HUGE

Gene/Protein Characteristic Table for KIAA1904

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00946
Accession No. :	AB067491
Description :	Leucine-rich repeat and fibronectin type-III domain-containing protein 6 precursor.
HUGO Gene Name :	extracellular leucine-rich repeat and fibronectin type III domain containing 2 (ELFN2)
Clone Name :	af00058 [Vector Info]
Flexi ORF Clone :	pF1KSDA1904
Source :	Human brain (amygdala)

Features of the cloned DNA sequence	Description

Length: 7579 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 5111 bp

Genome contig ID gi89161203r_35993947
PolyA signal sequence
(AATAAA,-22)
+----*----+----*----+----*----+----
TTTCTATATTTGCAATAAATTTCTTATTTAAAGCT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ACGGGGCCATGGTCTATGCTGTATTCCTCCCACATTGCACTGCCATGACG

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 22 r 36093947 36101525 1 99.0 Perfect prediction

Features of the protein sequence	Description

Length: 821 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAG10177	100.0	leucine-rich re...	synthetic construct
Q5R3F8	99.8	Leucine-rich re...	Homo sapiens
XP_001086834	98.9	similar to slit...	Macaca mulatta
XP_001500931	97.0	extracellular l...	Equus caballus
Q68FM6	95.4	Leucine-rich re...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB011537	4.1e-09	26.0	KIAA0813
AB040898	4.1e-09	27.1	KIAA1465
AB011538	1.2e-08	33.3	KIAA0814
AB018349	3.4e-08	31.4	KIAA0806
D86983	1.8e-07	26.9	KIAA0230

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR001611 106 119 PR00019 Leucine-rich repeat

IPR001611 151 164 PR00019 Leucine-rich repeat

HMMPfam IPR001611 81 103 PF00560 Leucine-rich repeat

IPR001611 105 127 PF00560 Leucine-rich repeat

IPR001611 153 175 PF00560 Leucine-rich repeat

HMMSmart IPR003591 79 102 SM00369 Leucine-rich repeat

IPR003591 104 126 SM00369 Leucine-rich repeat

IPR003591 127 150 SM00369 Leucine-rich repeat

IPR003591 151 174 SM00369 Leucine-rich repeat

ProfileScan IPR001899 389 430 PS50847 Surface protein from Gram-positive cocci

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001611	106	119	PR00019	Leucine-rich repeat
IPR001611	151	164	PR00019	Leucine-rich repeat
HMMPfam	IPR001611	81	103	PF00560	Leucine-rich repeat
IPR001611	105	127	PF00560	Leucine-rich repeat
IPR001611	153	175	PF00560	Leucine-rich repeat
HMMSmart	IPR003591	79	102	SM00369	Leucine-rich repeat
IPR003591	104	126	SM00369	Leucine-rich repeat
IPR003591	127	150	SM00369	Leucine-rich repeat
IPR003591	151	174	SM00369	Leucine-rich repeat
ProfileScan	IPR001899	389	430	PS50847	Surface protein from Gram-positive cocci

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 3 LRLGLCAAALLCVCRPGAVRADC 25 PRIMARY 23

2 397 HYIMTILGCLFGMVIVLGAVYYC 419 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	3	LRLGLCAAALLCVCRPGAVRADC	25	PRIMARY	23
2	397	HYIMTILGCLFGMVIVLGAVYYC	419	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: ACGCCTCTACTAGCAGCTTTG

Primer_r
: ATGGCACTAGCTTTCCTGGTC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 22

Experimental conditions

Panel name	: genbank
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	5111 bp
Genome contig ID	gi89161203r_35993947
PolyA signal sequence (AATAAA,-22)	+--------+--------+----*----+---- TTTCTATATTTGCAATAAATTTCTTATTTAAAGCT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACGGGGCCATGGTCTATGCTGTATTCCTCCCACATTGCACTGCCATGACG

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	22	r	36093947	36101525	1	99.0	Perfect prediction

Primer_f	: ACGCCTCTACTAGCAGCTTTG
Primer_r	: ATGGCACTAGCTTTCCTGGTC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1904

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information