HUGE

Gene/Protein Characteristic Table for KIAA0175

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK05968
Accession No. :	D79997
Description :	Maternal embryonic leucine zipper kinase.
HUGO Gene Name :	maternal embryonic leucine zipper kinase (MELK)
Clone Name :	ha02337 [Vector Info]
Source :	Myeloblast cell line (KG-1)

Features of the cloned DNA sequence	Description

Length: 2470 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 344 bp

Genome contig ID gi89161216f_36462873
PolyA signal sequence
(AATAAA,-21)
+----*----+----*----+----*----+----
TAATTTCTTTCTGAAATAAAACCATTTGTGAATAT
Flanking genome sequence
(204807 - 204856)
----+----*----+----*----+----*----+----*----+----*
AGCAGGCTTCTCTTTTTTGTATTTGATTTTGATCCAAATAAAACCTCAAA

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 9 f 36562873 36667678 18 100.0 Perfect prediction

Features of the protein sequence	Description

Length: 656 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q14680	100.0	Maternal embryo...	Homo sapiens
BAD96531	99.8	maternal embryo...	Homo sapiens
XP_001168921	99.5	maternal embryo...	Pan troglodytes
XP_001115076	96.5	maternal embryo...	Macaca mulatta
BAF73615	99.8	maternal embryo...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB058763	1.2e-45	42.9	KIAA1860
AB023216	4.2e-42	39.9	KIAA0999
AB018324	1.8e-41	37.9	KIAA0781
D43636	7.8e-40	36.5	KIAA0096
AB011109	2.1e-38	31.0	KIAA0537

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

BlastProDom IPR000719 16 267 PD000001 Protein kinase

HMMPfam IPR000719 16 268 PF00069 Protein kinase

IPR001772 607 656 PF02149 Kinase-associated KA1

HMMSmart IPR001245 16 268 SM00219 Tyrosine protein kinase

IPR002290 16 268 SM00220 Serine/threonine protein kinase

ProfileScan IPR000719 16 268 PS50011 Protein kinase

IPR001772 607 656 PS50032 Kinase-associated KA1

ScanRegExp IPR000719 22 45 PS00107 Protein kinase

IPR008271 133 145 PS00108 Serine/threonine protein kinase

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR000719	16	267	PD000001	Protein kinase
HMMPfam	IPR000719	16	268	PF00069	Protein kinase
IPR001772	607	656	PF02149	Kinase-associated KA1
HMMSmart	IPR001245	16	268	SM00219	Tyrosine protein kinase
IPR002290	16	268	SM00220	Serine/threonine protein kinase
ProfileScan	IPR000719	16	268	PS50011	Protein kinase
IPR001772	607	656	PS50032	Kinase-associated KA1
ScanRegExp	IPR000719	22	45	PS00107	Protein kinase
IPR008271	133	145	PS00108	Serine/threonine protein kinase

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 25 GGFAKVKLACHILTGEMVAIKIM 47 SECONDARY 23

2 195 ADVWSMGILLYVLMCGFLPF 214 PRIMARY 20

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	25	GGFAKVKLACHILTGEMVAIKIM	47	SECONDARY	23
2	195	ADVWSMGILLYVLMCGFLPF	214	PRIMARY	20

Expression profile	Description

: Northern blot Description

Northern blot	Description

RH mapping information	Description

Chromosome No.

: 9

Experimental conditions

Panel name	: Genebridge 4
Primer_f	: GGATGAGTGTGGGTGTGATAC
Primer_r	: TGACAGATGGGCTTGATTTAG
PCR product length	: 173 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	344 bp
Genome contig ID	gi89161216f_36462873
PolyA signal sequence (AATAAA,-21)	+--------+--------+----*----+---- TAATTTCTTTCTGAAATAAAACCATTTGTGAATAT
Flanking genome sequence (204807 - 204856)	----+--------+--------+--------+--------+----* AGCAGGCTTCTCTTTTTTGTATTTGATTTTGATCCAAATAAAACCTCAAA

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	9	f	36562873	36667678	18	100.0	Perfect prediction

Gene/Protein Characteristic Table for KIAA0175

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information