HUGE

Gene/Protein Characteristic Table for KIAA1531

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK01174
Accession No. :	AB040964
Description :	Probable G-protein coupled receptor 124 precursor.
HUGO Gene Name :	G protein-coupled receptor 124 (GPR124)
Clone Name :	ph00937 [Vector Info]
Flexi ORF Clone :	pF1KA1531
Source :	Human brain (hippocampus)
Note :	We replaced hj08219, former representative clones for KIAA1531 with ph00937. (2002/5/10)

Features of the cloned DNA sequence	Description

Length: 5350 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 1621 bp

Genome contig ID gi51511724f_37673582
PolyA signal sequence
(ATTAAA,-32)
+----*----+----*----+----*----+----
TATATTAAAATTTTGCAAAGCCCTTTGAGCTACTG
Flanking genome sequence
(147072 - 147121)
----+----*----+----*----+----*----+----*----+----*
CCTTAGTCTACCCACTGTCCTTTTGTTATGAGGTAGAGGATCTCATGACA

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 8 f 37773582 37820652 16 99.5 Perfect prediction

Features of the protein sequence	Description

Length: 1206 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAG11335	0	100.0	G-protein coupl...	synthetic construct
EAW63357	0	99.9	G protein-coupl...	Homo sapiens
AAI46775	0	100.0	GPR124 protein ...	Homo sapiens
BAB84925	2.2e-195	83.2	FLJ00170 protei...	Homo sapiens
AAL11992	2.2e-195	83.6	tumor endotheli...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB058731	4.9e-10	39.2	KIAA1828
D86983	3e-06	28.4	KIAA0230
AB011538	1.2e-05	33.0	KIAA0814
AB011537	1.5e-05	27.1	KIAA0813
AB040898	7.9e-05	31.5	KIAA1465

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR001611 195 208 PR00019 Leucine-rich repeat

IPR001611 216 229 PR00019 Leucine-rich repeat

HMMPfam IPR001611 170 192 PF00560 Leucine-rich repeat

IPR001611 194 216 PF00560 Leucine-rich repeat

IPR001611 218 240 PF00560 Leucine-rich repeat

IPR001611 242 264 PF00560 Leucine-rich repeat

IPR001879 452 506 PF02793 Hormone receptor

HMMSmart IPR003591 167 191 SM00369 Leucine-rich repeat

IPR003591 192 215 SM00369 Leucine-rich repeat

IPR003591 216 239 SM00369 Leucine-rich repeat

IPR003591 240 263 SM00369 Leucine-rich repeat

IPR000483 275 325 SM00082 Cysteine-rich flanking region

IPR003599 338 431 SM00409 Immunoglobulin subtype

ProfileScan IPR007110 345 429 PS50835 Immunoglobulin-like

IPR001879 412 506 PS50227 Hormone receptor

IPR000832 637 812 PS50261 GPCR

ScanRegExp IPR000832 929 944 PS00650 GPCR

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001611	195	208	PR00019	Leucine-rich repeat
IPR001611	216	229	PR00019	Leucine-rich repeat
HMMPfam	IPR001611	170	192	PF00560	Leucine-rich repeat
IPR001611	194	216	PF00560	Leucine-rich repeat
IPR001611	218	240	PF00560	Leucine-rich repeat
IPR001611	242	264	PF00560	Leucine-rich repeat
IPR001879	452	506	PF02793	Hormone receptor
HMMSmart	IPR003591	167	191	SM00369	Leucine-rich repeat
IPR003591	192	215	SM00369	Leucine-rich repeat
IPR003591	216	239	SM00369	Leucine-rich repeat
IPR003591	240	263	SM00369	Leucine-rich repeat
IPR000483	275	325	SM00082	Cysteine-rich flanking region
IPR003599	338	431	SM00409	Immunoglobulin subtype
ProfileScan	IPR007110	345	429	PS50835	Immunoglobulin-like
IPR001879	412	506	PS50227	Hormone receptor
IPR000832	637	812	PS50261	GPCR
ScanRegExp	IPR000832	929	944	PS00650	GPCR

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 637 LHPVVYPCTALLLLCLFATIITY 659 PRIMARY 23

2 674 HMLLNLCFHIAMTSAVFAGGITL 696 SECONDARY 23

3 751 PMLRFYLIAGGIPLIICGITAAV 773 SECONDARY 23

4 796 FYIPVALILLITWIYFLCAGLR 817 PRIMARY 22

5 884 GVQLGALVTTHFLYLAMWACGAL 906 SECONDARY 23

6 916 VVCSCLYGVAASALGLFVFTHHC 938 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	637	LHPVVYPCTALLLLCLFATIITY	659	PRIMARY	23
2	674	HMLLNLCFHIAMTSAVFAGGITL	696	SECONDARY	23
3	751	PMLRFYLIAGGIPLIICGITAAV	773	SECONDARY	23
4	796	FYIPVALILLITWIYFLCAGLR	817	PRIMARY	22
5	884	GVQLGALVTTHFLYLAMWACGAL	906	SECONDARY	23
6	916	VVCSCLYGVAASALGLFVFTHHC	938	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TCATTTGATCTCCAGCTCCAC

Primer_r
: TGGTCCTGTAGTGAAATGCTG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 8

Experimental conditions

Panel name	: unigene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	1621 bp
Genome contig ID	gi51511724f_37673582
PolyA signal sequence (ATTAAA,-32)	+--------+--------+----*----+---- TATATTAAAATTTTGCAAAGCCCTTTGAGCTACTG
Flanking genome sequence (147072 - 147121)	----+--------+--------+--------+--------+----* CCTTAGTCTACCCACTGTCCTTTTGTTATGAGGTAGAGGATCTCATGACA

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	8	f	37773582	37820652	16	99.5	Perfect prediction

Primer_f	: TCATTTGATCTCCAGCTCCAC
Primer_r	: TGGTCCTGTAGTGAAATGCTG
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1531

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information