AKAP10 ORF clone FHC08876

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Halotag ORF Clone FHC08876

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Clone info.

Species

human

Clone Name

pFN21AE2233 Sequence (with vector) Restriction Map

Product ID

FHC08876

ORF Sequence

DNA sequence (1986 bp)
Protein sequence (662 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590263]

Gene

KIEE2233

Symbol

AKAP10

Alias : AKAP-10, D-AKAP-2, D-AKAP2, PRKA10

Description

A kinase (PRKA) anchor protein 10

Original Clone ID

cs03333

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1986 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 662 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11214.1 0 100.0 AKAP10 CCDS82091.1 1.8e-194 91.2 AKAP10 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000342 291 367 PF00615 Regulator of G protein signalling IPR000342 386 504 PF00615 Regulator of G protein signalling HMMSmart IPR000342 125 369 SM00315 Regulator of G protein signalling IPR000342 379 505 SM00315 Regulator of G protein signalling ProfileScan IPR000342 125 369 PS50132 Regulator of G protein signalling IPR000342 379 505 PS50132 Regulator of G protein signalling
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_009133 662 115080,604694 100.0 100.0 XP_016879560 644 115080,604694 100.0 92.3 NP_001317081 604 115080,604694 91.2 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2233 Download

>KIEE2233  1986 bp
ATGAGGGGAGCCGGGCCCTCCCCGCGCCAGTCCCCCCGCACCCTCCGTCCCGACCCGGGC
CCCGCCATGTCCTTCTTCCGGCGGAAAGTGAAAGGCAAAGAACAAGAGAAGACCTCAGAT
GTGAAGTCCATTAAAGCTTCAATATCCGTACATTCCCCACAAAAAAGCACTAAAAATCAT
GCCTTGCTGGAGGCTGCAGGACCAAGTCATGTTGCAATCAATGCCATTTCTGCCAACATG
GACTCCTTTTCAAGTAGCAGGACAGCCACACTTAAGAAGCAGCCAAGCCACATGGAGGCC
GCTCATTTTGGTGACCTGGGCAGATCTTGTCTGGACTACCAGACTCAAGAGACCAAATCA
AGCCTTTCTAAGACCCTTGAACAAGTCTTGCACGACACTATTGTCCTCCCTTACTTCATT
CAATTCATGGAACTTCGGCGAATGGAGCATTTGGTGAAATTTTGGTTAGAGGCTGAAAGT
TTTCATTCAACAACTTGGTCGCGAATAAGAGCACACAGTCTAAACACAGTGAAGCAGAGC
TCACTGGCTGAGCCTGTCTCTCCATCTAAAAAGCATGAAACTACAGCGTCTTTTTTAACT
GATTCTCTTGATAAGAGATTGGAGGATTCTGGCTCAGCACAGTTGTTTATGACTCATTCA
GAAGGAATTGACCTGAATAATAGAACTAACAGCACTCAGAATCACTTGCTGCTTTCCCAG
GAATGTGACAGTGCCCATTCTCTCCGTCTTGAAATGGCCAGAGCAGGAACTCACCAAGTT
TCCATGGAAACCCAAGAATCTTCCTCTACACTTACAGTAGCCAGTAGAAATAGTCCCGCT
TCTCCACTAAAAGAATTGTCAGGAAAACTAATGAAAAGTATAGAACAAGATGCAGTGAAT
ACTTTTACCAAATATATATCTCCAGATGCTGCTAAACCAATACCAATTACAGAAGCAATG
AGAAATGACATCATAGCAAGGATTTGTGGAGAAGATGGACAGGTGGATCCCAACTGTTTC
GTTTTGGCACAGTCCATAGTCTTTAGTGCAATGGAGCAAGAGCACTTTAGTGAGTTTCTG
CGAAGTCACCATTTCTGTAAATACCAGATTGAAGTGCTGACCAGTGGAACTGTTTACCTG
GCTGACATTCTCTTCTGTGAGTCAGCCCTCTTTTATTTCTCTGAGTACATGGAAAAAGAG
GATGCAGTGAATATCTTACAATTCTGGTTGGCAGCAGATAACTTCCAGTCTCAGCTTGCT
GCCAAAAAGGGCCAATATGATGGACAGGAGGCACAGAATGATGCCATGATTTTATATGAC
AAGTACTTCTCCCTCCAAGCCACACATCCTCTTGGATTTGATGATGTTGTACGATTAGAA
ATTGAATCCAATATCTGCAGGGAAGGTGGGCCACTCCCCAACTGTTTCACAACTCCATTA
CGTCAGGCCTGGACAACCATGGAGAAGGTCTTTTTGCCTGGCTTTTTGTCCAGCAATCTT
TATTATAAATATTTGAATGATCTCATCCATTCGGTTCGAGGAGATGAATTTCTGGGCGGG
AACGTGTCGCTGACTGCTCCTGGCTCTGTTGGCCCTCCTGATGAGTCTCACCCAGGGAGT
TCTGACAGCTCTGCGTCTCAGTCCAGTGTGAAAAAAGCCAGTATTAAAATACTGAAAAAT
TTTGATGAAGCGATAATTGTGGATGCGGCAAGTCTGGATCCAGAATCTTTATATCAACGG
ACATATGCCGGGAAGATGACATTTGGAAGAGTCAGTGACTTGGGGCAATTCATCCGAGAA
TCTGAGCCTGAACCTGATGTAAGGAAATCAAAAGGATCCATGTTCTCACAAGCTATGAAG
AAATGGGTGCAAGGAAATACTGATGAGGCCCAGGAAGAGCTAGCTTGGAAGATTGCTAAA
ATGATAGTCAGTGACATTATGCAGCAGGCTCAGTATGATCAACCGTTAGAGAAATCTACA
AAGTTA

ORF Protein

Cloned ORF protein sequence for pFN21AE2233 Download

>KIEE2233  662 aa
MRGAGPSPRQSPRTLRPDPGPAMSFFRRKVKGKEQEKTSDVKSIKASISVHSPQKSTKNH
ALLEAAGPSHVAINAISANMDSFSSSRTATLKKQPSHMEAAHFGDLGRSCLDYQTQETKS
SLSKTLEQVLHDTIVLPYFIQFMELRRMEHLVKFWLEAESFHSTTWSRIRAHSLNTVKQS
SLAEPVSPSKKHETTASFLTDSLDKRLEDSGSAQLFMTHSEGIDLNNRTNSTQNHLLLSQ
ECDSAHSLRLEMARAGTHQVSMETQESSSTLTVASRNSPASPLKELSGKLMKSIEQDAVN
TFTKYISPDAAKPIPITEAMRNDIIARICGEDGQVDPNCFVLAQSIVFSAMEQEHFSEFL
RSHHFCKYQIEVLTSGTVYLADILFCESALFYFSEYMEKEDAVNILQFWLAADNFQSQLA
AKKGQYDGQEAQNDAMILYDKYFSLQATHPLGFDDVVRLEIESNICREGGPLPNCFTTPL
RQAWTTMEKVFLPGFLSSNLYYKYLNDLIHSVRGDEFLGGNVSLTAPGSVGPPDESHPGS
SDSSASQSSVKKASIKILKNFDEAIIVDAASLDPESLYQRTYAGKMTFGRVSDLGQFIRE
SEPEPDVRKSKGSMFSQAMKKWVQGNTDEAQEELAWKIAKMIVSDIMQQAQYDQPLEKST
KL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2233 Download

>pFN21AE2233  6691 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGGGGAGCCGGGCCCTCCCCGCGCCAGTCCCC
CCGCACCCTCCGTCCCGACCCGGGCCCCGCCATGTCCTTCTTCCGGCGGAAAGTGAAAGG
CAAAGAACAAGAGAAGACCTCAGATGTGAAGTCCATTAAAGCTTCAATATCCGTACATTC
CCCACAAAAAAGCACTAAAAATCATGCCTTGCTGGAGGCTGCAGGACCAAGTCATGTTGC
AATCAATGCCATTTCTGCCAACATGGACTCCTTTTCAAGTAGCAGGACAGCCACACTTAA
GAAGCAGCCAAGCCACATGGAGGCCGCTCATTTTGGTGACCTGGGCAGATCTTGTCTGGA
CTACCAGACTCAAGAGACCAAATCAAGCCTTTCTAAGACCCTTGAACAAGTCTTGCACGA
CACTATTGTCCTCCCTTACTTCATTCAATTCATGGAACTTCGGCGAATGGAGCATTTGGT
GAAATTTTGGTTAGAGGCTGAAAGTTTTCATTCAACAACTTGGTCGCGAATAAGAGCACA
CAGTCTAAACACAGTGAAGCAGAGCTCACTGGCTGAGCCTGTCTCTCCATCTAAAAAGCA
TGAAACTACAGCGTCTTTTTTAACTGATTCTCTTGATAAGAGATTGGAGGATTCTGGCTC
AGCACAGTTGTTTATGACTCATTCAGAAGGAATTGACCTGAATAATAGAACTAACAGCAC
TCAGAATCACTTGCTGCTTTCCCAGGAATGTGACAGTGCCCATTCTCTCCGTCTTGAAAT
GGCCAGAGCAGGAACTCACCAAGTTTCCATGGAAACCCAAGAATCTTCCTCTACACTTAC
AGTAGCCAGTAGAAATAGTCCCGCTTCTCCACTAAAAGAATTGTCAGGAAAACTAATGAA
AAGTATAGAACAAGATGCAGTGAATACTTTTACCAAATATATATCTCCAGATGCTGCTAA
ACCAATACCAATTACAGAAGCAATGAGAAATGACATCATAGCAAGGATTTGTGGAGAAGA
TGGACAGGTGGATCCCAACTGTTTCGTTTTGGCACAGTCCATAGTCTTTAGTGCAATGGA
GCAAGAGCACTTTAGTGAGTTTCTGCGAAGTCACCATTTCTGTAAATACCAGATTGAAGT
GCTGACCAGTGGAACTGTTTACCTGGCTGACATTCTCTTCTGTGAGTCAGCCCTCTTTTA
TTTCTCTGAGTACATGGAAAAAGAGGATGCAGTGAATATCTTACAATTCTGGTTGGCAGC
AGATAACTTCCAGTCTCAGCTTGCTGCCAAAAAGGGCCAATATGATGGACAGGAGGCACA
GAATGATGCCATGATTTTATATGACAAGTACTTCTCCCTCCAAGCCACACATCCTCTTGG
ATTTGATGATGTTGTACGATTAGAAATTGAATCCAATATCTGCAGGGAAGGTGGGCCACT
CCCCAACTGTTTCACAACTCCATTACGTCAGGCCTGGACAACCATGGAGAAGGTCTTTTT
GCCTGGCTTTTTGTCCAGCAATCTTTATTATAAATATTTGAATGATCTCATCCATTCGGT
TCGAGGAGATGAATTTCTGGGCGGGAACGTGTCGCTGACTGCTCCTGGCTCTGTTGGCCC
TCCTGATGAGTCTCACCCAGGGAGTTCTGACAGCTCTGCGTCTCAGTCCAGTGTGAAAAA
AGCCAGTATTAAAATACTGAAAAATTTTGATGAAGCGATAATTGTGGATGCGGCAAGTCT
GGATCCAGAATCTTTATATCAACGGACATATGCCGGGAAGATGACATTTGGAAGAGTCAG
TGACTTGGGGCAATTCATCCGAGAATCTGAGCCTGAACCTGATGTAAGGAAATCAAAAGG
ATCCATGTTCTCACAAGCTATGAAGAAATGGGTGCAAGGAAATACTGATGAGGCCCAGGA
AGAGCTAGCTTGGAAGATTGCTAAAATGATAGTCAGTGACATTATGCAGCAGGCTCAGTA
TGATCAACCGTTAGAGAAATCTACAAAGTTAGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT