Restriction map for
pFN21ASDA1072
(Linear) (Six-base) MAPSORT of: KIAA1072.seq Check: 8923 from: 1 to: 2271
KIAA1072.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 606 2271
Size: 511 95 1665
Fragments arranged by size:
1665 511 95
AflII C'TTAA_G
Cuts at: 0 981 2271
Size: 981 1290
AflIII A'CryG_T
Cuts at: 0 747 1032 2271
Size: 747 285 1239
Fragments arranged by size:
1239 747 285
AleI CACnn'nnGTG
Cuts at: 0 1000 2271
Size: 1000 1271
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2142 2174 2271
Size: 2142 32 97
Fragments arranged by size:
2142 97 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1258 2271
Size: 1258 1013
ApaLI G'TGCA_C
Cuts at: 0 1790 2271
Size: 1790 481
AseI AT'TA_AT
Cuts at: 0 1814 2271
Size: 1814 457
AvaI C'yCGr_G
Cuts at: 0 133 145 202 265 330 1983 2271
Size: 133 12 57 63 65 1653 288
Fragments arranged by size:
1653 288 133 65 63 57 12
AvrII C'CTAG_G
Cuts at: 0 1623 2271
Size: 1623 648
BanI G'GyrC_C
Cuts at: 0 1171 2271
Size: 1171 1100
BanII G_rGCy'C
Cuts at: 0 146 152 266 1675 2271
Size: 146 6 114 1409 596
Fragments arranged by size:
1409 596 146 114 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 612 1292 1706 2271
Size: 612 680 414 565
Fragments arranged by size:
680 612 565 414
BbvCI CC'TCA_GC
Cuts at: 0 173 914 1595 2271
Size: 173 741 681 676
Fragments arranged by size:
741 681 676 173
BglI GCCn_nnn'nGGC
Cuts at: 0 2172 2271
Size: 2172 99
BglII A'GATC_T
Cuts at: 0 1716 2244 2271
Size: 1716 528 27
Fragments arranged by size:
1716 528 27
BlpI GC'TnA_GC
Cuts at: 0 1608 2271
Size: 1608 663
Bme1580I G_kGCm'C
Cuts at: 0 338 1755 1794 2271
Size: 338 1417 39 477
Fragments arranged by size:
1417 477 338 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 1749 2271
Size: 1749 522
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1958 2271
Size: 1958 313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 1197 1895 2271
Size: 64 1133 698 376
Fragments arranged by size:
1133 698 376 64
Bpu10I CC'TnA_GC
Cuts at: 0 173 914 1595 1669 2271
Size: 173 741 681 74 602
Fragments arranged by size:
741 681 602 173 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2069 2271
Size: 2069 202
BsaI GGTCTCn'nnnn_
Cuts at: 0 672 2271
Size: 672 1599
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1142 1172 2271
Size: 1142 30 1099
Fragments arranged by size:
1142 1099 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 223 250 283 286 307 310 313
Size: 34 189 27 33 3 21 3 3
Cuts at: 313 316 433 2271
Size: 3 117 1838
Fragments arranged by size:
1838 189 117 34 33 27 21 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 362 650 683 1429 2271
Size: 362 288 33 746 842
Fragments arranged by size:
842 746 362 288 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 945 979 2271
Size: 527 418 34 1292
Fragments arranged by size:
1292 527 418 34
BsiEI CG_ry'CG
Cuts at: 0 182 344 394 2271
Size: 182 162 50 1877
Fragments arranged by size:
1877 182 162 50
BsiHKAI G_wGCw'C
Cuts at: 0 1609 1794 2271
Size: 1609 185 477
Fragments arranged by size:
1609 477 185
BsmI GAATG_Cn'
Cuts at: 0 755 1934 2271
Size: 755 1179 337
Fragments arranged by size:
1179 755 337
Bsp1286I G_dGCh'C
Cuts at: 0 146 152 266 338 1609 1675 1755 1794
Size: 146 6 114 72 1271 66 80 39
Cuts at: 1794 2271
Size: 477
Fragments arranged by size:
1271 477 146 114 80 72 66 39
6
BspHI T'CATG_A
Cuts at: 0 2226 2271
Size: 2226 45
BsrBI CCG'CTC
Cuts at: 0 384 951 2271
Size: 384 567 1320
Fragments arranged by size:
1320 567 384
BsrDI GCAATG_nn'
Cuts at: 0 1852 2271
Size: 1852 419
BsrFI r'CCGG_y
Cuts at: 0 4 32 2271
Size: 4 28 2239
Fragments arranged by size:
2239 28 4
BssSI C'ACGA_G
Cuts at: 0 634 1421 2271
Size: 634 787 850
Fragments arranged by size:
850 787 634
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1258 2271
Size: 1258 1013
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1002 1272 2271
Size: 1002 270 999
Fragments arranged by size:
1002 999 270
BstYI r'GATC_y
Cuts at: 0 1716 2244 2271
Size: 1716 528 27
Fragments arranged by size:
1716 528 27
Bsu36I CC'TnA_GG
Cuts at: 0 17 1468 2271
Size: 17 1451 803
Fragments arranged by size:
1451 803 17
BtgI C'CryG_G
Cuts at: 0 218 828 2167 2271
Size: 218 610 1339 104
Fragments arranged by size:
1339 610 218 104
BtsI GCAGTG_nn'
Cuts at: 0 821 1349 1886 2271
Size: 821 528 537 385
Fragments arranged by size:
821 537 528 385
DrdI GACnn_nn'nnGTC
Cuts at: 0 748 2271
Size: 748 1523
EaeI y'GGCC_r
Cuts at: 0 2 179 215 341 825 2271
Size: 2 177 36 126 484 1446
Fragments arranged by size:
1446 484 177 126 36 2
EagI C'GGCC_G
Cuts at: 0 179 341 2271
Size: 179 162 1930
Fragments arranged by size:
1930 179 162
EarI CTCTTCn'nnn_
Cuts at: 0 781 2134 2271
Size: 781 1353 137
Fragments arranged by size:
1353 781 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 874 2271
Size: 874 1397
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 511 606 1197 1895 2271
Size: 64 447 95 591 698 376
Fragments arranged by size:
698 591 447 376 95 64
EcoO109I rG'GnC_Cy
Cuts at: 0 918 929 1143 1679 2271
Size: 918 11 214 536 592
Fragments arranged by size:
918 592 536 214 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1825 2271
Size: 1825 446
FspI TGC'GCA
Cuts at: 0 727 2271
Size: 727 1544
HaeII r_GCGC'y
Cuts at: 0 39 2271
Size: 39 2232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1246 1278 1417 1449 1767 1799 1822 1854
Size: 1246 32 139 32 318 32 23 32
Cuts at: 1854 2271
Size: 417
Fragments arranged by size:
1246 417 318 139 32 32 32 32
23
HincII GTy'rAC
Cuts at: 0 876 2271
Size: 876 1395
HindIII A'AGCT_T
Cuts at: 0 803 2271
Size: 803 1468
HpaI GTT'AAC
Cuts at: 0 876 2271
Size: 876 1395
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1382 1940 2164 2271
Size: 1382 558 224 107
Fragments arranged by size:
1382 558 224 107
MscI TGG'CCA
Cuts at: 0 827 2271
Size: 827 1444
MslI CAynn'nnrTG
Cuts at: 0 1000 2271
Size: 1000 1271
MspA1I CmG'CkG
Cuts at: 0 366 396 650 2271
Size: 366 30 254 1621
Fragments arranged by size:
1621 366 254 30
NaeI GCC'GGC
Cuts at: 0 6 2271
Size: 6 2265
NcoI C'CATG_G
Cuts at: 0 828 2167 2271
Size: 828 1339 104
Fragments arranged by size:
1339 828 104
NgoMIV G'CCGG_C
Cuts at: 0 4 2271
Size: 4 2267
NspI r_CATG'y
Cuts at: 0 726 751 1029 1036 2271
Size: 726 25 278 7 1235
Fragments arranged by size:
1235 726 278 25 7
PasI CC'CwG_GG
Cuts at: 0 1675 2271
Size: 1675 596
PciI A'CATG_T
Cuts at: 0 747 1032 2271
Size: 747 285 1239
Fragments arranged by size:
1239 747 285
PfoI T'CCnGG_A
Cuts at: 0 41 2271
Size: 41 2230
PpuMI rG'GwC_Cy
Cuts at: 0 1679 2271
Size: 1679 592
PshAI GACnn'nnGTC
Cuts at: 0 396 2271
Size: 396 1875
PstI C_TGCA'G
Cuts at: 0 455 649 2271
Size: 455 194 1622
Fragments arranged by size:
1622 455 194
PvuII CAG'CTG
Cuts at: 0 366 650 2271
Size: 366 284 1621
Fragments arranged by size:
1621 366 284
RsrII CG'GwC_CG
Cuts at: 0 70 2271
Size: 70 2201
SanDI GG'GwC_CC
Cuts at: 0 1679 2271
Size: 1679 592
SapI GCTCTTCn'nnn_
Cuts at: 0 781 2271
Size: 781 1490
SfcI C'TryA_G
Cuts at: 0 451 645 2271
Size: 451 194 1626
Fragments arranged by size:
1626 451 194
SmaI CCC'GGG
Cuts at: 0 204 332 1985 2271
Size: 204 128 1653 286
Fragments arranged by size:
1653 286 204 128
SmlI C'TyrA_G
Cuts at: 0 981 2084 2271
Size: 981 1103 187
Fragments arranged by size:
1103 981 187
StyI C'CwwG_G
Cuts at: 0 828 1146 1588 1623 2167 2251 2271
Size: 828 318 442 35 544 84 20
Fragments arranged by size:
828 544 442 318 84 35 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 642 1032 1036 1297 2271
Size: 642 390 4 261 974
Fragments arranged by size:
974 642 390 261 4
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 536 568 2271
Size: 536 32 1703
Fragments arranged by size:
1703 536 32
XbaI T'CTAG_A
Cuts at: 0 1999 2271
Size: 1999 272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2249 2271
Size: 2249 22
XmaI C'CCGG_G
Cuts at: 0 202 330 1983 2271
Size: 202 128 1653 288
Fragments arranged by size:
1653 288 202 128
XmnI GAAnn'nnTTC
Cuts at: 0 524 661 2271
Size: 524 137 1610
Fragments arranged by size:
1610 524 137
Enzymes that do cut:
AcuI AflII AflIII AleI AloI AlwNI ApaLI AseI
AvaI AvrII BanI BanII BbsI BbvCI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtsI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I FalI FspI HaeII Hin4I HincII HindIII
HpaI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PasI PciI PfoI PpuMI PshAI PstI PvuII
RsrII SanDI SapI SfcI SmaI SmlI StyI TaqII
TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI ApaI ApoI AscI AsiSI BaeI BamHI BbeI
BcgI BciVI BclI BfrBI BmgBI BmtI BsaAI BsaBI
BsaHI BsaWI BsiWI BsmBI BspEI BspMI BsrGI BssHII
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
EcoICRI EcoNI EcoRI EcoRV FseI FspAI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
PacI PflMI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI SacI SacII SalI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XhoI ZraI