGGCCGCGGCGCGCGCCGCCTCCTCGT
GCTGGAAGAATTCAAGACGGAGAAGAGGCTGTGCAAGATGTTCTACGCCGTCACGCTGCT
CTTCCTGCTCCTCTGGGGGCCCTACGTCGTGGCCAGCTACCTGCGGGTCCTGGTGCGGCC
CGGCGCCGTCCCCCAGGCCTACCTGACGGCCTCCGTGTGGCTGACCTTCGCGCAGGCCGG
CATCAACCCCGTCGTGTGCTTCCTCTTCAACAGGGAGCTGAGGGACTGCTTCAGGGCCCA
GTTCCCCTGCTGCCAGAGCCCCCGGACCACCCAGGCGACCCATCCCTGCGACCTGAAAGG
CATTGGTTTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT