(Circular) (Six-base) MAPSORT of: pFN21AE0811.seq Check: 401 from: 1 to: 5830 pFN21AE0811 5830 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3640 278 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2604 3173 1937 Size: 667 569 4594 Fragments arranged by size: 4594 667 569 Acc65I G'GTAC_C Cuts at: 3151 3151 Size: 5830 AclI AA'CG_TT Cuts at: 1563 3957 4330 1563 Size: 2394 373 3063 Fragments arranged by size: 3063 2394 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3034 3893 5277 5545 1632 Size: 1402 859 1384 268 1917 Fragments arranged by size: 1917 1402 1384 859 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5633 AflIII A'CryG_T Cuts at: 1203 4729 5706 1203 Size: 3526 977 1327 Fragments arranged by size: 3526 1327 977 AgeI A'CCGG_T Cuts at: 4661 4661 Size: 5830 AhdI GACnn_n'nnGTC Cuts at: 4559 4559 Size: 5830 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5830 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5830 AlwNI CAG_nnn'CTG Cuts at: 3573 5145 3573 Size: 1572 4258 ApaI G_GGCC'C Cuts at: 2754 2901 3058 2754 Size: 147 157 5526 Fragments arranged by size: 5526 157 147 ApaLI G'TGCA_C Cuts at: 3887 5043 3887 Size: 1156 4674 ApoI r'AATT_y Cuts at: 1493 2828 3139 3365 3517 5626 1493 Size: 1335 311 226 152 2109 1697 Fragments arranged by size: 2109 1697 1335 311 226 152 AseI AT'TA_AT Cuts at: 160 1032 4384 160 Size: 872 3352 1606 Fragments arranged by size: 3352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 AvaI C'yCGr_G Cuts at: 1870 1943 2698 3155 1870 Size: 73 755 457 4545 Fragments arranged by size: 4545 755 457 73 BamHI G'GATC_C Cuts at: 3160 3527 3160 Size: 367 5463 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2266 2540 2588 2662 2702 Size: 325 217 627 479 274 48 74 40 Cuts at: 2702 2942 3151 4606 618 Size: 240 209 1455 1842 Fragments arranged by size: 1842 1455 627 479 325 274 240 217 209 74 48 40 BanII G_rGCy'C Cuts at: 1391 2754 2901 3058 3080 3149 1391 Size: 1363 147 157 22 69 4072 Fragments arranged by size: 4072 1363 157 147 69 22 BbeI G_GCGC'C Cuts at: 2270 2544 2592 2706 2946 2270 Size: 274 48 114 240 5154 Fragments arranged by size: 5154 274 240 114 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4859 BbvCI CC'TCA_GC Cuts at: 3038 3038 Size: 5830 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2278 2312 4021 4055 1903 Size: 34 341 34 1709 34 3678 Fragments arranged by size: 3678 1709 341 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3722 4932 3722 Size: 1210 4620 BclI T'GATC_A Cuts at: 1548 1548 Size: 5830 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2106 2306 2409 Size: 107 122 71 631 605 434 200 103 Cuts at: 2409 2447 2788 4441 136 Size: 38 341 1653 1525 Fragments arranged by size: 1653 1525 631 605 434 341 200 122 107 103 71 38 BglII A'GATC_T Cuts at: 5825 5825 Size: 5830 BlpI GC'TnA_GC Cuts at: 2078 3247 2078 Size: 1169 4661 Bme1580I G_kGCm'C Cuts at: 1790 2667 2754 2901 3058 3891 5047 1790 Size: 877 87 147 157 833 1156 2573 Fragments arranged by size: 2573 1156 877 833 157 147 87 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5830 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3052 3561 4519 Size: 416 53 83 399 24 1635 509 958 Cuts at: 4519 442 Size: 1753 Fragments arranged by size: 1753 1635 958 509 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2543 4490 1239 Size: 1304 1947 2579 Fragments arranged by size: 2579 1947 1304 Bpu10I CC'TnA_GC Cuts at: 3038 3038 Size: 5830 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2048 3955 4820 5118 2048 Size: 1907 865 298 2760 Fragments arranged by size: 2760 1907 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4493 882 Size: 617 2994 2219 Fragments arranged by size: 2994 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3621 493 Size: 711 2417 2702 Fragments arranged by size: 2702 2417 711 BsaBI GATnn'nnATC Cuts at: 5824 5824 Size: 5830 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2267 2355 2541 2589 Size: 53 83 186 936 734 88 186 48 Cuts at: 2589 2703 2943 3637 4019 4654 275 Size: 114 240 694 382 635 1451 Fragments arranged by size: 1451 936 734 694 635 382 240 186 186 114 88 83 53 48 BsaWI w'CCGG_w Cuts at: 4263 4661 4935 5082 5493 4263 Size: 398 274 147 411 4600 Fragments arranged by size: 4600 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5605 Fragments arranged by size: 5605 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2804 2879 1186 Size: 1618 75 4137 Fragments arranged by size: 4137 1618 75 BseYI C'CCAG_C Cuts at: 2281 2344 2554 5033 5529 2281 Size: 63 210 2479 496 2582 Fragments arranged by size: 2582 2479 496 210 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2135 1163 Size: 573 399 4858 Fragments arranged by size: 4858 573 399 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2052 2226 2256 2635 2721 2746 Size: 275 186 51 174 30 379 86 25 Cuts at: 2746 3280 4041 5069 1540 Size: 534 761 1028 2301 Fragments arranged by size: 2301 1028 761 534 379 275 186 174 86 51 30 25 BsiHKAI G_wGCw'C Cuts at: 3891 3976 5047 3891 Size: 85 1071 4674 Fragments arranged by size: 4674 1071 85 BsmI GAATG_Cn' Cuts at: 3341 3434 3341 Size: 93 5737 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2667 2754 2901 3058 3080 3149 3891 Size: 399 877 87 147 157 22 69 742 Cuts at: 3891 3976 5047 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 877 742 399 157 147 87 85 69 22 BspEI T'CCGG_A Cuts at: 5493 5493 Size: 5830 BspHI T'CATG_A Cuts at: 3719 5449 3719 Size: 1730 4100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2178 2928 3185 844 Size: 1334 750 257 3489 Fragments arranged by size: 3489 1334 750 257 BsrBI CCG'CTC Cuts at: 1394 1823 3717 1394 Size: 429 1894 3507 Fragments arranged by size: 3507 1894 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4325 4499 65 Size: 1183 3077 174 1396 Fragments arranged by size: 3077 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2408 2708 2996 4474 4661 1700 Size: 110 598 300 288 1478 187 2869 Fragments arranged by size: 2869 1478 598 300 288 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5830 BssHII G'CGCG_C Cuts at: 1925 2203 2205 2277 2550 2654 2802 2804 1925 Size: 278 2 72 273 104 148 2 4951 Fragments arranged by size: 4951 278 273 148 104 72 2 2 BssSI C'ACGA_G Cuts at: 2816 3890 4902 2816 Size: 1074 1012 3744 Fragments arranged by size: 3744 1074 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5830 BstBI TT'CG_AA Cuts at: 4634 4634 Size: 5830 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5830 BstYI r'GATC_y Cuts at: 1972 3160 3527 3912 3929 5381 5467 5479 5825 Size: 1188 367 385 17 1452 86 12 346 Cuts at: 5825 1972 Size: 1977 Fragments arranged by size: 1977 1452 1188 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2256 2463 2577 2692 2746 2797 Size: 552 939 252 207 114 115 54 51 Cuts at: 2797 513 Size: 3546 Fragments arranged by size: 3546 939 552 252 207 115 114 54 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2359 487 Size: 683 56 1133 3958 Fragments arranged by size: 3958 1133 683 56 BtsI GCAGTG_nn' Cuts at: 3346 4140 4160 3346 Size: 794 20 5016 Fragments arranged by size: 5016 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5795 DraI TTT'AAA Cuts at: 3134 3134 Size: 5830 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4837 809 Size: 507 3521 1802 Fragments arranged by size: 3521 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2070 2190 Size: 54 1425 50 275 18 219 21 120 Cuts at: 2190 2223 2253 2307 2370 2406 2414 2460 2574 Size: 33 30 54 63 36 8 46 114 Cuts at: 2574 2710 2718 2743 2910 3277 4166 5795 8 Size: 136 8 25 167 367 889 1629 43 Fragments arranged by size: 1629 1425 889 367 275 219 167 136 120 114 63 54 54 50 46 43 36 33 30 25 21 18 8 8 EagI C'GGCC_G Cuts at: 1537 1812 2049 2223 2253 2718 2743 3277 1537 Size: 275 237 174 30 465 25 534 4090 Fragments arranged by size: 4090 534 465 275 237 174 30 25 EarI CTCTTCn'nnn_ Cuts at: 1353 2321 2624 2837 2885 3029 3760 1353 Size: 968 303 213 48 144 731 3423 Fragments arranged by size: 3423 968 731 303 213 144 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2600 4417 4791 4937 387 Size: 1064 138 1011 1817 374 146 1280 Fragments arranged by size: 1817 1280 1064 1011 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2543 3034 3893 4490 5277 5545 1239 Size: 393 911 491 859 597 787 268 1524 Fragments arranged by size: 1524 911 859 787 597 491 393 268 EcoO109I rG'GnC_Cy Cuts at: 2897 2898 2926 3054 2897 Size: 1 28 128 5673 Fragments arranged by size: 5673 128 28 1 EcoRI G'AATT_C Cuts at: 2828 3139 3517 2828 Size: 311 378 5141 Fragments arranged by size: 5141 378 311 FspI TGC'GCA Cuts at: 4336 4336 Size: 5830 HaeII r_GCGC'y Cuts at: 1678 2270 2404 2453 2459 2544 2592 2706 2946 Size: 592 134 49 6 85 48 114 240 Cuts at: 2946 4977 1678 Size: 2031 2531 Fragments arranged by size: 2531 2031 592 240 134 114 85 49 48 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4471 4503 4545 4577 1373 Size: 32 135 32 2899 32 42 32 2626 Fragments arranged by size: 2899 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3174 3420 669 Size: 988 281 1236 246 3079 Fragments arranged by size: 3079 1236 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5830 HpaI GTT'AAC Cuts at: 3420 3420 Size: 5830 KasI G'GCGC_C Cuts at: 2266 2540 2588 2702 2942 2266 Size: 274 48 114 240 5154 Fragments arranged by size: 5154 274 240 114 48 KpnI G_GTAC'C Cuts at: 3155 3155 Size: 5830 MfeI C'AATT_G Cuts at: 3429 3429 Size: 5830 MluI A'CGCG_T Cuts at: 5706 5706 Size: 5830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4944 5128 1305 Size: 3639 184 2007 Fragments arranged by size: 3639 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2072 2372 2912 10 Size: 54 1425 343 240 300 540 2928 Fragments arranged by size: 2928 1425 540 343 300 240 54 MslI CAynn'nnrTG Cuts at: 518 1166 2432 3788 4147 4306 518 Size: 648 1266 1356 359 159 2042 Fragments arranged by size: 2042 1356 1266 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2034 2258 2465 2530 2748 2799 3246 3923 Size: 428 224 207 65 218 51 447 677 Cuts at: 3923 5071 5316 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 447 428 245 224 218 207 65 51 NaeI GCC'GGC Cuts at: 2410 2998 2410 Size: 588 5242 NarI GG'CG_CC Cuts at: 2267 2541 2589 2703 2943 2267 Size: 274 48 114 240 5154 Fragments arranged by size: 5154 274 240 114 48 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4339 Fragments arranged by size: 4339 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5830 NgoMIV G'CCGG_C Cuts at: 2408 2996 2408 Size: 588 5242 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5830 NotI GC'GGCC_GC Cuts at: 2049 2253 2718 3277 2049 Size: 204 465 559 4602 Fragments arranged by size: 4602 559 465 204 NspI r_CATG'y Cuts at: 1132 1229 3188 4733 1132 Size: 97 1959 1545 2229 Fragments arranged by size: 2229 1959 1545 97 PasI CC'CwG_GG Cuts at: 1348 2056 1348 Size: 708 5122 PciI A'CATG_T Cuts at: 4729 4729 Size: 5830 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5830 PmeI GTTT'AAAC Cuts at: 3134 3134 Size: 5830 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3899 3931 5412 5444 3899 Size: 32 1481 32 4285 Fragments arranged by size: 4285 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2926 2926 Size: 5830 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5830 PsiI TTA'TAA Cuts at: 3400 3400 Size: 5830 PspOMI G'GGCC_C Cuts at: 2750 2897 3054 2750 Size: 147 157 5526 Fragments arranged by size: 5526 157 147 PstI C_TGCA'G Cuts at: 830 3182 830 Size: 2352 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5830 SacII CC_GC'GG Cuts at: 2259 2466 2749 2800 2259 Size: 207 283 51 5289 Fragments arranged by size: 5289 283 207 51 SalI G'TCGA_C Cuts at: 1936 3172 1936 Size: 1236 4594 SapI GCTCTTCn'nnn_ Cuts at: 2321 2624 2885 2321 Size: 303 261 5266 Fragments arranged by size: 5266 303 261 SbfI CC_TGCA'GG Cuts at: 3182 3182 Size: 5830 ScaI AGT'ACT Cuts at: 4078 5790 4078 Size: 1712 4118 SfcI C'TryA_G Cuts at: 826 1045 3178 4313 4994 5185 826 Size: 219 2133 1135 681 191 1471 Fragments arranged by size: 2133 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2788 2788 Size: 5830 SfoI GGC'GCC Cuts at: 2268 2542 2590 2704 2944 2268 Size: 274 48 114 240 5154 Fragments arranged by size: 5154 274 240 114 48 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5830 SmaI CCC'GGG Cuts at: 1872 2700 3157 1872 Size: 828 457 4545 Fragments arranged by size: 4545 828 457 SmlI C'TyrA_G Cuts at: 820 1017 1943 2063 3934 4835 5097 820 Size: 197 926 120 1871 901 262 1553 Fragments arranged by size: 1871 1553 926 901 262 197 120 SnaBI TAC'GTA Cuts at: 493 3621 493 Size: 3128 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5830 SphI G_CATG'C Cuts at: 1132 3188 1132 Size: 2056 3774 SrfI GCCC'GGGC Cuts at: 2700 2700 Size: 5830 SspI AAT'ATT Cuts at: 5 52 3754 5 Size: 47 3702 2081 Fragments arranged by size: 3702 2081 47 StuI AGG'CCT Cuts at: 1517 2957 1517 Size: 1440 4390 StyI C'CwwG_G Cuts at: 513 1065 2004 3269 513 Size: 552 939 1265 3074 Fragments arranged by size: 3074 1265 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3104 3857 3874 4027 4212 4657 325 Size: 1255 1524 753 17 153 185 445 1498 Fragments arranged by size: 1524 1498 1255 753 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4076 5788 96 Size: 274 80 33 51 1443 2099 1712 138 Fragments arranged by size: 2099 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2498 2530 5790 5822 2498 Size: 32 3260 32 2506 Fragments arranged by size: 3260 2506 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5830 XbaI T'CTAG_A Cuts at: 3166 3166 Size: 5830 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5830 XmaI C'CCGG_G Cuts at: 1870 2698 3155 1870 Size: 828 457 4545 Fragments arranged by size: 4545 828 457 XmnI GAAnn'nnTTC Cuts at: 3959 3959 Size: 5830 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3638 276 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SanDI SexAI SwaI XcmI