HUGE

Gene/Protein Characteristic Table for KIAA1851

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00287
Accession No. :	AB058754
Description :	Amphoterin-induced protein 3 precursor.
HUGO Gene Name :	GDP-mannose pyrophosphorylase B (GMPPB)
Clone Name :	hg04492 [Vector Info]
Flexi ORF Clone :	pF1KA1851
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 6077 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
YES

Integrity of 3' end

Length of 3'UTR 1107 bp

Genome contig ID gi89161205r_49629281
PolyA signal sequence
(AATAAA,-28)
+----*----+----*----+----*----+----
AGAGTTTAATAAAGGTAGCACATGCTTATTGCTAG
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AGCCTTTGCAGACAATGGCGGTCCTCCGTGTGTTCAGCTCTCCTGGCGGC

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 3 r 49729281 49736353 10 99.1 Perfect prediction

Features of the protein sequence	Description

Length: 523 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q86WK7	0	100.0	Amphoterin-indu...	Homo sapiens
AAI01366	0	99.8	Adhesion molecu...	Homo sapiens
XP_001107400	2.4e-212	96.4	similar to amph...	Macaca mulatta
XP_850642	1.5e-182	82.5	similar to GDP-...	Canis lupus fam...
Q80ZD5	5e-170	78.7	Amphoterin-indu...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB032989	5.4e-25	37.3	KIAA1163
AB011538	7.4e-14	29.0	KIAA0814
AB011537	1.9e-12	31.9	KIAA0813
AB007876	8.4e-10	33.0	KIAA0416
D86983	1.4e-09	27.1	KIAA0230

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR001611 130 143 PR00019 Leucine-rich repeat

IPR001611 201 214 PR00019 Leucine-rich repeat

HMMPfam IPR000372 52 79 PF01462 Leucine-rich repeat

IPR001611 105 127 PF00560 Leucine-rich repeat

IPR001611 129 151 PF00560 Leucine-rich repeat

IPR001611 153 175 PF00560 Leucine-rich repeat

IPR001611 177 199 PF00560 Leucine-rich repeat

IPR001611 203 225 PF00560 Leucine-rich repeat

HMMSmart IPR003591 78 102 SM00369 Leucine-rich repeat

IPR003591 103 126 SM00369 Leucine-rich repeat

IPR003591 128 150 SM00369 Leucine-rich repeat

IPR003591 151 174 SM00369 Leucine-rich repeat

IPR003591 175 195 SM00369 Leucine-rich repeat

IPR003591 202 225 SM00369 Leucine-rich repeat

IPR003599 304 391 SM00409 Immunoglobulin subtype

IPR003598 310 378 SM00408 Immunoglobulin subtype 2

ProfileScan IPR007110 296 389 PS50835 Immunoglobulin-like

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001611	130	143	PR00019	Leucine-rich repeat
IPR001611	201	214	PR00019	Leucine-rich repeat
HMMPfam	IPR000372	52	79	PF01462	Leucine-rich repeat
IPR001611	105	127	PF00560	Leucine-rich repeat
IPR001611	129	151	PF00560	Leucine-rich repeat
IPR001611	153	175	PF00560	Leucine-rich repeat
IPR001611	177	199	PF00560	Leucine-rich repeat
IPR001611	203	225	PF00560	Leucine-rich repeat
HMMSmart	IPR003591	78	102	SM00369	Leucine-rich repeat
IPR003591	103	126	SM00369	Leucine-rich repeat
IPR003591	128	150	SM00369	Leucine-rich repeat
IPR003591	151	174	SM00369	Leucine-rich repeat
IPR003591	175	195	SM00369	Leucine-rich repeat
IPR003591	202	225	SM00369	Leucine-rich repeat
IPR003599	304	391	SM00409	Immunoglobulin subtype
IPR003598	310	378	SM00408	Immunoglobulin subtype 2
ProfileScan	IPR007110	296	389	PS50835	Immunoglobulin-like

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 10 CSVLSLVVAAMTWLVLLGTLLCM 32 PRIMARY 23

2 403 GFTTLLGCAVGLVLVLLYLFAPP 425 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	10	CSVLSLVVAAMTWLVLLGTLLCM	32	PRIMARY	23
2	403	GFTTLLGCAVGLVLVLLYLFAPP	425	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: GCTTCACAACTTCACGCAGAG

Primer_r
: GATCCCCTTCACTGTGTCTTG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 3

Experimental conditions

Panel name	: unigene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	YES

Length of 3'UTR	1107 bp
Genome contig ID	gi89161205r_49629281
PolyA signal sequence (AATAAA,-28)	+--------+--------+----*----+---- AGAGTTTAATAAAGGTAGCACATGCTTATTGCTAG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AGCCTTTGCAGACAATGGCGGTCCTCCGTGTGTTCAGCTCTCCTGGCGGC

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	3	r	49729281	49736353	10	99.1	Perfect prediction

Primer_f	: GCTTCACAACTTCACGCAGAG
Primer_r	: GATCCCCTTCACTGTGTCTTG
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1851

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information