(Circular) (Six-base) MAPSORT of: pFN21AE2233.seq Check: 1998 from: 1 to: 6691 pFN21AE2233 6691 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4501 278 Size: 53 83 186 936 2965 2468 Fragments arranged by size: 2965 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4034 1937 Size: 2097 4594 Acc65I G'GTAC_C Cuts at: 4012 4012 Size: 6691 AclI AA'CG_TT Cuts at: 1563 4818 5191 1563 Size: 3255 373 3063 Fragments arranged by size: 3255 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4754 6138 6406 1632 Size: 3122 1384 268 1917 Fragments arranged by size: 3122 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2276 820 Size: 197 1259 5235 Fragments arranged by size: 5235 1259 197 AflIII A'CryG_T Cuts at: 1203 3567 5590 6567 1203 Size: 2364 2023 977 1327 Fragments arranged by size: 2364 2023 1327 977 AgeI A'CCGG_T Cuts at: 5522 5522 Size: 6691 AhdI GACnn_n'nnGTC Cuts at: 5420 5420 Size: 6691 AleI CACnn'nnGTG Cuts at: 1166 3072 1166 Size: 1906 4785 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6691 AlwNI CAG_nnn'CTG Cuts at: 2933 4434 6006 2933 Size: 1501 1572 3618 Fragments arranged by size: 3618 1572 1501 ApaI G_GGCC'C Cuts at: 2022 2066 2022 Size: 44 6647 ApaLI G'TGCA_C Cuts at: 4748 5904 4748 Size: 1156 5535 ApoI r'AATT_y Cuts at: 1493 2462 3551 3682 4000 4226 4378 6487 1493 Size: 969 1089 131 318 226 152 2109 1697 Fragments arranged by size: 2109 1697 1089 969 318 226 152 131 AseI AT'TA_AT Cuts at: 160 1032 5245 160 Size: 872 4213 1606 Fragments arranged by size: 4213 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6691 AvaI C'yCGr_G Cuts at: 1870 1943 2059 4016 1870 Size: 73 116 1957 4545 Fragments arranged by size: 4545 1957 116 73 BamHI G'GATC_C Cuts at: 3010 3721 3839 4021 4388 3010 Size: 711 118 182 367 5313 Fragments arranged by size: 5313 711 367 182 118 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4012 5467 618 Size: 325 217 627 2225 1455 1842 Fragments arranged by size: 2225 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2022 2066 2546 4010 1391 Size: 631 44 480 1464 4072 Fragments arranged by size: 4072 1464 631 480 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2120 928 Size: 971 221 5499 Fragments arranged by size: 5499 971 221 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4882 4916 1903 Size: 34 2945 34 3678 Fragments arranged by size: 3678 2945 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4583 5793 4583 Size: 1210 5481 BclI T'GATC_A Cuts at: 1548 3961 1548 Size: 2413 4278 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5302 136 Size: 107 122 71 631 605 3630 1525 Fragments arranged by size: 3630 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2327 6686 2327 Size: 4359 2332 BlpI GC'TnA_GC Cuts at: 2553 2638 4108 2553 Size: 85 1470 5136 Fragments arranged by size: 5136 1470 85 Bme1580I G_kGCm'C Cuts at: 1790 2022 2066 2740 4752 5908 1790 Size: 232 44 674 2012 1156 2573 Fragments arranged by size: 2573 2012 1156 674 232 44 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6691 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4422 5380 442 Size: 416 53 83 399 24 3005 958 1753 Fragments arranged by size: 3005 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3908 1056 Size: 2852 3839 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2134 2578 2134 Size: 444 6247 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 2910 5351 1239 Size: 974 697 2441 2579 Fragments arranged by size: 2579 2441 974 697 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2334 4816 5681 5979 2334 Size: 2482 865 298 3046 Fragments arranged by size: 3046 2482 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2348 5354 882 Size: 617 849 3006 2219 Fragments arranged by size: 3006 2219 849 617 BsaAI yAC'GTr Cuts at: 493 1204 4482 493 Size: 711 3278 2702 Fragments arranged by size: 3278 2702 711 BsaBI GATnn'nnATC Cuts at: 6685 6685 Size: 6691 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4498 4880 5515 275 Size: 53 83 186 936 2965 382 635 1451 Fragments arranged by size: 2965 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5124 5522 5796 5943 6354 5124 Size: 398 274 147 411 5461 Fragments arranged by size: 5461 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2231 2261 1178 Size: 30 165 30 828 30 5608 Fragments arranged by size: 5608 828 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3558 1186 Size: 2372 4319 BseYI C'CCAG_C Cuts at: 5894 6390 5894 Size: 496 6195 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6118 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4141 4902 5930 1540 Size: 275 186 2140 761 1028 2301 Fragments arranged by size: 2301 2140 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2518 2546 3069 4752 4837 5908 2518 Size: 28 523 1683 85 1071 3301 Fragments arranged by size: 3301 1683 1071 523 85 28 BsmI GAATG_Cn' Cuts at: 4202 4295 4202 Size: 93 6598 BsmBI CGTCTCn'nnnn_ Cuts at: 3645 3645 Size: 6691 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2066 2518 2546 2740 3069 4010 Size: 399 232 44 452 28 194 329 941 Cuts at: 4010 4752 4837 5908 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 941 742 452 399 329 232 194 85 44 28 BspEI T'CCGG_A Cuts at: 6354 6354 Size: 6691 BspHI T'CATG_A Cuts at: 4580 6310 4580 Size: 1730 4961 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4046 844 Size: 3202 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2306 4578 1394 Size: 429 483 2272 3507 Fragments arranged by size: 3507 2272 483 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2967 3060 5186 5360 65 Size: 1183 1719 93 2126 174 1396 Fragments arranged by size: 2126 1719 1396 1183 174 93 BsrFI r'CCGG_y Cuts at: 1700 1810 5335 5522 1700 Size: 110 3525 187 2869 Fragments arranged by size: 3525 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6691 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6691 BssSI C'ACGA_G Cuts at: 4751 5763 4751 Size: 1012 5679 BstAPI GCAn_nnn'nTGC Cuts at: 794 2225 794 Size: 1431 5260 BstBI TT'CG_AA Cuts at: 5495 5495 Size: 6691 BstEII G'GTnAC_C Cuts at: 1184 2315 1184 Size: 1131 5560 BstXI CCAn_nnnn'nTGG Cuts at: 3284 3284 Size: 6691 BstYI r'GATC_y Cuts at: 1972 2327 3010 3721 3839 4021 4388 4773 4790 Size: 355 683 711 118 182 367 385 17 Cuts at: 4790 6242 6328 6340 6686 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 711 683 385 367 355 346 182 118 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2787 3462 513 Size: 552 1722 675 3742 Fragments arranged by size: 3742 1722 675 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5952 Fragments arranged by size: 5952 683 56 BtsI GCAGTG_nn' Cuts at: 2904 3216 4207 5001 5021 2904 Size: 312 991 794 20 4574 Fragments arranged by size: 4574 991 794 312 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6691 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6656 DraI TTT'AAA Cuts at: 3995 3995 Size: 6691 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2405 5698 809 Size: 507 1089 3293 1802 Fragments arranged by size: 3293 1802 1089 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2760 4138 5027 Size: 54 1425 50 275 18 930 1378 889 Cuts at: 5027 6656 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1378 930 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4138 1537 Size: 275 2326 4090 Fragments arranged by size: 4090 2326 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3161 3766 3894 4621 1353 Size: 1808 605 128 727 3423 Fragments arranged by size: 3423 1808 727 605 128 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2101 5278 5652 5798 387 Size: 1064 138 512 3177 374 146 1280 Fragments arranged by size: 3177 1280 1064 512 374 146 138 EcoICRI GAG'CTC Cuts at: 2544 2544 Size: 6691 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2910 4754 5351 6138 6406 1239 Size: 393 581 697 1844 597 787 268 1524 Fragments arranged by size: 1844 1524 787 697 597 581 393 268 EcoO109I rG'GnC_Cy Cuts at: 2019 2063 2019 Size: 44 6647 EcoRI G'AATT_C Cuts at: 4000 4378 4000 Size: 378 6313 FspI TGC'GCA Cuts at: 5197 5197 Size: 6691 HaeII r_GCGC'y Cuts at: 1678 5838 1678 Size: 4160 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2114 2146 2345 2377 2621 Size: 32 135 32 542 32 199 32 244 Cuts at: 2621 2653 3713 3745 5332 5364 5406 5438 1373 Size: 32 1060 32 1587 32 42 32 2626 Fragments arranged by size: 2626 1587 1060 542 244 199 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4035 4281 669 Size: 988 281 2097 246 3079 Fragments arranged by size: 3079 2097 988 281 246 HindIII A'AGCT_T Cuts at: 748 2139 748 Size: 1391 5300 HpaI GTT'AAC Cuts at: 4281 4281 Size: 6691 KpnI G_GTAC'C Cuts at: 4016 4016 Size: 6691 MfeI C'AATT_G Cuts at: 4290 4290 Size: 6691 MluI A'CGCG_T Cuts at: 6567 6567 Size: 6691 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5805 5989 1305 Size: 4500 184 2007 Fragments arranged by size: 4500 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2762 10 Size: 54 1425 343 930 3939 Fragments arranged by size: 3939 1425 930 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2313 3072 4649 5008 5167 518 Size: 648 1147 759 1577 359 159 2042 Fragments arranged by size: 2042 1577 1147 759 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4107 4784 5932 6177 1606 Size: 2501 677 1148 245 2120 Fragments arranged by size: 2501 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2787 3462 513 Size: 552 1722 675 3742 Fragments arranged by size: 3742 1722 675 552 NdeI CA'TA_TG Cuts at: 387 3748 387 Size: 3361 3330 NheI G'CTAG_C Cuts at: 1052 3904 1052 Size: 2852 3839 NotI GC'GGCC_GC Cuts at: 4138 4138 Size: 6691 NruI TCG'CGA Cuts at: 2506 2506 Size: 6691 NspI r_CATG'y Cuts at: 1132 1229 4049 5594 1132 Size: 97 2820 1545 2229 Fragments arranged by size: 2820 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3617 1348 Size: 2269 4422 PciI A'CATG_T Cuts at: 5590 5590 Size: 6691 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6691 PmeI GTTT'AAAC Cuts at: 3995 3995 Size: 6691 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4760 4792 6273 6305 4760 Size: 32 1481 32 5146 Fragments arranged by size: 5146 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6691 PsiI TTA'TAA Cuts at: 3510 4261 3510 Size: 751 5940 PspOMI G'GGCC_C Cuts at: 2018 2062 2018 Size: 44 6647 PstI C_TGCA'G Cuts at: 830 2203 3404 4043 830 Size: 1373 1201 639 3478 Fragments arranged by size: 3478 1373 1201 639 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6691 SacI G_AGCT'C Cuts at: 2546 2546 Size: 6691 SalI G'TCGA_C Cuts at: 1936 4033 1936 Size: 2097 4594 SapI GCTCTTCn'nnn_ Cuts at: 3894 3894 Size: 6691 SbfI CC_TGCA'GG Cuts at: 4043 4043 Size: 6691 ScaI AGT'ACT Cuts at: 3329 4939 6651 3329 Size: 1610 1712 3369 Fragments arranged by size: 3369 1712 1610 SfcI C'TryA_G Cuts at: 826 1045 2199 2586 3400 4039 5174 5855 6046 Size: 219 1154 387 814 639 1135 681 191 Cuts at: 6046 826 Size: 1471 Fragments arranged by size: 1471 1154 1135 814 681 639 387 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6691 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6691 SmaI CCC'GGG Cuts at: 1872 2061 4018 1872 Size: 189 1957 4545 Fragments arranged by size: 4545 1957 189 SmlI C'TyrA_G Cuts at: 820 1017 1943 2276 2349 4795 5696 5958 820 Size: 197 926 333 73 2446 901 262 1553 Fragments arranged by size: 2446 1553 926 901 333 262 197 73 SnaBI TAC'GTA Cuts at: 493 4482 493 Size: 3989 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6691 SphI G_CATG'C Cuts at: 1132 4049 1132 Size: 2917 3774 SspI AAT'ATT Cuts at: 5 52 3515 4615 5 Size: 47 3463 1100 2081 Fragments arranged by size: 3463 2081 1100 47 StuI AGG'CCT Cuts at: 1517 3452 1517 Size: 1935 4756 StyI C'CwwG_G Cuts at: 513 1065 2787 3462 4130 513 Size: 552 1722 675 668 3074 Fragments arranged by size: 3074 1722 675 668 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3630 3859 4718 4735 4888 5073 5518 Size: 1255 2050 229 859 17 153 185 445 Cuts at: 5518 325 Size: 1498 Fragments arranged by size: 2050 1498 1255 859 445 229 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3189 3327 4937 Size: 274 80 33 51 1443 1212 138 1610 Cuts at: 4937 6649 96 Size: 1712 138 Fragments arranged by size: 1712 1610 1443 1212 274 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2035 2067 2768 2800 6651 6683 2035 Size: 32 701 32 3851 32 2043 Fragments arranged by size: 3851 2043 701 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2208 1316 Size: 892 5799 XbaI T'CTAG_A Cuts at: 4027 4027 Size: 6691 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6691 XmaI C'CCGG_G Cuts at: 1870 2059 4016 1870 Size: 189 1957 4545 Fragments arranged by size: 4545 1957 189 XmnI GAAnn'nnTTC Cuts at: 2484 4820 2484 Size: 2336 4355 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4499 276 Size: 53 83 186 936 2965 2468 Fragments arranged by size: 2965 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI