##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflIII      AgeI        Aor51HI     AscI        Asp718I     
AspI        AviII       AvrII       BalI        BamHI       
BbrPI       BbvCI       BcgI        BciVI       BglII       
BlnI        BsaAI       BsaWI       BseAI       BsgI        
BsiWI       BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Cfr10I      ClaI        CpoI        DraIII      
DrdI        DsaI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HpaI        KpnI        
KspI        MaeII       MfeI        MluI        MluNI       
MroI        MscI        MunI        NaeI        NcoI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccII          63  CGCG                 CG^CG
AcyI          101  GGCGCC               G[GA]^CG[CT]C
AhdI         1215  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        537  GTGCAC               G^TGCAC
AocI          127  CCTCAGG              CC^T[ACGT]AGG
ApaI         1827  GGGCCC               GGGCC^C
ApaLI         537  GTGCAC               G^TGCAC
Asp700I        34  GAAAAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1215  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII        1827  GGGCCC               G[GA]GC[CT]^C
BbeI          101  GGCGCC               GGCGC^C
BclI         1100  TGATCA               T^GATCA
BglI         1134  GCCAAGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          107  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      107  GCTCAGC              GC^T[ACGT]AGC
BsaHI         101  GGCGCC               G[GA]^CG[CT]C
BsiEI          29  CGATCG               CG[GA][CT]^CG
BsmI          877  GCATTC               GAATGC(1/-1)
BstUI          63  CGCG                 CG^CG
BstYI        1694  GGATCT               [GA]^GATC[CT]
Bsu36I        127  CCTCAGG              CC^T[ACGT]AGG
CelII         107  GCTCAGC              GC^T[ACGT]AGC
CfoI          102  GCGC                 GCG^C
EaeI           15  CGGCCA               [CT]^GGCC[GA]
Eam1105I     1215  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        127  CCTCAGG              CC^T[ACGT]AGG
EcoT14I       640  CCAAGG               C^C[AT][AT]GG
FbaI         1100  TGATCA               T^GATCA
HaeII         101  GGCGCC               [GA]GCGC^[CT]
HgaI           64  GCGTC                GACGC(5/10)
HhaI          102  GCGC                 GCG^C
Hin1I         101  GGCGCC               G[GA]^CG[CT]C
HinP1I        102  GCGC                 G^CGC
HindIII      1842  AAGCTT               A^AGCTT
KasI          101  GGCGCC               G^GCGCC
MflI         1694  GGATCT               [GA]^GATC[CT]
MspA1I       2055  CAGCGG               C[AC]G^C[GT]G
MvnI           63  CGCG                 CG^CG
NarI          101  GGCGCC               GG^CGCC
PflMI        1351  CCAAGTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         562  GGGACCT              [GA]G^G[AT]CC[CT]
PstI          900  CTGCAG               CTGCA^G
PvuI           29  CGATCG               CGAT^CG
SfaNI        2070  GATGC                GCATC(5/9)
SfoI          101  GGCGCC               GGC^GCC
SmaI          496  CCCGGG               CCC^GGG
StuI          346  AGGCCT               AGG^CCT
StyI          640  CCAAGG               C^C[AT][AT]GG
Van91I       1351  CCAAGTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          257  CCATATCCTTACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1694  GGATCT               [GA]^GATC[CT]
XmaCI         496  CCCGGG               C^CCGGG
XmaI          496  CCCGGG               C^CCGGG
XmnI           34  GAAAAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AcsI          578  AAATTT               [GA]^AATT[CT]
             1622  AAATTC              
AflII        2061  CTTAAG               C^TTAAG
             2247  CTTAAG              
ApoI          578  AAATTT               [GA]^AATT[CT]
             1622  AAATTC              
AseI         1280  ATTAAT               AT^TAAT
             2360  ATTAAT              
AsnI         1280  ATTAAT               AT^TAAT
             2360  ATTAAT              
AspHI         537  GTGCAC               G[AT]GC[AT]^C
             1788  GTGCTC              
AvaI          496  CCCGGG               C^[CT]CG[GA]G
             1144  CTCGGG              
AvaII         563  GGACC                G^G[AT]CC
              908  GGACC               
BanI          101  GGCGCC               G^G[CT][GA]CC
             1675  GGTGCC              
BfrI         2061  CTTAAG               C^TTAAG
             2247  CTTAAG              
BsaBI         591  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              654  GATTACCATC          
BsaI          492  GAGACC               GGTCTC(1/5)
              624  GGTCTC              
BsiHKAI       537  GTGCAC               G[AT]GC[AT]^C
             1788  GTGCTC              
BsmBI          65  CGTCTC               CGTCTC(1/5)
             1822  GAGACG              
BsmFI         222  GGGAC                GGGAC(10/14)
              562  GGGAC               
BsoBI         496  CCCGGG               C^[CT]CG[GA]G
             1144  CTCGGG              
Bsp1407I     1043  TGTACA               T^GTACA
             1937  TGTACA              
BspMI        1415  ACCTGC               ACCTGC(4/8)
              902  GCAGGT              
BsrBI         105  CCGCTC               CCGCTC(-3/-3)
             1679  CCGCTC              
BsrGI        1043  TGTACA               T^GTACA
             1937  TGTACA              
DraI         2204  TTTAAA               TTT^AAA
             2270  TTTAAA              
DraII          81  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              562  GGGACCT             
EarI          486  GAAGAG               CTCTTC(1/4)
             1476  GAAGAG              
EcoO109I       81  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              562  GGGACCT             
FauI            4  GCGGG                CCCGC(4/6)
             1275  GCGGG               
Ksp632I       486  GAAGAG               CTCTTC(1/4)
             1476  GAAGAG              
MamI          591  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              654  GATTACCATC          
NdeI         1710  CATATG               CA^TATG
             2393  CATATG              
NspI          669  ACATGC               [GA]CATG^[CT]
             1925  GCATGT              
PshAI         618  GACTTTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1649  GACAGGTGTC          
PshBI        1280  ATTAAT               AT^TAAT
             2360  ATTAAT              
SfcI          663  CTACAG               C^T[GA][CT]AG
              900  CTGCAG              
SspBI        1043  TGTACA               T^GTACA
             1937  TGTACA              
SspI          196  AATATT               AAT^ATT
              413  AATATT              
TaqI           32  TCGA                 T^CGA
             1015  TCGA                
TthHB8I        32  TCGA                 T^CGA
             1015  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HincII      HindII      HinfI       HpaII       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScaI        ScrFI       SmlI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI