Restriction map for
pFN21ASDA1072
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AgeI AhdI Aor51HI
ApaI ApoI AscI Asp718I AspEI
AspI BamHI BbeI BbrPI BcgI
BciVI BclI BsaAI BsaBI BsaHI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspMI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI DraI DraIII Eam1105I Eco47III
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HgaI Hin1I KasI
KpnI KspI MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI Psp1406I PvuI SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AfaI 2193 GTAC GT^AC
AflII 981 CTTAAG C^TTAAG
Alw44I 1790 GTGCAC G^TGCAC
AlwI 907 GATCC GGATC(4/5)
AlwNI 1253 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1790 GTGCAC G^TGCAC
AseI 1813 ATTAAT AT^TAAT
AsnI 1813 ATTAAT AT^TAAT
AviII 725 TGCGCA TGC^GCA
AvrII 1623 CCTAGG C^CTAGG
BalI 825 TGGCCA TGG^CCA
BanI 1171 GGTGCC G^G[CT][GA]CC
BfrI 981 CTTAAG C^TTAAG
BglI 2166 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1623 CCTAGG C^CTAGG
BlpI 1607 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1607 GCTCAGC GC^T[ACGT]AGC
BsaI 678 GAGACC GGTCTC(1/5)
BspHI 2226 TCATGA T^CATGA
BsrDI 1853 CATTGC GCAATG(2/0)
BstAPI 1252 GCAGATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1607 GCTCAGC GC^T[ACGT]AGC
CpoI 69 CGGACCG CG^G[AT]CCG
DrdI 742 GACAAACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 725 TGCGCA TGC^GCA
HaeII 35 GGCGCT [GA]GCGC^[CT]
HincII 874 GTTAAC GT[CT]^[GA]AC
HindII 874 GTTAAC GT[CT]^[GA]AC
HindIII 803 AAGCTT A^AGCTT
HpaI 874 GTTAAC GTT^AAC
MluNI 825 TGGCCA TGG^CCA
MscI 825 TGGCCA TGG^CCA
MslI 996 CACAGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 4 GCCGGC GCC^GGC
NgoMIV 4 GCCGGC G^CCGGC
PpuMI 1678 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 392 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1813 ATTAAT AT^TAAT
RcaI 2226 TCATGA T^CATGA
RsaI 2193 GTAC GT^AC
RsrII 69 CGGACCG CG^G[AT]CCG
SapI 774 GCTCTTC GCTCTTC(1/4)
XbaI 1999 TCTAGA T^CTAGA
XcmI 2242 CCAGATCTTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
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AflIII 747 ACATGT A^C[GA][CT]GT
1032 ACATGT
AocI 16 CCTGAGG CC^T[ACGT]AGG
1467 CCTGAGG
Asp700I 520 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
657 GAAAGACTTC
AspHI 1605 GTGCTC G[AT]GC[AT]^C
1790 GTGCAC
BglII 1716 AGATCT A^GATCT
2244 AGATCT
BsiHKAI 1605 GTGCTC G[AT]GC[AT]^C
1790 GTGCAC
BsmI 1928 GAATGC GAATGC(1/-1)
755 GCATTC
BspLU11I 747 ACATGT A^CATGT
1032 ACATGT
BsrBI 382 CCGCTC CCGCTC(-3/-3)
949 GAGCGG
BsrFI 4 GCCGGC [GA]^CCGG[CT]
32 ACCGGC
BssSI 634 CACGAG CACGAG(-5/-1)
1421 CTCGTG
BstXI 995 CCACAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1265 CCACTGTTCTGG
BstYI 1716 AGATCT [GA]^GATC[CT]
2244 AGATCT
Bsu36I 16 CCTGAGG CC^T[ACGT]AGG
1467 CCTGAGG
Cfr10I 4 GCCGGC [GA]^CCGG[CT]
32 ACCGGC
EagI 179 CGGCCG C^GGCCG
341 CGGCCG
EarI 775 CTCTTC CTCTTC(1/4)
2139 GAAGAG
EclXI 179 CGGCCG C^GGCCG
341 CGGCCG
Eco52I 179 CGGCCG C^GGCCG
341 CGGCCG
Eco81I 16 CCTGAGG CC^T[ACGT]AGG
1467 CCTGAGG
Ksp632I 775 CTCTTC CTCTTC(1/4)
2139 GAAGAG
MaeII 872 ACGT A^CGT
1691 ACGT
MaeIII 940 GTCAC ^GT[ACGT]AC
1006 GTGAC
MflI 1716 AGATCT [GA]^GATC[CT]
2244 AGATCT
NcoI 828 CCATGG C^CATGG
2167 CCATGG
PleI 2223 GACTC GAGTC(4/5)
136 GAGTC
PstI 451 CTGCAG CTGCA^G
645 CTGCAG
PvuII 364 CAGCTG CAG^CTG
648 CAGCTG
SfcI 451 CTGCAG C^T[GA][CT]AG
645 CTGCAG
SmlI 981 CTTAAG C^T[CT][GA]AG
2084 CTCAAG
Tsp45I 940 GTCAC ^GT[GC]AC
1006 GTGAC
XhoII 1716 AGATCT [GA]^GATC[CT]
2244 AGATCT
XmnI 520 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
657 GAAAGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiEI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MboI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI Sau3AI Sau96I ScrFI SfaNI
SmaI StyI TfiI Tru9I TseI
Tsp509I TspRI XmaCI XmaI