Restriction map for
pFN21AA1677
##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BanI BbeI
BbrPI BbvCI BciVI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI BstAPI Bsu36I
CelII CfoI ClaI CpoI DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV EcoT22I FseI FspI
HaeII HhaI Hin1I HinP1I KasI
KpnI KspI MfeI MluI MslI
MspA1I MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 1540 GTATAC GT^[AC][GT]AC
AccII 1428 CGCG CG^CG
AhdI 508 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1550 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1011 GGGCCC GGGCC^C
AspEI 508 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1059 CCCGAG C^[CT]CG[GA]G
BamHI 1227 GGATCC G^GATCC
BanII 1011 GGGCCC G[GA]GC[CT]^C
BcgI 952 CGACCTGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1014 CCCGG CC^[GC]GG
BglII 380 AGATCT A^GATCT
BpmI 633 CTGGAG CTGGAG(16/14)
BseRI 76 GAGGAG GAGGAG(10/8)
BsiYI 1394 CCAACTGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1394 CCAACTGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 1059 CCCGAG C^[CT]CG[GA]G
BspHI 303 TCATGA T^CATGA
Bst1107I 1540 GTATAC GTA^TAC
BstBI 1510 TTCGAA TT^CGAA
BstEII 1389 GGTTACC G^GT[ACGT]ACC
BstPI 1389 GGTTACC G^GT[ACGT]ACC
BstUI 1428 CGCG CG^CG
BstXI 910 CCAAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1540 GTATAC GTA^TAC
DraI 219 TTTAAA TTT^AAA
DraII 1501 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 40 GACACCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1602 CCATGG C^C[GA][CT]GG
Eam1105I 508 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1003 GAAGAG CTCTTC(1/4)
EcoO109I 1501 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1389 GGTTACC G^GT[ACGT]ACC
EcoRI 1024 GAATTC G^AATTC
EcoT14I 1602 CCATGG C^C[AT][AT]GG
FauI 998 GCGGG CCCGC(4/6)
HgaI 629 GACGC GACGC(5/10)
HindIII 1465 AAGCTT A^AGCTT
Ksp632I 1003 GAAGAG CTCTTC(1/4)
MaeII 11 ACGT A^CGT
MvnI 1428 CGCG CG^CG
NciI 1014 CCCGG CC^[GC]GG
NcoI 1602 CCATGG C^CATGG
NspV 1510 TTCGAA TT^CGAA
PflMI 1394 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 303 TCATGA T^CATGA
SapI 1003 GAAGAGC GCTCTTC(1/4)
SfuI 1510 TTCGAA TT^CGAA
SgrAI 1290 CACCGGTG C[GA]^CCGG[CT]G
StyI 1602 CCATGG C^C[AT][AT]GG
Van91I 1394 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 910 CCAAGAGTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 1692 TCCGGA T^CCGGA
1776 TCCGGA
AgeI 238 ACCGGT A^CCGGT
1291 ACCGGT
AspHI 65 GAGCAC G[AT]GC[AT]^C
1267 GTGCTC
BalI 146 TGGCCA TGG^CCA
1599 TGGCCA
BbsI 626 GAAGAC GAAGAC(2/6)
1474 GTCTTC
BclI 558 TGATCA T^GATCA
1557 TGATCA
BpuAI 626 GAAGAC GAAGAC(2/6)
1474 GTCTTC
BsaBI 553 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1228 GATCCTTATC
BsaJI 1602 CCATGG C^C[ACGT][ACGT]GG
1726 CCTCGG
BseAI 1692 TCCGGA T^CCGGA
1776 TCCGGA
BsgI 398 GTGCAG GTGCAG(16/14)
601 GTGCAG
BsiHKAI 65 GAGCAC G[AT]GC[AT]^C
1267 GTGCTC
BspEI 1692 TCCGGA T^CCGGA
1776 TCCGGA
BspMI 1766 ACCTGC ACCTGC(4/8)
569 GCAGGT
BsrFI 238 ACCGGT [GA]^CCGG[CT]
1291 ACCGGT
BstYI 380 AGATCT [GA]^GATC[CT]
1227 GGATCC
Cfr10I 238 ACCGGT [GA]^CCGG[CT]
1291 ACCGGT
EaeI 146 TGGCCA [CT]^GGCC[GA]
1599 TGGCCA
FbaI 558 TGATCA T^GATCA
1557 TGATCA
HpaI 880 GTTAAC GTT^AAC
1240 GTTAAC
MamI 553 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1228 GATCCTTATC
MflI 380 AGATCT [GA]^GATC[CT]
1227 GGATCC
MluNI 146 TGGCCA TGG^CCA
1599 TGGCCA
MroI 1692 TCCGGA T^CCGGA
1776 TCCGGA
MscI 146 TGGCCA TGG^CCA
1599 TGGCCA
PinAI 238 ACCGGT A^CCGGT
1291 ACCGGT
PstI 182 CTGCAG CTGCA^G
1078 CTGCAG
SmlI 1382 CTCAAG C^T[CT][GA]AG
1497 CTTGAG
StuI 502 AGGCCT AGG^CCT
1644 AGGCCT
Tsp45I 97 GTCAC ^GT[GC]AC
1609 GTGAC
XhoII 380 AGATCT [GA]^GATC[CT]
1227 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BfaI BmyI
BsaWI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I