################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BbrPI BbsI BciVI BclI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI KpnI MamI MfeI MflI MluI MroI MseI MunI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SbfI ScaI SexAI SfcI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 598 GTCTAC GT^[AC][GT]AC AcsI 823 GAATTC [GA]^AATT[CT] AfaI 159 GTAC GT^AC ApoI 823 GAATTC [GA]^AATT[CT] AvaI 693 CCCGGG C^[CT]CG[GA]G BbvCI 1032 GCTGAGG CCTCAGC(-5/-2) BcgI 284 GCAAGCTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 97 CTAG C^TAG BlpI 72 GCTCAGC GC^T[ACGT]AGC BpmI 517 CTGGAG CTGGAG(16/14) Bpu1102I 72 GCTCAGC GC^T[ACGT]AGC BsgI 145 CTGCAC GTGCAG(16/14) BsoBI 693 CCCGGG C^[CT]CG[GA]G BssSI 811 CTCGTG CACGAG(-5/-1) CelII 72 GCTCAGC GC^T[ACGT]AGC DpnII 126 GATC ^GATC EcoRI 823 GAATTC G^AATTC HphI 353 TCACC GGTGA(8/7) MaeI 97 CTAG C^TAG MboI 126 GATC ^GATC MslI 423 CATGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 126 GATC ^GATC PpuMI 920 GGGTCCT [GA]G^G[AT]CC[CT] RsaI 159 GTAC GT^AC Sau3AI 126 GATC ^GATC SfiI 776 GGCCCGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 693 CCCGGG CCC^GGG SmlI 58 CTCAAG C^T[CT][GA]AG StuI 950 AGGCCT AGG^CCT TaqI 173 TCGA T^CGA Tsp509I 824 AATT ^AATT TspRI 473 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 173 TCGA T^CGA XmaCI 693 CCCGGG C^CCGGG XmaI 693 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BseRI 808 CTCCTC GAGGAG(10/8) 883 CTCCTC BsmFI 1037 GGGAC GGGAC(10/14) 943 GTCCC BspMI 164 ACCTGC ACCTGC(4/8) 914 ACCTGC DdeI 73 CTCAG C^T[ACGT]AG 1033 CTGAG HgaI 502 GACGC GACGC(5/10) 350 GCGTC MaeII 107 ACGT A^CGT 899 ACGT MaeIII 352 GTCAC ^GT[ACGT]AC 865 GTCAC NaeI 403 GCCGGC GCC^GGC 991 GCCGGC NgoMIV 403 GCCGGC G^CCGGC 991 GCCGGC NlaIII 225 CATG CATG^ 423 CATG Tsp45I 352 GTCAC ^GT[GC]AC 865 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AccII AciI AcyI AluI ApaI AvaII BalI BanI BanII BbeI BbvI BcnI BglI BmyI BsaHI BsaJI BsiEI BsiYI BslI Bsp1286I BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI KasI Ksp632I KspI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIV NotI SacII SapI Sau96I ScrFI SfaNI SfoI TseI