(Circular) (Six-base) MAPSORT of: pFN21AA1677.seq Check: 194 from: 1 to: 6517 pFN21AA1677 6517 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4327 278 Size: 53 83 186 936 2791 2468 Fragments arranged by size: 2791 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3546 3860 1937 Size: 1609 314 4594 Fragments arranged by size: 4594 1609 314 Acc65I G'GTAC_C Cuts at: 3838 3838 Size: 6517 AclI AA'CG_TT Cuts at: 1563 3816 4644 5017 1563 Size: 2253 828 373 3063 Fragments arranged by size: 3063 2253 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2155 2303 4580 5964 6232 1632 Size: 523 148 2277 1384 268 1917 Fragments arranged by size: 2277 1917 1384 523 268 148 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6320 AflIII A'CryG_T Cuts at: 1203 5416 6393 1203 Size: 4213 977 1327 Fragments arranged by size: 4213 1327 977 AgeI A'CCGG_T Cuts at: 2243 3296 5348 2243 Size: 1053 2052 3412 Fragments arranged by size: 3412 2052 1053 AhdI GACnn_n'nnGTC Cuts at: 2518 5246 2518 Size: 2728 3789 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6517 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6517 AlwNI CAG_nnn'CTG Cuts at: 3560 4260 5832 3560 Size: 700 1572 4245 Fragments arranged by size: 4245 1572 700 ApaI G_GGCC'C Cuts at: 3020 3020 Size: 6517 ApaLI G'TGCA_C Cuts at: 4574 5730 4574 Size: 1156 5361 ApoI r'AATT_y Cuts at: 1493 2217 2723 3029 3116 3224 3826 4052 4204 Size: 724 506 306 87 108 602 226 152 Cuts at: 4204 6313 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 724 602 506 306 226 152 108 87 AseI AT'TA_AT Cuts at: 160 1032 5071 160 Size: 872 4039 1606 Fragments arranged by size: 4039 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6517 AvaI C'yCGr_G Cuts at: 1870 1943 3064 3842 1870 Size: 73 1121 778 4545 Fragments arranged by size: 4545 1121 778 73 BamHI G'GATC_C Cuts at: 3232 3847 4214 3232 Size: 615 367 5535 Fragments arranged by size: 5535 615 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3838 5293 618 Size: 325 217 627 2051 1455 1842 Fragments arranged by size: 2051 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3020 3836 1391 Size: 1629 816 4072 Fragments arranged by size: 4072 1629 816 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2638 3472 928 Size: 971 739 834 3973 Fragments arranged by size: 3973 971 834 739 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2946 2980 4708 4742 1903 Size: 34 1009 34 1728 34 3678 Fragments arranged by size: 3678 1728 1009 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4409 5619 4409 Size: 1210 5307 BclI T'GATC_A Cuts at: 1548 2563 3562 1548 Size: 1015 999 4503 Fragments arranged by size: 4503 1015 999 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5128 136 Size: 107 122 71 631 605 3456 1525 Fragments arranged by size: 3456 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2385 6512 2385 Size: 4127 2390 BlpI GC'TnA_GC Cuts at: 3934 3934 Size: 6517 Bme1580I G_kGCm'C Cuts at: 1790 3020 4578 5734 1790 Size: 1230 1558 1156 2573 Fragments arranged by size: 2573 1558 1230 1156 BmgBI CAC'GTC Cuts at: 1318 2017 1318 Size: 699 5818 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4248 5206 442 Size: 416 53 83 399 24 2831 958 1753 Fragments arranged by size: 2831 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6517 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2659 5177 1239 Size: 1420 2518 2579 Fragments arranged by size: 2579 2518 1420 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3372 3523 4642 5507 5805 3372 Size: 151 1119 865 298 4084 Fragments arranged by size: 4084 1119 865 298 151 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5180 882 Size: 617 3681 2219 Fragments arranged by size: 3681 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4308 493 Size: 711 3104 2702 Fragments arranged by size: 3104 2702 711 BsaBI GATnn'nnATC Cuts at: 2562 3237 6511 2562 Size: 675 3274 2568 Fragments arranged by size: 3274 2568 675 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4324 4706 5341 275 Size: 53 83 186 936 2791 382 635 1451 Fragments arranged by size: 2791 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2243 2670 3296 3697 3781 4950 5348 5622 5769 Size: 427 626 401 84 1169 398 274 147 Cuts at: 5769 6180 2243 Size: 411 2580 Fragments arranged by size: 2580 1169 626 427 411 401 398 274 147 84 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6292 Fragments arranged by size: 6292 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 1186 Size: 910 5607 BseYI C'CCAG_C Cuts at: 2060 3013 3347 5720 6216 2060 Size: 953 334 2373 496 2361 Fragments arranged by size: 2373 2361 953 496 334 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2424 2627 1163 Size: 573 688 203 5053 Fragments arranged by size: 5053 688 573 203 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3967 4728 5756 1540 Size: 275 186 1966 761 1028 2301 Fragments arranged by size: 2301 1966 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2074 3276 4578 4663 5734 2074 Size: 1202 1302 85 1071 2857 Fragments arranged by size: 2857 1302 1202 1071 85 BsmI GAATG_Cn' Cuts at: 4028 4121 4028 Size: 93 6424 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2074 3020 3276 3836 4578 4663 5734 Size: 399 284 946 256 560 742 85 1071 Cuts at: 5734 1391 Size: 2174 Fragments arranged by size: 2174 1071 946 742 560 399 284 256 85 BspEI T'CCGG_A Cuts at: 3697 3781 6180 3697 Size: 84 2399 4034 Fragments arranged by size: 4034 2399 84 BspHI T'CATG_A Cuts at: 2308 4406 6136 2308 Size: 2098 1730 2689 Fragments arranged by size: 2689 2098 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2565 3780 3872 844 Size: 1721 1215 92 3489 Fragments arranged by size: 3489 1721 1215 92 BsrBI CCG'CTC Cuts at: 1394 1823 4404 1394 Size: 429 2581 3507 Fragments arranged by size: 3507 2581 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5012 5186 65 Size: 1183 3764 174 1396 Fragments arranged by size: 3764 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2243 3296 5161 5348 1700 Size: 110 433 1053 1865 187 2869 Fragments arranged by size: 2869 1865 1053 433 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6517 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6517 BssSI C'ACGA_G Cuts at: 4577 5589 4577 Size: 1012 5505 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6517 BstBI TT'CG_AA Cuts at: 3516 5321 3516 Size: 1805 4712 BstEII G'GTnAC_C Cuts at: 1184 3394 1184 Size: 2210 4307 BstXI CCAn_nnnn'nTGG Cuts at: 2922 2922 Size: 6517 BstYI r'GATC_y Cuts at: 1972 2385 3232 3847 4214 4599 4616 6068 6154 Size: 413 847 615 367 385 17 1452 86 Cuts at: 6154 6166 6512 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 847 615 413 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3547 3547 Size: 6517 BtgI C'CryG_G Cuts at: 513 1065 2004 3607 513 Size: 552 939 1603 3423 Fragments arranged by size: 3423 1603 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5778 Fragments arranged by size: 5778 683 56 BtsI GCAGTG_nn' Cuts at: 4033 4827 4847 4033 Size: 794 20 5703 Fragments arranged by size: 5703 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6517 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6482 DraI TTT'AAA Cuts at: 2226 3821 2226 Size: 1595 4922 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2051 5524 809 Size: 507 735 3473 1802 Fragments arranged by size: 3473 1802 735 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2151 3604 3964 Size: 54 1425 50 275 18 321 1453 360 Cuts at: 3964 4853 6482 8 Size: 889 1629 43 Fragments arranged by size: 1629 1453 1425 889 360 321 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3964 1537 Size: 275 2152 4090 Fragments arranged by size: 4090 2152 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3003 4447 1353 Size: 1650 1444 3423 Fragments arranged by size: 3423 1650 1444 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5104 5478 5624 387 Size: 1064 138 3515 374 146 1280 Fragments arranged by size: 3515 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2155 2303 2659 4580 5177 5964 6232 Size: 393 523 148 356 1921 597 787 268 Cuts at: 6232 1239 Size: 1524 Fragments arranged by size: 1921 1524 787 597 523 393 356 268 148 EcoO109I rG'GnC_Cy Cuts at: 3507 3507 Size: 6517 EcoRI G'AATT_C Cuts at: 3029 3826 4204 3029 Size: 797 378 5342 Fragments arranged by size: 5342 797 378 FspI TGC'GCA Cuts at: 5023 5023 Size: 6517 HaeII r_GCGC'y Cuts at: 1678 5664 1678 Size: 3986 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2504 2536 2801 2833 3189 Size: 32 135 32 932 32 265 32 356 Cuts at: 3189 3221 5158 5190 5232 5264 1373 Size: 32 1937 32 42 32 2626 Fragments arranged by size: 2626 1937 932 356 265 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2761 2887 3247 3861 4107 669 Size: 988 281 823 126 360 614 246 3079 Fragments arranged by size: 3079 988 823 614 360 281 246 126 HindIII A'AGCT_T Cuts at: 748 3470 748 Size: 2722 3795 HpaI GTT'AAC Cuts at: 2887 3247 4107 2887 Size: 360 860 5297 Fragments arranged by size: 5297 860 360 KpnI G_GTAC'C Cuts at: 3842 3842 Size: 6517 MfeI C'AATT_G Cuts at: 4116 4116 Size: 6517 MluI A'CGCG_T Cuts at: 6393 6393 Size: 6517 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2787 5631 5815 1305 Size: 1482 2844 184 2007 Fragments arranged by size: 2844 2007 1482 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2153 3606 10 Size: 54 1425 343 321 1453 2921 Fragments arranged by size: 2921 1453 1425 343 321 54 MslI CAynn'nnrTG Cuts at: 518 1166 4475 4834 4993 518 Size: 648 3309 359 159 2042 Fragments arranged by size: 3309 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3933 4610 5758 6003 1606 Size: 2327 677 1148 245 2120 Fragments arranged by size: 2327 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3607 513 Size: 552 939 1603 3423 Fragments arranged by size: 3423 1603 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6517 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6517 NotI GC'GGCC_GC Cuts at: 3964 3964 Size: 6517 NspI r_CATG'y Cuts at: 1132 1229 3875 5420 1132 Size: 97 2646 1545 2229 Fragments arranged by size: 2646 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6517 PciI A'CATG_T Cuts at: 5416 5416 Size: 6517 PflMI CCAn_nnn'nTGG Cuts at: 1108 3405 1108 Size: 2297 4220 PmeI GTTT'AAAC Cuts at: 3821 3821 Size: 6517 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4586 4618 6099 6131 4586 Size: 32 1481 32 4972 Fragments arranged by size: 4972 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6517 PsiI TTA'TAA Cuts at: 3660 4087 3660 Size: 427 6090 PspOMI G'GGCC_C Cuts at: 3016 3016 Size: 6517 PstI C_TGCA'G Cuts at: 830 2191 3087 3869 830 Size: 1361 896 782 3478 Fragments arranged by size: 3478 1361 896 782 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6517 SalI G'TCGA_C Cuts at: 1936 3859 1936 Size: 1923 4594 SapI GCTCTTCn'nnn_ Cuts at: 3003 3003 Size: 6517 SbfI CC_TGCA'GG Cuts at: 3869 3869 Size: 6517 ScaI AGT'ACT Cuts at: 4765 6477 4765 Size: 1712 4805 SfcI C'TryA_G Cuts at: 826 1045 2187 2283 2850 3083 3123 3537 3865 Size: 219 1142 96 567 233 40 414 328 Cuts at: 3865 5000 5681 5872 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1142 1135 681 567 414 328 233 219 191 96 40 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6517 SgrAI Cr'CCGG_yG Cuts at: 1700 3296 1700 Size: 1596 4921 SmaI CCC'GGG Cuts at: 1872 3844 1872 Size: 1972 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3387 3502 4621 5522 5784 820 Size: 197 926 1444 115 1119 901 262 1553 Fragments arranged by size: 1553 1444 1119 926 901 262 197 115 SnaBI TAC'GTA Cuts at: 493 4308 493 Size: 3815 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6517 SphI G_CATG'C Cuts at: 1132 3875 1132 Size: 2743 3774 SspI AAT'ATT Cuts at: 5 52 4441 5 Size: 47 4389 2081 Fragments arranged by size: 4389 2081 47 StuI AGG'CCT Cuts at: 1517 2509 3651 1517 Size: 992 1142 4383 Fragments arranged by size: 4383 1142 992 StyI C'CwwG_G Cuts at: 513 1065 2004 3607 3956 513 Size: 552 939 1603 349 3074 Fragments arranged by size: 3074 1603 939 552 349 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2171 4544 4561 4714 4899 5344 325 Size: 1255 591 2373 17 153 185 445 1498 Fragments arranged by size: 2373 1498 1255 591 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2271 3790 4763 Size: 274 80 33 51 1443 294 1519 973 Cuts at: 4763 6475 96 Size: 1712 138 Fragments arranged by size: 1712 1519 1443 973 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3679 3711 6477 6509 3679 Size: 32 2766 32 3687 Fragments arranged by size: 3687 2766 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6517 XbaI T'CTAG_A Cuts at: 3853 3853 Size: 6517 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2922 2922 Size: 6517 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6517 XmaI C'CCGG_G Cuts at: 1870 3842 1870 Size: 1972 4545 XmnI GAAnn'nnTTC Cuts at: 4646 4646 Size: 6517 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4325 276 Size: 53 83 186 936 2791 2468 Fragments arranged by size: 2791 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI