Restriction map for pFN21AE2233
 (Circular) (Six-base) MAPSORT of: pFN21AE2233.seq  Check: 1998  from: 1  to: 6691



pFN21AE2233  6691 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:44  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4501     278 
   Size:         53      83     186     936    2965    2468

  Fragments arranged by size: 

                2965    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    4034    1937 
   Size:       2097    4594
 
Acc65I G'GTAC_C

Cuts at:   4012    4012 
   Size:       6691
 
AclI AA'CG_TT

Cuts at:   1563    4818    5191    1563 
   Size:       3255     373    3063

  Fragments arranged by size: 

                3255    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    4754    6138    6406    1632 
   Size:       3122    1384     268    1917

  Fragments arranged by size: 

                3122    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017    2276     820 
   Size:        197    1259    5235

  Fragments arranged by size: 

                5235    1259     197
 
AflIII A'CryG_T

Cuts at:   1203    3567    5590    6567    1203 
   Size:       2364    2023     977    1327

  Fragments arranged by size: 

                2364    2023    1327     977
 
AgeI A'CCGG_T

Cuts at:   5522    5522 
   Size:       6691
 
AhdI GACnn_n'nnGTC

Cuts at:   5420    5420 
   Size:       6691
 
AleI CACnn'nnGTG

Cuts at:   1166    3072    1166 
   Size:       1906    4785
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6691
 
AlwNI CAG_nnn'CTG

Cuts at:   2933    4434    6006    2933 
   Size:       1501    1572    3618

  Fragments arranged by size: 

                3618    1572    1501
 
ApaI G_GGCC'C

Cuts at:   2022    2066    2022 
   Size:         44    6647
 
ApaLI G'TGCA_C

Cuts at:   4748    5904    4748 
   Size:       1156    5535
 
ApoI r'AATT_y

Cuts at:   1493    2462    3551    3682    4000    4226    4378    6487    1493 
   Size:        969    1089     131     318     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1089     969     318     226     152     131
            
 
AseI AT'TA_AT

Cuts at:    160    1032    5245     160 
   Size:        872    4213    1606

  Fragments arranged by size: 

                4213    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6691
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2059    4016    1870 
   Size:         73     116    1957    4545

  Fragments arranged by size: 

                4545    1957     116      73
 
BamHI G'GATC_C

Cuts at:   3010    3721    3839    4021    4388    3010 
   Size:        711     118     182     367    5313

  Fragments arranged by size: 

                5313     711     367     182     118
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    4012    5467     618 
   Size:        325     217     627    2225    1455    1842

  Fragments arranged by size: 

                2225    1842    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2022    2066    2546    4010    1391 
   Size:        631      44     480    1464    4072

  Fragments arranged by size: 

                4072    1464     631     480      44
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2120     928 
   Size:        971     221    5499

  Fragments arranged by size: 

                5499     971     221
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4882    4916    1903 
   Size:         34    2945      34    3678

  Fragments arranged by size: 

                3678    2945      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4583    5793    4583 
   Size:       1210    5481
 
BclI T'GATC_A

Cuts at:   1548    3961    1548 
   Size:       2413    4278
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    5302     136 
   Size:        107     122      71     631     605    3630    1525

  Fragments arranged by size: 

                3630    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2327    6686    2327 
   Size:       4359    2332
 
BlpI GC'TnA_GC

Cuts at:   2553    2638    4108    2553 
   Size:         85    1470    5136

  Fragments arranged by size: 

                5136    1470      85
 
Bme1580I G_kGCm'C

Cuts at:   1790    2022    2066    2740    4752    5908    1790 
   Size:        232      44     674    2012    1156    2573

  Fragments arranged by size: 

                2573    2012    1156     674     232      44
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6691
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4422    5380     442 
   Size:        416      53      83     399      24    3005     958    1753

  Fragments arranged by size: 

                3005    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    3908    1056 
   Size:       2852    3839
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2134    2578    2134 
   Size:        444    6247
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2213    2910    5351    1239 
   Size:        974     697    2441    2579

  Fragments arranged by size: 

                2579    2441     974     697
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2334    4816    5681    5979    2334 
   Size:       2482     865     298    3046

  Fragments arranged by size: 

                3046    2482     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2348    5354     882 
   Size:        617     849    3006    2219

  Fragments arranged by size: 

                3006    2219     849     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4482     493 
   Size:        711    3278    2702

  Fragments arranged by size: 

                3278    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6685    6685 
   Size:       6691
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4498    4880    5515     275 
   Size:         53      83     186     936    2965     382     635    1451

  Fragments arranged by size: 

                2965    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   5124    5522    5796    5943    6354    5124 
   Size:        398     274     147     411    5461

  Fragments arranged by size: 

                5461     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2231    2261    1178 
   Size:         30     165      30     828      30    5608

  Fragments arranged by size: 

                5608     828     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3558    1186 
   Size:       2372    4319
 
BseYI C'CCAG_C

Cuts at:   5894    6390    5894 
   Size:        496    6195
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    6118
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4141    4902    5930    1540 
   Size:        275     186    2140     761    1028    2301

  Fragments arranged by size: 

                2301    2140    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2518    2546    3069    4752    4837    5908    2518 
   Size:         28     523    1683      85    1071    3301

  Fragments arranged by size: 

                3301    1683    1071     523      85      28
 
BsmI GAATG_Cn'

Cuts at:   4202    4295    4202 
   Size:         93    6598
 
BsmBI CGTCTCn'nnnn_

Cuts at:   3645    3645 
   Size:       6691
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2022    2066    2518    2546    2740    3069    4010 
   Size:        399     232      44     452      28     194     329     941

Cuts at:   4010    4752    4837    5908    1391 
   Size:        742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     941     742     452     399     329     232
                 194      85      44      28
 
BspEI T'CCGG_A

Cuts at:   6354    6354 
   Size:       6691
 
BspHI T'CATG_A

Cuts at:   4580    6310    4580 
   Size:       1730    4961
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    4046     844 
   Size:       3202    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2306    4578    1394 
   Size:        429     483    2272    3507

  Fragments arranged by size: 

                3507    2272     483     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2967    3060    5186    5360      65 
   Size:       1183    1719      93    2126     174    1396

  Fragments arranged by size: 

                2126    1719    1396    1183     174      93
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5335    5522    1700 
   Size:        110    3525     187    2869

  Fragments arranged by size: 

                3525    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6691
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6691
 
BssSI C'ACGA_G

Cuts at:   4751    5763    4751 
   Size:       1012    5679
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2225     794 
   Size:       1431    5260
 
BstBI TT'CG_AA

Cuts at:   5495    5495 
   Size:       6691
 
BstEII G'GTnAC_C

Cuts at:   1184    2315    1184 
   Size:       1131    5560
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3284    3284 
   Size:       6691
 
BstYI r'GATC_y

Cuts at:   1972    2327    3010    3721    3839    4021    4388    4773    4790 
   Size:        355     683     711     118     182     367     385      17

Cuts at:   4790    6242    6328    6340    6686    1972 
   Size:       1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1452     711     683     385     367     355     346
                 182     118      86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2787    3462     513 
   Size:        552    1722     675    3742

  Fragments arranged by size: 

                3742    1722     675     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5952

  Fragments arranged by size: 

                5952     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2904    3216    4207    5001    5021    2904 
   Size:        312     991     794      20    4574

  Fragments arranged by size: 

                4574     991     794     312      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6691
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6656
 
DraI TTT'AAA

Cuts at:   3995    3995 
   Size:       6691
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2405    5698     809 
   Size:        507    1089    3293    1802

  Fragments arranged by size: 

                3293    1802    1089     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2760    4138    5027 
   Size:         54    1425      50     275      18     930    1378     889

Cuts at:   5027    6656       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425    1378     930     889     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    4138    1537 
   Size:        275    2326    4090

  Fragments arranged by size: 

                4090    2326     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3161    3766    3894    4621    1353 
   Size:       1808     605     128     727    3423

  Fragments arranged by size: 

                3423    1808     727     605     128
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2101    5278    5652    5798     387 
   Size:       1064     138     512    3177     374     146    1280

  Fragments arranged by size: 

                3177    1280    1064     512     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2544    2544 
   Size:       6691
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2213    2910    4754    5351    6138    6406    1239 
   Size:        393     581     697    1844     597     787     268    1524

  Fragments arranged by size: 

                1844    1524     787     697     597     581     393     268
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2019    2063    2019 
   Size:         44    6647
 
EcoRI G'AATT_C

Cuts at:   4000    4378    4000 
   Size:        378    6313
 
FspI TGC'GCA

Cuts at:   5197    5197 
   Size:       6691
 
HaeII r_GCGC'y

Cuts at:   1678    5838    1678 
   Size:       4160    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2114    2146    2345    2377    2621 
   Size:         32     135      32     542      32     199      32     244

Cuts at:   2621    2653    3713    3745    5332    5364    5406    5438    1373 
   Size:         32    1060      32    1587      32      42      32    2626

  Fragments arranged by size: 

                2626    1587    1060     542     244     199     135      42
                  32      32      32      32      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    4035    4281     669 
   Size:        988     281    2097     246    3079

  Fragments arranged by size: 

                3079    2097     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2139     748 
   Size:       1391    5300
 
HpaI GTT'AAC

Cuts at:   4281    4281 
   Size:       6691
 
KpnI G_GTAC'C

Cuts at:   4016    4016 
   Size:       6691
 
MfeI C'AATT_G

Cuts at:   4290    4290 
   Size:       6691
 
MluI A'CGCG_T

Cuts at:   6567    6567 
   Size:       6691
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5805    5989    1305 
   Size:       4500     184    2007

  Fragments arranged by size: 

                4500    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2762      10 
   Size:         54    1425     343     930    3939

  Fragments arranged by size: 

                3939    1425     930     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2313    3072    4649    5008    5167     518 
   Size:        648    1147     759    1577     359     159    2042

  Fragments arranged by size: 

                2042    1577    1147     759     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    4107    4784    5932    6177    1606 
   Size:       2501     677    1148     245    2120

  Fragments arranged by size: 

                2501    2120    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2787    3462     513 
   Size:        552    1722     675    3742

  Fragments arranged by size: 

                3742    1722     675     552
 
NdeI CA'TA_TG

Cuts at:    387    3748     387 
   Size:       3361    3330
 
NheI G'CTAG_C

Cuts at:   1052    3904    1052 
   Size:       2852    3839
 
NotI GC'GGCC_GC

Cuts at:   4138    4138 
   Size:       6691
 
NruI TCG'CGA

Cuts at:   2506    2506 
   Size:       6691
 
NspI r_CATG'y

Cuts at:   1132    1229    4049    5594    1132 
   Size:         97    2820    1545    2229

  Fragments arranged by size: 

                2820    2229    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    3617    1348 
   Size:       2269    4422
 
PciI A'CATG_T

Cuts at:   5590    5590 
   Size:       6691
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6691
 
PmeI GTTT'AAAC

Cuts at:   3995    3995 
   Size:       6691
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4760    4792    6273    6305    4760 
   Size:         32    1481      32    5146

  Fragments arranged by size: 

                5146    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6691
 
PsiI TTA'TAA

Cuts at:   3510    4261    3510 
   Size:        751    5940
 
PspOMI G'GGCC_C

Cuts at:   2018    2062    2018 
   Size:         44    6647
 
PstI C_TGCA'G

Cuts at:    830    2203    3404    4043     830 
   Size:       1373    1201     639    3478

  Fragments arranged by size: 

                3478    1373    1201     639
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6691
 
SacI G_AGCT'C

Cuts at:   2546    2546 
   Size:       6691
 
SalI G'TCGA_C

Cuts at:   1936    4033    1936 
   Size:       2097    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   3894    3894 
   Size:       6691
 
SbfI CC_TGCA'GG

Cuts at:   4043    4043 
   Size:       6691
 
ScaI AGT'ACT

Cuts at:   3329    4939    6651    3329 
   Size:       1610    1712    3369

  Fragments arranged by size: 

                3369    1712    1610
 
SfcI C'TryA_G

Cuts at:    826    1045    2199    2586    3400    4039    5174    5855    6046 
   Size:        219    1154     387     814     639    1135     681     191

Cuts at:   6046     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1154    1135     814     681     639     387     219
                 191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6691
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6691
 
SmaI CCC'GGG

Cuts at:   1872    2061    4018    1872 
   Size:        189    1957    4545

  Fragments arranged by size: 

                4545    1957     189
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2276    2349    4795    5696    5958     820 
   Size:        197     926     333      73    2446     901     262    1553

  Fragments arranged by size: 

                2446    1553     926     901     333     262     197      73
            
 
SnaBI TAC'GTA

Cuts at:    493    4482     493 
   Size:       3989    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6691
 
SphI G_CATG'C

Cuts at:   1132    4049    1132 
   Size:       2917    3774
 
SspI AAT'ATT

Cuts at:      5      52    3515    4615       5 
   Size:         47    3463    1100    2081

  Fragments arranged by size: 

                3463    2081    1100      47
 
StuI AGG'CCT

Cuts at:   1517    3452    1517 
   Size:       1935    4756
 
StyI C'CwwG_G

Cuts at:    513    1065    2787    3462    4130     513 
   Size:        552    1722     675     668    3074

  Fragments arranged by size: 

                3074    1722     675     668     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3630    3859    4718    4735    4888    5073    5518 
   Size:       1255    2050     229     859      17     153     185     445

Cuts at:   5518     325 
   Size:       1498

  Fragments arranged by size: 

                2050    1498    1255     859     445     229     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3189    3327    4937 
   Size:        274      80      33      51    1443    1212     138    1610

Cuts at:   4937    6649      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1610    1443    1212     274     138     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2035    2067    2768    2800    6651    6683    2035 
   Size:         32     701      32    3851      32    2043

  Fragments arranged by size: 

                3851    2043     701      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2208    1316 
   Size:        892    5799
 
XbaI T'CTAG_A

Cuts at:   4027    4027 
   Size:       6691
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6691
 
XmaI C'CCGG_G

Cuts at:   1870    2059    4016    1870 
   Size:        189    1957    4545

  Fragments arranged by size: 

                4545    1957     189
 
XmnI GAAnn'nnTTC

Cuts at:   2484    4820    2484 
   Size:       2336    4355
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4499     276 
   Size:         53      83     186     936    2965    2468

  Fragments arranged by size: 

                2965    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NruI
     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI
   PspOMI     PstI     PvuI     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI   Bpu10I    BsiWI  BstZ17I   Bsu36I   DraIII    EcoNI    EcoRV
     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NsiI
     PacI     PfoI     PmlI    PpuMI    PspXI     PsrI    PvuII    RsrII
    SacII    SanDI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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