(Circular) (Six-base) MAPSORT of: pFN21ASDA1072.seq Check: 7788 from: 1 to: 7000 pFN21ASDA1072 7000 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4810 278 Size: 53 83 186 936 3274 2468 Fragments arranged by size: 3274 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4343 1937 Size: 2406 4594 Acc65I G'GTAC_C Cuts at: 4321 4321 Size: 7000 AclI AA'CG_TT Cuts at: 1563 5127 5500 1563 Size: 3564 373 3063 Fragments arranged by size: 3564 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2534 2629 5063 6447 6715 1632 Size: 902 95 2434 1384 268 1917 Fragments arranged by size: 2434 1917 1384 902 268 95 AflII C'TTAA_G Cuts at: 820 1017 3004 820 Size: 197 1987 4816 Fragments arranged by size: 4816 1987 197 AflIII A'CryG_T Cuts at: 1203 2770 3055 5899 6876 1203 Size: 1567 285 2844 977 1327 Fragments arranged by size: 2844 1567 1327 977 285 AgeI A'CCGG_T Cuts at: 5831 5831 Size: 7000 AhdI GACnn_n'nnGTC Cuts at: 5729 5729 Size: 7000 AleI CACnn'nnGTG Cuts at: 1166 3023 1166 Size: 1857 5143 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7000 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4165 4197 4165 Size: 32 6968 AlwNI CAG_nnn'CTG Cuts at: 3281 4743 6315 3281 Size: 1462 1572 3966 Fragments arranged by size: 3966 1572 1462 ApaLI G'TGCA_C Cuts at: 3813 5057 6213 3813 Size: 1244 1156 4600 Fragments arranged by size: 4600 1244 1156 ApoI r'AATT_y Cuts at: 1493 4309 4535 4687 6796 1493 Size: 2816 226 152 2109 1697 Fragments arranged by size: 2816 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3837 5554 160 Size: 872 2805 1717 1606 Fragments arranged by size: 2805 1717 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7000 AvaI C'yCGr_G Cuts at: 1870 1943 2156 2168 2225 2288 2353 4006 4325 Size: 73 213 12 57 63 65 1653 319 Cuts at: 4325 1870 Size: 4545 Fragments arranged by size: 4545 1653 319 213 73 65 63 57 12 AvrII C'CTAG_G Cuts at: 3646 3646 Size: 7000 BamHI G'GATC_C Cuts at: 4330 4697 4330 Size: 367 6633 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3194 4321 5776 618 Size: 325 217 627 1407 1127 1455 1842 Fragments arranged by size: 1842 1455 1407 1127 627 325 217 BanII G_rGCy'C Cuts at: 1391 2169 2175 2289 3698 4319 1391 Size: 778 6 114 1409 621 4072 Fragments arranged by size: 4072 1409 778 621 114 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2635 3315 3729 928 Size: 971 736 680 414 4199 Fragments arranged by size: 4199 971 736 680 414 BbvCI CC'TCA_GC Cuts at: 2196 2937 3618 2196 Size: 741 681 5578 Fragments arranged by size: 5578 741 681 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5191 5225 1903 Size: 34 3254 34 3678 Fragments arranged by size: 3678 3254 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4892 6102 4892 Size: 1210 5790 BclI T'GATC_A Cuts at: 1548 1548 Size: 7000 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4195 5611 136 Size: 107 122 71 631 605 2523 1416 1525 Fragments arranged by size: 2523 1525 1416 631 605 122 107 71 BglII A'GATC_T Cuts at: 3739 4267 6995 3739 Size: 528 2728 3744 Fragments arranged by size: 3744 2728 528 BlpI GC'TnA_GC Cuts at: 3631 4417 3631 Size: 786 6214 Bme1580I G_kGCm'C Cuts at: 1790 2361 3778 3817 5061 6217 1790 Size: 571 1417 39 1244 1156 2573 Fragments arranged by size: 2573 1417 1244 1156 571 39 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7000 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3772 4731 5689 Size: 416 53 83 399 24 2355 959 958 Cuts at: 5689 442 Size: 1753 Fragments arranged by size: 2355 1753 959 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7000 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3981 3981 Size: 7000 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 3220 3918 5660 1239 Size: 848 1133 698 1742 2579 Fragments arranged by size: 2579 1742 1133 848 698 Bpu10I CC'TnA_GC Cuts at: 2196 2937 3618 3692 2196 Size: 741 681 74 5504 Fragments arranged by size: 5504 741 681 74 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4092 5125 5990 6288 4092 Size: 1033 865 298 4804 Fragments arranged by size: 4804 1033 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2695 5663 882 Size: 617 1196 2968 2219 Fragments arranged by size: 2968 2219 1196 617 BsaAI yAC'GTr Cuts at: 493 1204 4297 4791 493 Size: 711 3093 494 2702 Fragments arranged by size: 3093 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6994 6994 Size: 7000 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4807 5189 5824 275 Size: 53 83 186 936 3274 382 635 1451 Fragments arranged by size: 3274 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5433 5831 6105 6252 6663 5433 Size: 398 274 147 411 5770 Fragments arranged by size: 5770 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3165 3195 1178 Size: 30 165 30 1762 30 4983 Fragments arranged by size: 4983 1762 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2246 2273 2306 2309 2330 2333 2336 Size: 871 189 27 33 3 21 3 3 Cuts at: 2336 2339 2456 1186 Size: 3 117 5730 Fragments arranged by size: 5730 871 189 117 33 27 21 3 3 3 3 BseYI C'CCAG_C Cuts at: 2385 2673 2706 3452 6203 6699 2385 Size: 288 33 746 2751 496 2686 Fragments arranged by size: 2751 2686 746 496 288 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2550 2968 3002 1163 Size: 573 814 418 34 5161 Fragments arranged by size: 5161 814 573 418 34 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2205 2367 2417 4450 5211 6239 Size: 275 186 204 162 50 2033 761 1028 Cuts at: 6239 1540 Size: 2301 Fragments arranged by size: 2301 2033 1028 761 275 204 186 162 50 BsiHKAI G_wGCw'C Cuts at: 3632 3817 5061 5146 6217 3632 Size: 185 1244 85 1071 4415 Fragments arranged by size: 4415 1244 1071 185 85 BsmI GAATG_Cn' Cuts at: 2778 3957 4511 4604 2778 Size: 1179 554 93 5174 Fragments arranged by size: 5174 1179 554 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2175 2289 2361 3632 3698 3778 Size: 399 379 6 114 72 1271 66 80 Cuts at: 3778 3817 4319 5061 5146 6217 1391 Size: 39 502 742 85 1071 2174 Fragments arranged by size: 2174 1271 1071 742 502 399 379 114 85 80 72 66 39 6 BspEI T'CCGG_A Cuts at: 6663 6663 Size: 7000 BspHI T'CATG_A Cuts at: 4249 4889 6619 4249 Size: 640 1730 4630 Fragments arranged by size: 4630 1730 640 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4355 844 Size: 3511 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2407 2974 4887 1394 Size: 429 584 567 1913 3507 Fragments arranged by size: 3507 1913 584 567 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3875 5495 5669 65 Size: 1183 2627 1620 174 1396 Fragments arranged by size: 2627 1620 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2027 2055 5644 5831 1700 Size: 110 217 28 3589 187 2869 Fragments arranged by size: 3589 2869 217 187 110 28 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7000 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7000 BssSI C'ACGA_G Cuts at: 2657 3444 5060 6072 2657 Size: 787 1616 1012 3585 Fragments arranged by size: 3585 1616 1012 787 BstAPI GCAn_nnn'nTGC Cuts at: 794 3281 794 Size: 2487 4513 BstBI TT'CG_AA Cuts at: 2007 5804 2007 Size: 3797 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7000 BstXI CCAn_nnnn'nTGG Cuts at: 3025 3295 3025 Size: 270 6730 BstYI r'GATC_y Cuts at: 1972 3739 4267 4330 4697 5082 5099 6551 6637 Size: 1767 528 63 367 385 17 1452 86 Cuts at: 6637 6649 6995 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1767 1452 528 385 367 346 86 63 17 12 Bsu36I CC'TnA_GG Cuts at: 2040 3491 2040 Size: 1451 5549 BtgI C'CryG_G Cuts at: 513 1065 2022 2241 2851 4190 513 Size: 552 957 219 610 1339 3323 Fragments arranged by size: 3323 1339 957 610 552 219 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6261 Fragments arranged by size: 6261 683 56 BtsI GCAGTG_nn' Cuts at: 2844 3372 3909 4516 5310 5330 2844 Size: 528 537 607 794 20 4514 Fragments arranged by size: 4514 794 607 537 528 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7000 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6965 DraI TTT'AAA Cuts at: 4304 4304 Size: 7000 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2771 6007 809 Size: 507 1455 3236 1802 Fragments arranged by size: 3236 1802 1455 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2202 2238 Size: 54 1425 50 275 18 195 177 36 Cuts at: 2238 2364 2848 4447 5336 6965 8 Size: 126 484 1599 889 1629 43 Fragments arranged by size: 1629 1599 1425 889 484 275 195 177 126 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 2202 2364 4447 1537 Size: 275 390 162 2083 4090 Fragments arranged by size: 4090 2083 390 275 162 EarI CTCTTCn'nnn_ Cuts at: 1353 2804 4157 4930 1353 Size: 1451 1353 773 3423 Fragments arranged by size: 3423 1451 1353 773 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2897 5587 5961 6107 387 Size: 1064 138 1308 2690 374 146 1280 Fragments arranged by size: 2690 1308 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2534 2629 3220 3918 5063 5660 Size: 393 455 447 95 591 698 1145 597 Cuts at: 5660 6447 6715 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1145 787 698 597 591 455 447 393 268 95 EcoO109I rG'GnC_Cy Cuts at: 2941 2952 3166 3702 2941 Size: 11 214 536 6239 Fragments arranged by size: 6239 536 214 11 EcoRI G'AATT_C Cuts at: 4309 4687 4309 Size: 378 6622 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3848 3848 Size: 7000 FspI TGC'GCA Cuts at: 2750 5506 2750 Size: 2756 4244 HaeII r_GCGC'y Cuts at: 1678 2062 6147 1678 Size: 384 4085 2531 Fragments arranged by size: 4085 2531 384 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3269 3301 3440 3472 3790 Size: 32 135 32 1697 32 139 32 318 Cuts at: 3790 3822 3845 3877 5641 5673 5715 5747 1373 Size: 32 23 32 1764 32 42 32 2626 Fragments arranged by size: 2626 1764 1697 318 139 135 42 32 32 32 32 32 32 32 32 23 HincII GTy'rAC Cuts at: 669 1657 1938 2899 4344 4590 669 Size: 988 281 961 1445 246 3079 Fragments arranged by size: 3079 1445 988 961 281 246 HindIII A'AGCT_T Cuts at: 748 2826 748 Size: 2078 4922 HpaI GTT'AAC Cuts at: 2899 4590 2899 Size: 1691 5309 KpnI G_GTAC'C Cuts at: 4325 4325 Size: 7000 MfeI C'AATT_G Cuts at: 4599 4599 Size: 7000 MluI A'CGCG_T Cuts at: 6876 6876 Size: 7000 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3405 3963 4187 6114 6298 1305 Size: 2100 558 224 1927 184 2007 Fragments arranged by size: 2100 2007 1927 558 224 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2850 10 Size: 54 1425 343 1018 4160 Fragments arranged by size: 4160 1425 1018 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3023 4958 5317 5476 518 Size: 648 1857 1935 359 159 2042 Fragments arranged by size: 2042 1935 1857 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2389 2419 2673 4416 5093 6241 6486 1606 Size: 783 30 254 1743 677 1148 245 2120 Fragments arranged by size: 2120 1743 1148 783 677 254 245 30 NaeI GCC'GGC Cuts at: 2029 2029 Size: 7000 NcoI C'CATG_G Cuts at: 513 1065 2022 2851 4190 513 Size: 552 957 829 1339 3323 Fragments arranged by size: 3323 1339 957 829 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7000 NgoMIV G'CCGG_C Cuts at: 2027 2027 Size: 7000 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7000 NotI GC'GGCC_GC Cuts at: 4447 4447 Size: 7000 NspI r_CATG'y Cuts at: 1132 1229 2749 2774 3052 3059 4358 5903 1132 Size: 97 1520 25 278 7 1299 1545 2229 Fragments arranged by size: 2229 1545 1520 1299 278 97 25 7 PasI CC'CwG_GG Cuts at: 1348 3698 1348 Size: 2350 4650 PciI A'CATG_T Cuts at: 2770 3055 5899 2770 Size: 285 2844 3871 Fragments arranged by size: 3871 2844 285 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7000 PfoI T'CCnGG_A Cuts at: 2064 2064 Size: 7000 PmeI GTTT'AAAC Cuts at: 4304 4304 Size: 7000 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5069 5101 6582 6614 5069 Size: 32 1481 32 5455 Fragments arranged by size: 5455 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3702 3702 Size: 7000 PshAI GACnn'nnGTC Cuts at: 1613 2419 1613 Size: 806 6194 PsiI TTA'TAA Cuts at: 4570 4570 Size: 7000 PstI C_TGCA'G Cuts at: 830 2478 2672 4352 830 Size: 1648 194 1680 3478 Fragments arranged by size: 3478 1680 1648 194 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7000 PvuII CAG'CTG Cuts at: 2389 2673 2389 Size: 284 6716 RsrII CG'GwC_CG Cuts at: 2093 2093 Size: 7000 SalI G'TCGA_C Cuts at: 1936 4342 1936 Size: 2406 4594 SanDI GG'GwC_CC Cuts at: 3702 3702 Size: 7000 SapI GCTCTTCn'nnn_ Cuts at: 2804 2804 Size: 7000 SbfI CC_TGCA'GG Cuts at: 4352 4352 Size: 7000 ScaI AGT'ACT Cuts at: 5248 6960 5248 Size: 1712 5288 SfcI C'TryA_G Cuts at: 826 1045 2474 2668 4348 5483 6164 6355 826 Size: 219 1429 194 1680 1135 681 191 1471 Fragments arranged by size: 1680 1471 1429 1135 681 219 194 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7000 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7000 SmaI CCC'GGG Cuts at: 1872 2227 2355 4008 4327 1872 Size: 355 128 1653 319 4545 Fragments arranged by size: 4545 1653 355 319 128 SmlI C'TyrA_G Cuts at: 820 1017 1943 3004 4107 5104 6005 6267 820 Size: 197 926 1061 1103 997 901 262 1553 Fragments arranged by size: 1553 1103 1061 997 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4297 4791 493 Size: 3804 494 2702 Fragments arranged by size: 3804 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7000 SphI G_CATG'C Cuts at: 1132 4358 1132 Size: 3226 3774 SspI AAT'ATT Cuts at: 5 52 4924 5 Size: 47 4872 2081 Fragments arranged by size: 4872 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7000 StyI C'CwwG_G Cuts at: 513 1065 2022 2851 3169 3611 3646 4190 4274 Size: 552 957 829 318 442 35 544 84 Cuts at: 4274 4439 513 Size: 165 3074 Fragments arranged by size: 3074 957 829 552 544 442 318 165 84 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2665 3055 3059 3320 5027 5044 5197 Size: 1255 1085 390 4 261 1707 17 153 Cuts at: 5197 5382 5827 325 Size: 185 445 1498 Fragments arranged by size: 1707 1498 1255 1085 445 390 261 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5246 6958 96 Size: 274 80 33 51 1443 3269 1712 138 Fragments arranged by size: 3269 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2559 2591 6960 6992 2559 Size: 32 4369 32 2567 Fragments arranged by size: 4369 2567 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7000 XbaI T'CTAG_A Cuts at: 4022 4336 4022 Size: 314 6686 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4272 4272 Size: 7000 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7000 XmaI C'CCGG_G Cuts at: 1870 2225 2353 4006 4325 1870 Size: 355 128 1653 319 4545 Fragments arranged by size: 4545 1653 355 319 128 XmnI GAAnn'nnTTC Cuts at: 2547 2684 5129 2547 Size: 137 2445 4418 Fragments arranged by size: 4418 2445 137 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4808 276 Size: 53 83 186 936 3274 2468 Fragments arranged by size: 3274 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI SacI SacII SexAI SfiI SfoI SrfI SwaI