Restriction map for
pFN21AE2233
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BciVI
BglI BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FseI FspI HaeII Hin1I HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MspA1I
MunI NaeI NarI NgoMIV NotI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AflII 271 CTTAAG C^TTAAG
AflIII 1562 ACGTGT A^C[GA][CT]GT
AlwNI 923 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 475 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 200 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 54 CCCGGG C^[CT]CG[GA]G
AvaII 199 GGACC G^G[AT]CC
BalI 755 TGGCCA TGG^CCA
BbsI 108 GAAGAC GAAGAC(2/6)
BclI 1956 TGATCA T^GATCA
BfaI 1900 CTAG C^TAG
BfrI 271 CTTAAG C^TTAAG
BglII 322 AGATCT A^GATCT
BpuAI 108 GAAGAC GAAGAC(2/6)
BsaI 349 GAGACC GGTCTC(1/5)
BseRI 1538 GAGGAG GAGGAG(10/8)
BsmBI 1634 CGTCTC CGTCTC(1/5)
BsoBI 54 CCCGGG C^[CT]CG[GA]G
BsrBI 299 CCGCTC CCGCTC(-3/-3)
BstAPI 214 GCAATCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 310 GGTGACC G^GT[ACGT]ACC
BstPI 310 GGTGACC G^GT[ACGT]ACC
BstXI 1272 CCAATATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 24 GCGC GCG^C
DrdI 394 GACACTATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 755 TGGCCA [CT]^GGCC[GA]
EcoO65I 310 GGTGACC G^GT[ACGT]ACC
FbaI 1956 TGATCA T^GATCA
HhaI 24 GCGC GCG^C
HinP1I 24 GCGC G^CGC
HindIII 134 AAGCTT A^AGCTT
MaeI 1900 CTAG C^TAG
MluNI 755 TGGCCA TGG^CCA
MscI 755 TGGCCA TGG^CCA
NdeI 1742 CATATG CA^TATG
NheI 1899 GCTAGC G^CTAGC
NruI 499 TCGCGA TCG^CGA
PflFI 200 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 537 GAGCTC GAGCT^C
SapI 1894 GAAGAGC GCTCTTC(1/4)
ScaI 1322 AGTACT AGT^ACT
SmaI 54 CCCGGG CCC^GGG
SspI 1508 AATATT AAT^ATT
StuI 1445 AGGCCT AGG^CCT
TaqI 1536 TCGA T^CGA
Tth111I 200 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1536 TCGA T^CGA
XmaCI 54 CCCGGG C^CCGGG
XmaI 54 CCCGGG C^CCGGG
XmnI 475 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 23 CGCG CG^CG
500 CGCG
ApaI 13 GGGCCC GGGCC^C
57 GGGCCC
BlpI 547 GCTGAGC GC^T[ACGT]AGC
632 GCTCAGC
BpmI 920 CTCCAG CTGGAG(16/14)
187 CTGGAG
Bpu1102I 547 GCTGAGC GC^T[ACGT]AGC
632 GCTCAGC
BsrDI 955 GCAATG GCAATG(2/0)
1048 GCAATG
BstUI 23 CGCG CG^CG
500 CGCG
CelII 547 GCTGAGC GC^T[ACGT]AGC
632 GCTCAGC
DraII 13 GGGCCCT [GA]G^G[ACGT]CC[CT]
57 GGGCCCC
DsaI 782 CCATGG C^C[GA][CT]GG
1457 CCATGG
EcoO109I 13 GGGCCCT [GA]G^G[ACGT]CC[CT]
57 GGGCCCC
EcoT14I 782 CCATGG C^C[AT][AT]GG
1457 CCATGG
HgaI 586 GCGTC GACGC(5/10)
1633 GCGTC
MaeII 1440 ACGT A^CGT
1562 ACGT
MslI 304 CATTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1063 CACTTTAGTG
MvnI 23 CGCG CG^CG
500 CGCG
NcoI 782 CCATGG C^CATGG
1457 CCATGG
PstI 194 CTGCAG CTGCA^G
1395 CTGCAG
SmlI 271 CTTAAG C^T[CT][GA]AG
344 CTCAAG
StyI 782 CCATGG C^C[AT][AT]GG
1457 CCATGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BamHI BanII BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII