KLHL15 ORF clone FHC00264

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00264

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Clone info.

Species

human

Clone Name

pFN21AA1677 Sequence (with vector) Restriction Map

Product ID

FHC00264

Flexi Clone PID

FXC00264

Original Clone PID

ORK00264

ORF Sequence

DNA sequence (1812 bp)
Protein sequence (604 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384623]

Gene

KIAA1677

Symbol

KLHL15

Alias : HEL-S-305, XLID103

Description

kelch-like family member 15

Original Clone ID

fg04234

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1812 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 604 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS35217.1 0 100.0 KLHL15 CCDS55479.1 2.7e-50 32.2 KLHL13 CCDS55481.1 2.8e-50 32.2 KLHL13 CCDS14571.1 2.9e-50 32.2 KLHL13 CCDS55480.1 2.9e-50 32.2 KLHL13 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013069 22 126 PF00651 BTB/POZ IPR011705 146 236 PF07707 BTB/Kelch-associated IPR006652 344 366 PF01344 Kelch repeat type 1 IPR006652 368 409 PF01344 Kelch repeat type 1 IPR006652 477 529 PF01344 Kelch repeat type 1 IPR006652 532 572 PF01344 Kelch repeat type 1 HMMSmart IPR000210 31 128 SM00225 BTB/POZ-like IPR011705 133 237 SM00875 BTB/Kelch-associated IPR006652 328 379 SM00612 Kelch repeat type 1 IPR006652 380 426 SM00612 Kelch repeat type 1 IPR006652 427 488 SM00612 Kelch repeat type 1 IPR006652 489 542 SM00612 Kelch repeat type 1 IPR006652 543 592 SM00612 Kelch repeat type 1 ProfileScan IPR000210 31 98 PS50097 BTB/POZ-like
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 108 AMYVQLIEVVKFCCSFLLAKICL 130 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_006724581 604 300980,300982 100.0 100.0 NP_085127 604 300980,300982 100.0 100.0 NP_001161775 613 300655 32.2 98.0 NP_001161773 639 300655 32.2 98.0 NP_001161774 639 300655 32.2 98.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA1677 Download

>KIAA1677  1812 bp
ATGGCAGGGGACGTGGAAGGATTCTGTTCCTCCATCCACGACACCAGTGTCTCTGCTGGG
TTCAGAGCACTGTATGAGGAGGGATTGCTTCTTGATGTCACTCTGGTTATTGAAGATCAT
CAGTTCCAGGCCCATAAAGCACTCTTGGCCACCCAGAGTGATTACTTCAGAATTATGTTT
ACTGCAGACATGAGGGAACGAGATCAGGACAAAATTCATTTAAAAGGTCTAACAGCTACC
GGTTTCAGCCATGTCCTGCAATTTATGTACTATGGAACTATAGAGCTGAGTATGAATACC
GTTCATGAGATTCTTCAGGCTGCCATGTATGTTCAACTTATAGAAGTGGTGAAGTTCTGC
TGCTCTTTTCTCTTAGCGAAGATCTGCCTAGAAAATTGTGCAGAAATTATGAGACTCTTA
GATGATTTCGGCGTAAACATCGAGGGAGTCAGGGAGAAGTTAGACACCTTTCTGCTAGAC
AACTTTGTGCCACTCATGTCTAGGCCTGACTTTCTGTCCTATTTGAGCTTTGAGAAGCTC
ATGTCTTACTTGGATAATGATCATCTGAGCAGGTTCCCAGAGATAGAGCTGTACGAGGCT
GTGCAGTCTTGGCTGCGGCATGATAGAAGACGCTGGAGACATACCGATACCATCATTCAG
AATATCCGGTTTTGCTTGATGACCCCAACCAGCGTTTTTGAGAAGGTTAAGACATCAGAA
TTTTATAGATACTCCCGACAGCTCCGTTACGAAGTTGACCAAGCATTGAATTACTTTCAG
AATGTTCACCAGCAGCCTTTGTTGGATATGAAGTCAAGCCGCATCCGTTCTGCAAAACCG
CAAACTACAGTATTTCGAGGAATGATTGGACATAGCATGGTTAACAGTAAAATACTTCTC
TTAAAGAAACCAAGAGTCTGGTGGGAGCTAGAAGGCCCACAAGTACCTCTGCGACCTGAC
TGCCTTGCTATCGTCAATAATTTTGTGTTCCTGTTAGGCGGGGAAGAGCTGGGCCCGGAT
GGTGAATTCCATGCTTCTTCCAAAGTATTCAGGTATGACCCGAGACAGAACTCCTGGCTG
CAGATGGCAGATATGTCTGTACCACGCTCTGAATTTGCTGTAGGTGTTATTGGGAAGTTT
ATTTACGCCGTAGCAGGCAGAACCAGAGATGAGACTTTCTATTCAACTGAGAGATATGAC
ATCACCAACGATAAATGGGAATTTGTGGATCCTTATCCAGTTAACAAATATGGACATGAG
GGGACAGTGCTCAATAACAAATTGTTTATCACCGGTGGAATCACCTCATCTTCCACCTCC
AAGCAAGTGTGCGTGTTTGACCCCAGCAAAGAAGGGACCATAGAACAACGGACCAGGAGA
ACTCAAGTGGTTACCAACTGTTGGGAGAATAAGAGCAAGATGAATTACGCGAGATGCTTT
CACAAGATGATTTCTTACAATGGCAAGCTTTATGTCTTCGGTGGTGTCTGTGTGATCTTG
AGGGCCTCTTTCGAATCTCAGGGATGCCCTTCTACAGAAGTATACAACCCAGAGACTGAT
CAGTGGACCATCTTGGCATCCATGCCGATTGGTAGAAGTGGCCATGGTGTGACTGTGCTG
GACAAACAAATAATGGTTCTTGGAGGCCTTTGTTATAATGGTCATTACAGCGATTCCATC
CTCACTTTTGATCCGGATGAAAACAAGTGGAAGGAAGATGAGTACCCTCGGATGCCCTGC
AAGCTGGATGGTTTACAAGTATGCAACCTGCATTTTCCGGACTATGTACTGGATGAGGTC
AGGCGTTGCAAC

ORF Protein

Cloned ORF protein sequence for pFN21AA1677 Download

>KIAA1677  604 aa
MAGDVEGFCSSIHDTSVSAGFRALYEEGLLLDVTLVIEDHQFQAHKALLATQSDYFRIMF
TADMRERDQDKIHLKGLTATGFSHVLQFMYYGTIELSMNTVHEILQAAMYVQLIEVVKFC
CSFLLAKICLENCAEIMRLLDDFGVNIEGVREKLDTFLLDNFVPLMSRPDFLSYLSFEKL
MSYLDNDHLSRFPEIELYEAVQSWLRHDRRRWRHTDTIIQNIRFCLMTPTSVFEKVKTSE
FYRYSRQLRYEVDQALNYFQNVHQQPLLDMKSSRIRSAKPQTTVFRGMIGHSMVNSKILL
LKKPRVWWELEGPQVPLRPDCLAIVNNFVFLLGGEELGPDGEFHASSKVFRYDPRQNSWL
QMADMSVPRSEFAVGVIGKFIYAVAGRTRDETFYSTERYDITNDKWEFVDPYPVNKYGHE
GTVLNNKLFITGGITSSSTSKQVCVFDPSKEGTIEQRTRRTQVVTNCWENKSKMNYARCF
HKMISYNGKLYVFGGVCVILRASFESQGCPSTEVYNPETDQWTILASMPIGRSGHGVTVL
DKQIMVLGGLCYNGHYSDSILTFDPDENKWKEDEYPRMPCKLDGLQVCNLHFPDYVLDEV
RRCN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA1677 Download

>pFN21AA1677  6517 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAGGGGACGTGGAAGGATTCTGTTCCTCCAT
CCACGACACCAGTGTCTCTGCTGGGTTCAGAGCACTGTATGAGGAGGGATTGCTTCTTGA
TGTCACTCTGGTTATTGAAGATCATCAGTTCCAGGCCCATAAAGCACTCTTGGCCACCCA
GAGTGATTACTTCAGAATTATGTTTACTGCAGACATGAGGGAACGAGATCAGGACAAAAT
TCATTTAAAAGGTCTAACAGCTACCGGTTTCAGCCATGTCCTGCAATTTATGTACTATGG
AACTATAGAGCTGAGTATGAATACCGTTCATGAGATTCTTCAGGCTGCCATGTATGTTCA
ACTTATAGAAGTGGTGAAGTTCTGCTGCTCTTTTCTCTTAGCGAAGATCTGCCTAGAAAA
TTGTGCAGAAATTATGAGACTCTTAGATGATTTCGGCGTAAACATCGAGGGAGTCAGGGA
GAAGTTAGACACCTTTCTGCTAGACAACTTTGTGCCACTCATGTCTAGGCCTGACTTTCT
GTCCTATTTGAGCTTTGAGAAGCTCATGTCTTACTTGGATAATGATCATCTGAGCAGGTT
CCCAGAGATAGAGCTGTACGAGGCTGTGCAGTCTTGGCTGCGGCATGATAGAAGACGCTG
GAGACATACCGATACCATCATTCAGAATATCCGGTTTTGCTTGATGACCCCAACCAGCGT
TTTTGAGAAGGTTAAGACATCAGAATTTTATAGATACTCCCGACAGCTCCGTTACGAAGT
TGACCAAGCATTGAATTACTTTCAGAATGTTCACCAGCAGCCTTTGTTGGATATGAAGTC
AAGCCGCATCCGTTCTGCAAAACCGCAAACTACAGTATTTCGAGGAATGATTGGACATAG
CATGGTTAACAGTAAAATACTTCTCTTAAAGAAACCAAGAGTCTGGTGGGAGCTAGAAGG
CCCACAAGTACCTCTGCGACCTGACTGCCTTGCTATCGTCAATAATTTTGTGTTCCTGTT
AGGCGGGGAAGAGCTGGGCCCGGATGGTGAATTCCATGCTTCTTCCAAAGTATTCAGGTA
TGACCCGAGACAGAACTCCTGGCTGCAGATGGCAGATATGTCTGTACCACGCTCTGAATT
TGCTGTAGGTGTTATTGGGAAGTTTATTTACGCCGTAGCAGGCAGAACCAGAGATGAGAC
TTTCTATTCAACTGAGAGATATGACATCACCAACGATAAATGGGAATTTGTGGATCCTTA
TCCAGTTAACAAATATGGACATGAGGGGACAGTGCTCAATAACAAATTGTTTATCACCGG
TGGAATCACCTCATCTTCCACCTCCAAGCAAGTGTGCGTGTTTGACCCCAGCAAAGAAGG
GACCATAGAACAACGGACCAGGAGAACTCAAGTGGTTACCAACTGTTGGGAGAATAAGAG
CAAGATGAATTACGCGAGATGCTTTCACAAGATGATTTCTTACAATGGCAAGCTTTATGT
CTTCGGTGGTGTCTGTGTGATCTTGAGGGCCTCTTTCGAATCTCAGGGATGCCCTTCTAC
AGAAGTATACAACCCAGAGACTGATCAGTGGACCATCTTGGCATCCATGCCGATTGGTAG
AAGTGGCCATGGTGTGACTGTGCTGGACAAACAAATAATGGTTCTTGGAGGCCTTTGTTA
TAATGGTCATTACAGCGATTCCATCCTCACTTTTGATCCGGATGAAAACAAGTGGAAGGA
AGATGAGTACCCTCGGATGCCCTGCAAGCTGGATGGTTTACAAGTATGCAACCTGCATTT
TCCGGACTATGTACTGGATGAGGTCAGGCGTTGCAACGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT