PPM1E ORF clone FHC00730

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00730

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Clone info.

Species

human

Clone Name

pFN21ASDA1072 Sequence (with vector) Restriction Map

Product ID

FHC00730

Flexi Clone PID

FXC00730

ORF Sequence

DNA sequence (2271 bp)
Protein sequence (757 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384101]

Gene

KIAA1072

Symbol

PPM1E

Alias : CAMKPN, CaMKP-N, POPX1, PP2CH, caMKN

Description

protein phosphatase, Mg2+/Mn2+ dependent, 1E, transcript variant 1

Original Clone ID

hj06360

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2271 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 757 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11613.1 1.4e-160 99.7 PPM1E CCDS13796.1 3.8e-31 47.1 PPM1F The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001932 235 483 PF00481 Protein phosphatase 2C-like HMMSmart IPR001932 221 488 SM00332 Protein phosphatase 2C-like Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA1072 Download

>KIAA1072  2271 bp
ATGGCCGGCTGCATCCCTGAGGAGAAAACTTACCGGCGCTTCCTGGAGCTATTCCTGGGC
GAGTTTCGCGGACCGTGCGGCGGCGGCGAGCCGGAGCCGGAACCCGAACCCGAACCCGAA
CCCGAACCCGAACCCGAGTCCGAGCCCGAGCCCGAACCTGAACTGGTAGAAGCTGAGGCG
GCCGAGGCTTCGGTAGAGGAACCCGGGGAGGAGGCGGCCACGGTAGCCGCGACGGAGGAG
GGGGACCAGGAGCAAGACCCGGAGCCCGAGGAGGAGGCGGCGGTTGAGGGTGAGGAGGAG
GAGGAGGGCGCGGCGACGGCGGCGGCAGCCCCGGGGCACTCGGCCGTGCCGCCGCCGCCG
CCCCAGCTGCCGCCTTTGCCCCCGCTCCCGCGACCGCTGTCAGAGCGCATCACCCGCGAG
GAGGTGGAGGGCGAAAGCCTGGACCTGTGCCTGCAGCAGCTCTACAAATATAATTGCCCT
TCCTTTTTGGCTGCTGCTTTAGCCAGAGCCACATCAGATGAAGTCCTTCAGAGTGATCTT
TCTGCACATTATATCCCAAAGGAAACGGATGGCACAGAAGGGACTGTGGAGATTGAGACA
GTGAAATTGGCCCGTTCTGTCTTCAGCAAACTACACGAGATTTGCTGCAGCTGGGTGAAA
GACTTCCCCCTCCGCAGGAGACCCCAGCTTTATTATGAGACATCAATCCATGCCATCAAA
AACATGCGCAGGAAAATGGAGGACAAACATGTCTGCATTCCTGACTTTAATATGCTCTTC
AACCTAGAGGACCAGGAAGAACAAGCTTACTTTGCAGTGTTTGATGGCCATGGGGGAGTA
GATGCTGCTATTTATGCCTCCATTCACCTCCACGTTAACTTAGTCCGCCAGGAGATGTTC
CCCCATGATCCTGCTGAGGCCCTGTGCAGGGCCTTCCGGGTCACTGATGAGCGGTTTGTG
CAGAAAGCAGCCAGGGAGAGCTTAAGATGTGGGACCACAGGAGTGGTGACTTTCATCAGA
GGCAACATGCTACATGTGGCCTGGGTGGGTGATTCCCAGGTTATGCTTGTGAGAAAGGGC
CAAGCTGTTGAACTAATGAAGCCACACAAACCAGACAGAGAGGATGAAAAGCAGAGAATT
GAGGCCCTTGGAGGTTGCGTAGTCTGGTTTGGTGCCTGGAGGGTGAATGGAAGTCTGTCG
GTTTCCAGAGCTATTGGAGATGCTGAACATAAGCCATATATCTGTGGGGATGCAGATTCT
GCCTCCACTGTTCTGGATGGGACCGAAGACTACCTCATTCTGGCCTGTGATGGCTTCTAT
GACACCGTGAACCCTGATGAGGCAGTGAAAGTTGTGTCCGACCACCTGAAAGAGAATAAT
GGAGACAGCAGCATGGTTGCCCACAAATTAGTGGCATCAGCTCGTGATGCTGGGTCAAGT
GATAACATCACGGTTATTGTGGTATTCCTGAGGGACATGAACAAAGCTGTAAATGTTAGT
GAGGAATCAGATTGGACAGAGAACTCTTTTCAAGGAGGGCAAGAAGATGGTGGGGATGAT
AAGGAGAATCATGGAGAGTGCAAACGCCCTTGGCCTCAGCACCAGTGCTCAGCACCAGCC
GACCTAGGCTATGATGGGCGTGTGGATTCATTCACTGATAGAACTAGCCTGAGCCCAGGG
TCCCAAATCAACGTGCTGGAAGACCCAGGCTACCTAGATCTCACACAAATAGAAGCAAGC
AAACCTCACAGTGCCCAGTTTTTGCTACCAGTTGAGATGTTTGGTCCTGGTGCACCAAAG
AAAGCAAATCTTATTAATGAGTTAATGATGGAGAAAAAATCAGTTCAGTCATCATTGCCT
GAATGGAGTGGTGCTGGAGAGTTTCCCACTGCTTTCAATTTGGGTTCAACAGGGGAGCAG
ATATACAGAATGCAGAGCTTGTCTCCTGTCTGTTCAGGGTTGGAAAATGAACAGTTCAAA
TCCCCGGGAAACAGAGTTTCTAGATTGTCTCATTTACGCCACCACTACTCAAAGAAGTGG
CACAGATTCAGGTTTAATCCAAAGTTTTATTCATTTCTCTCTGCTCAAGAGCCTTCCCAC
AAAATAGGCACTAGCCTGTCCTCACTTACTGGAAGTGGGAAGAGAAATAGGATAAGAAGT
TCTCTGCCATGGAGGCAAAATAGTTGGAAAGGGTACAGTGAAAACATGAGGAAGCTCAGA
AAGACTCATGATATTCCATGCCCAGATCTTCCTTGGAGCTATAAAATAGAA

ORF Protein

Cloned ORF protein sequence for pFN21ASDA1072 Download

>KIAA1072  757 aa
MAGCIPEEKTYRRFLELFLGEFRGPCGGGEPEPEPEPEPEPEPEPESEPEPEPELVEAEA
AEASVEEPGEEAATVAATEEGDQEQDPEPEEEAAVEGEEEEEGAATAAAAPGHSAVPPPP
PQLPPLPPLPRPLSERITREEVEGESLDLCLQQLYKYNCPSFLAAALARATSDEVLQSDL
SAHYIPKETDGTEGTVEIETVKLARSVFSKLHEICCSWVKDFPLRRRPQLYYETSIHAIK
NMRRKMEDKHVCIPDFNMLFNLEDQEEQAYFAVFDGHGGVDAAIYASIHLHVNLVRQEMF
PHDPAEALCRAFRVTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDSQVMLVRKG
QAVELMKPHKPDREDEKQRIEALGGCVVWFGAWRVNGSLSVSRAIGDAEHKPYICGDADS
ASTVLDGTEDYLILACDGFYDTVNPDEAVKVVSDHLKENNGDSSMVAHKLVASARDAGSS
DNITVIVVFLRDMNKAVNVSEESDWTENSFQGGQEDGGDDKENHGECKRPWPQHQCSAPA
DLGYDGRVDSFTDRTSLSPGSQINVLEDPGYLDLTQIEASKPHSAQFLLPVEMFGPGAPK
KANLINELMMEKKSVQSSLPEWSGAGEFPTAFNLGSTGEQIYRMQSLSPVCSGLENEQFK
SPGNRVSRLSHLRHHYSKKWHRFRFNPKFYSFLSAQEPSHKIGTSLSSLTGSGKRNRIRS
SLPWRQNSWKGYSENMRKLRKTHDIPCPDLPWSYKIE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA1072 Download

>pFN21ASDA1072  7000 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGCCGGCTGCATCCC
TGAGGAGAAAACTTACCGGCGCTTCCTGGAGCTATTCCTGGGCGAGTTTCGCGGACCGTG
CGGCGGCGGCGAGCCGGAGCCGGAACCCGAACCCGAACCCGAACCCGAACCCGAACCCGA
GTCCGAGCCCGAGCCCGAACCTGAACTGGTAGAAGCTGAGGCGGCCGAGGCTTCGGTAGA
GGAACCCGGGGAGGAGGCGGCCACGGTAGCCGCGACGGAGGAGGGGGACCAGGAGCAAGA
CCCGGAGCCCGAGGAGGAGGCGGCGGTTGAGGGTGAGGAGGAGGAGGAGGGCGCGGCGAC
GGCGGCGGCAGCCCCGGGGCACTCGGCCGTGCCGCCGCCGCCGCCCCAGCTGCCGCCTTT
GCCCCCGCTCCCGCGACCGCTGTCAGAGCGCATCACCCGCGAGGAGGTGGAGGGCGAAAG
CCTGGACCTGTGCCTGCAGCAGCTCTACAAATATAATTGCCCTTCCTTTTTGGCTGCTGC
TTTAGCCAGAGCCACATCAGATGAAGTCCTTCAGAGTGATCTTTCTGCACATTATATCCC
AAAGGAAACGGATGGCACAGAAGGGACTGTGGAGATTGAGACAGTGAAATTGGCCCGTTC
TGTCTTCAGCAAACTACACGAGATTTGCTGCAGCTGGGTGAAAGACTTCCCCCTCCGCAG
GAGACCCCAGCTTTATTATGAGACATCAATCCATGCCATCAAAAACATGCGCAGGAAAAT
GGAGGACAAACATGTCTGCATTCCTGACTTTAATATGCTCTTCAACCTAGAGGACCAGGA
AGAACAAGCTTACTTTGCAGTGTTTGATGGCCATGGGGGAGTAGATGCTGCTATTTATGC
CTCCATTCACCTCCACGTTAACTTAGTCCGCCAGGAGATGTTCCCCCATGATCCTGCTGA
GGCCCTGTGCAGGGCCTTCCGGGTCACTGATGAGCGGTTTGTGCAGAAAGCAGCCAGGGA
GAGCTTAAGATGTGGGACCACAGGAGTGGTGACTTTCATCAGAGGCAACATGCTACATGT
GGCCTGGGTGGGTGATTCCCAGGTTATGCTTGTGAGAAAGGGCCAAGCTGTTGAACTAAT
GAAGCCACACAAACCAGACAGAGAGGATGAAAAGCAGAGAATTGAGGCCCTTGGAGGTTG
CGTAGTCTGGTTTGGTGCCTGGAGGGTGAATGGAAGTCTGTCGGTTTCCAGAGCTATTGG
AGATGCTGAACATAAGCCATATATCTGTGGGGATGCAGATTCTGCCTCCACTGTTCTGGA
TGGGACCGAAGACTACCTCATTCTGGCCTGTGATGGCTTCTATGACACCGTGAACCCTGA
TGAGGCAGTGAAAGTTGTGTCCGACCACCTGAAAGAGAATAATGGAGACAGCAGCATGGT
TGCCCACAAATTAGTGGCATCAGCTCGTGATGCTGGGTCAAGTGATAACATCACGGTTAT
TGTGGTATTCCTGAGGGACATGAACAAAGCTGTAAATGTTAGTGAGGAATCAGATTGGAC
AGAGAACTCTTTTCAAGGAGGGCAAGAAGATGGTGGGGATGATAAGGAGAATCATGGAGA
GTGCAAACGCCCTTGGCCTCAGCACCAGTGCTCAGCACCAGCCGACCTAGGCTATGATGG
GCGTGTGGATTCATTCACTGATAGAACTAGCCTGAGCCCAGGGTCCCAAATCAACGTGCT
GGAAGACCCAGGCTACCTAGATCTCACACAAATAGAAGCAAGCAAACCTCACAGTGCCCA
GTTTTTGCTACCAGTTGAGATGTTTGGTCCTGGTGCACCAAAGAAAGCAAATCTTATTAA
TGAGTTAATGATGGAGAAAAAATCAGTTCAGTCATCATTGCCTGAATGGAGTGGTGCTGG
AGAGTTTCCCACTGCTTTCAATTTGGGTTCAACAGGGGAGCAGATATACAGAATGCAGAG
CTTGTCTCCTGTCTGTTCAGGGTTGGAAAATGAACAGTTCAAATCCCCGGGAAACAGAGT
TTCTAGATTGTCTCATTTACGCCACCACTACTCAAAGAAGTGGCACAGATTCAGGTTTAA
TCCAAAGTTTTATTCATTTCTCTCTGCTCAAGAGCCTTCCCACAAAATAGGCACTAGCCT
GTCCTCACTTACTGGAAGTGGGAAGAGAAATAGGATAAGAAGTTCTCTGCCATGGAGGCA
AAATAGTTGGAAAGGGTACAGTGAAAACATGAGGAAGCTCAGAAAGACTCATGATATTCC
ATGCCCAGATCTTCCTTGGAGCTATAAAATAGAATACGTAGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT