##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AscI AseI AsnI Asp700I
Asp718I AvrII BamHI BclI BfrI
BlnI BsaWI BseAI BsiWI BsmI
BspEI BssHII Bst1107I BstBI BstZ17I
CpoI DraIII DrdI EcoRI EcoRV
EcoT22I FbaI FseI HindIII KpnI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI SapI SfiI
SfuI SnaBI SpeI SspI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 7561 AAATTT [GA]^AATT[CT]
Aor51HI 2317 AGCGCT AGC^GCT
ApoI 7561 AAATTT [GA]^AATT[CT]
BbrPI 3361 CACGTG CAC^GTG
BciVI 4519 GGATAC GTATCC(6/5)
BsaBI 968 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 1659 ATCGAT AT^CGAT
BspLU11I 4116 ACATGT A^CATGT
BssSI 978 CTCGTG CACGAG(-5/-1)
BstEII 3842 GGTAACC G^GT[ACGT]ACC
BstPI 3842 GGTAACC G^GT[ACGT]ACC
ClaI 1659 ATCGAT AT^CGAT
Eco47III 2317 AGCGCT AGC^GCT
EcoO65I 3842 GGTAACC G^GT[ACGT]ACC
MamI 968 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 1575 CTCGAG C^TCGAG
PmaCI 3361 CACGTG CAC^GTG
PmlI 3361 CACGTG CAC^GTG
SacI 1829 GAGCTC GAGCT^C
SbfI 317 CCTGCAGG CCTGCA^GG
ScaI 2284 AGTACT AGT^ACT
SexAI 7297 ACCTGGT A^CC[AT]GGT
SgrAI 1529 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 317 CCTGCAGG CCTGCA^GG
XhoI 1575 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 1008 GACGTC GACGT^C
3410 GACGTC
AflIII 103 ACGTGT A^C[GA][CT]GT
4116 ACATGT
AhdI 4182 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5025 GACTGTCTGTC
AspEI 4182 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5025 GACTGTCTGTC
AspI 1146 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1629 GACAAGGTC
BsaAI 102 TACGTG [CT]AC^GT[GA]
3361 CACGTG
BsmBI 3412 CGTCTC CGTCTC(1/5)
6359 GAGACG
Bsp1407I 970 TGTACA T^GTACA
2356 TGTACA
BspHI 1012 TCATGA T^CATGA
1198 TCATGA
BsrGI 970 TGTACA T^GTACA
2356 TGTACA
DraI 7481 TTTAAA TTT^AAA
7571 TTTAAA
Eam1105I 4182 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5025 GACTGTCTGTC
HpaI 4433 GTTAAC GTT^AAC
7426 GTTAAC
KspI 657 CCGCGG CCGC^GG
6024 CCGCGG
NcoI 3547 CCATGG C^CATGG
3838 CCATGG
PflFI 1146 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1629 GACAAGGTC
PshAI 3995 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5448 GACTCGGGTC
RcaI 1012 TCATGA T^CATGA
1198 TCATGA
SacII 657 CCGCGG CCGC^GG
6024 CCGCGG
SphI 367 GCATGC GCATG^C
1273 GCATGC
SspBI 970 TGTACA T^GTACA
2356 TGTACA
Tth111I 1146 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1629 GACAAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII AviII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI