Restriction map for
pFN21AE0811
(Linear) (Six-base) MAPSORT of: KIEE0811.seq Check: 2440 from: 1 to: 1125
KIEE0811.orf
With 182 enzymes: SgfI *
December 27, 2011 16:31 ..
AccI GT'mk_AC
Cuts at: 0 599 1125
Size: 599 526
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1029 1125
Size: 1029 96
ApaI G_GGCC'C
Cuts at: 0 749 896 1053 1125
Size: 749 147 157 72
Fragments arranged by size:
749 157 147 72
ApoI r'AATT_y
Cuts at: 0 823 1125
Size: 823 302
AvaI C'yCGr_G
Cuts at: 0 693 1125
Size: 693 432
BanI G'GyrC_C
Cuts at: 0 261 535 583 657 697 937 1125
Size: 261 274 48 74 40 240 188
Fragments arranged by size:
274 261 240 188 74 48 40
BanII G_rGCy'C
Cuts at: 0 749 896 1053 1075 1125
Size: 749 147 157 22 50
Fragments arranged by size:
749 157 147 50 22
BbeI G_GCGC'C
Cuts at: 0 265 539 587 701 941 1125
Size: 265 274 48 114 240 184
Fragments arranged by size:
274 265 240 184 114 48
BbvCI CC'TCA_GC
Cuts at: 0 1033 1125
Size: 1033 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 273 307 1125
Size: 273 34 818
Fragments arranged by size:
818 273 34
BglI GCCn_nnn'nGGC
Cuts at: 0 101 301 404 442 783 1125
Size: 101 200 103 38 341 342
Fragments arranged by size:
342 341 200 103 101 38
BlpI GC'TnA_GC
Cuts at: 0 73 1125
Size: 73 1052
Bme1580I G_kGCm'C
Cuts at: 0 662 749 896 1053 1125
Size: 662 87 147 157 72
Fragments arranged by size:
662 157 147 87 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 1047 1125
Size: 1047 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1125
Size: 538 587
Bpu10I CC'TnA_GC
Cuts at: 0 1033 1125
Size: 1033 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 1125
Size: 43 1082
BsaHI Gr'CG_yC
Cuts at: 0 262 350 536 584 698 938 1125
Size: 262 88 186 48 114 240 187
Fragments arranged by size:
262 240 187 186 114 88 48
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 799 874 1125
Size: 799 75 251
Fragments arranged by size:
799 251 75
BseYI C'CCAG_C
Cuts at: 0 276 339 549 1125
Size: 276 63 210 576
Fragments arranged by size:
576 276 210 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 1125
Size: 130 995
BsiEI CG_ry'CG
Cuts at: 0 47 221 251 630 716 741 1125
Size: 47 174 30 379 86 25 384
Fragments arranged by size:
384 379 174 86 47 30 25
Bsp1286I G_dGCh'C
Cuts at: 0 662 749 896 1053 1075 1125
Size: 662 87 147 157 22 50
Fragments arranged by size:
662 157 147 87 50 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 173 923 1125
Size: 173 750 202
Fragments arranged by size:
750 202 173
BsrFI r'CCGG_y
Cuts at: 0 403 703 991 1125
Size: 403 300 288 134
Fragments arranged by size:
403 300 288 134
BssHII G'CGCG_C
Cuts at: 0 198 200 272 545 649 797 799 1125
Size: 198 2 72 273 104 148 2 326
Fragments arranged by size:
326 273 198 148 104 72 2 2
BssSI C'ACGA_G
Cuts at: 0 811 1125
Size: 811 314
BtgI C'CryG_G
Cuts at: 0 251 458 572 687 741 792 1125
Size: 251 207 114 115 54 51 333
Fragments arranged by size:
333 251 207 115 114 54 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 354 1125
Size: 354 771
EaeI y'GGCC_r
Cuts at: 0 44 65 185 218 248 302 365 401
Size: 44 21 120 33 30 54 63 36
Cuts at: 401 409 455 569 705 713 738 905 1125
Size: 8 46 114 136 8 25 167 220
Fragments arranged by size:
220 167 136 120 114 63 54 46
44 36 33 30 25 21 8 8
EagI C'GGCC_G
Cuts at: 0 44 218 248 713 738 1125
Size: 44 174 30 465 25 387
Fragments arranged by size:
465 387 174 44 30 25
EarI CTCTTCn'nnn_
Cuts at: 0 316 619 832 880 1024 1125
Size: 316 303 213 48 144 101
Fragments arranged by size:
316 303 213 144 101 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 595 1125
Size: 595 530
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1029 1125
Size: 538 491 96
Fragments arranged by size:
538 491 96
EcoO109I rG'GnC_Cy
Cuts at: 0 892 893 921 1049 1125
Size: 892 1 28 128 76
Fragments arranged by size:
892 128 76 28 1
EcoRI G'AATT_C
Cuts at: 0 823 1125
Size: 823 302
HaeII r_GCGC'y
Cuts at: 0 265 399 448 454 539 587 701 941
Size: 265 134 49 6 85 48 114 240
Cuts at: 941 1125
Size: 184
Fragments arranged by size:
265 240 184 134 114 85 49 48
6
KasI G'GCGC_C
Cuts at: 0 261 535 583 697 937 1125
Size: 261 274 48 114 240 188
Fragments arranged by size:
274 261 240 188 114 48
MscI TGG'CCA
Cuts at: 0 67 367 907 1125
Size: 67 300 540 218
Fragments arranged by size:
540 300 218 67
MslI CAynn'nnrTG
Cuts at: 0 427 1125
Size: 427 698
MspA1I CmG'CkG
Cuts at: 0 29 253 460 525 743 794 1125
Size: 29 224 207 65 218 51 331
Fragments arranged by size:
331 224 218 207 65 51 29
NaeI GCC'GGC
Cuts at: 0 405 993 1125
Size: 405 588 132
Fragments arranged by size:
588 405 132
NarI GG'CG_CC
Cuts at: 0 262 536 584 698 938 1125
Size: 262 274 48 114 240 187
Fragments arranged by size:
274 262 240 187 114 48
NgoMIV G'CCGG_C
Cuts at: 0 403 991 1125
Size: 403 588 134
Fragments arranged by size:
588 403 134
NotI GC'GGCC_GC
Cuts at: 0 44 248 713 1125
Size: 44 204 465 412
Fragments arranged by size:
465 412 204 44
PasI CC'CwG_GG
Cuts at: 0 51 1125
Size: 51 1074
PpuMI rG'GwC_Cy
Cuts at: 0 921 1125
Size: 921 204
PspOMI G'GGCC_C
Cuts at: 0 745 892 1049 1125
Size: 745 147 157 76
Fragments arranged by size:
745 157 147 76
SacII CC_GC'GG
Cuts at: 0 254 461 744 795 1125
Size: 254 207 283 51 330
Fragments arranged by size:
330 283 254 207 51
SapI GCTCTTCn'nnn_
Cuts at: 0 316 619 880 1125
Size: 316 303 261 245
Fragments arranged by size:
316 303 261 245
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 783 1125
Size: 783 342
SfoI GGC'GCC
Cuts at: 0 263 537 585 699 939 1125
Size: 263 274 48 114 240 186
Fragments arranged by size:
274 263 240 186 114 48
SmaI CCC'GGG
Cuts at: 0 695 1125
Size: 695 430
SmlI C'TyrA_G
Cuts at: 0 58 1125
Size: 58 1067
SrfI GCCC'GGGC
Cuts at: 0 695 1125
Size: 695 430
StuI AGG'CCT
Cuts at: 0 952 1125
Size: 952 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1099 1125
Size: 1099 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 493 525 1125
Size: 493 32 600
Fragments arranged by size:
600 493 32
XmaI C'CCGG_G
Cuts at: 0 693 1125
Size: 693 432
Enzymes that do cut:
AccI AcuI ApaI ApoI AvaI BanI BanII BbeI
BbvCI BcgI BglI BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaHI BseRI BseYI BsgI BsiEI Bsp1286I BspMI
BsrFI BssHII BssSI BtgI BtgZI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI HaeII KasI MscI MslI
MspA1I NaeI NarI NgoMIV NotI PasI PpuMI PspOMI
SacII SapI SfiI SfoI SmaI SmlI SrfI StuI
TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI
BglII BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI
BsaXI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI
NheI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SnaBI SpeI SphI SspI
StyI SwaI TatI Tth111I XbaI XcmI XhoI XmnI
ZraI