HUGE

Gene/Protein Characteristic Table for KIAA0135

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00421
Accession No. :	D50925
Description :	PAS domain-containing serine/threonine-protein kinase.
HUGO Gene Name :	PAS domain containing serine/threonine kinase (PASK)
Clone Name :	ha01203s1 [Vector Info]
Flexi ORF Clone :	pF1KSDA0135
Source :	Myeloblast cell line (KG-1)
Note :	We replaced ha01203, former representative clones for KIAA0135 with ha01203s1. (2002/5/10)

Features of the cloned DNA sequence	Description

Length: 4326 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 269 bp

Genome contig ID gi89161199r_241594385
PolyA signal sequence
(ATTAAA,-22)
+----*----+----*----+----*----+----
ACTGTTTTATATTATTAAAGGGCTTTTAATTTGTG
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AACTTCTGAAGGCATGAGTGTTTTCTCTTTCTACTTTTGTATATGTGCAT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 2 r 241694385 241737523 18 99.5 Perfect prediction

Features of the protein sequence	Description

Length: 1351 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAG09616	100.0	PAS domain-cont...	synthetic construct
EAW71241	99.6	PAS domain cont...	Homo sapiens
EAW71236	99.5	PAS domain cont...	Homo sapiens
AAK69752	99.1	PAS-kinase [Hom...	Homo sapiens
Q96RG2	99.1	PAS domain-cont...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB058763	3.9e-18	31.9	KIAA1860
D43636	3.9e-17	32.7	KIAA0096
AB058714	5.2e-17	35.8	KIAA1811
AB011109	7.1e-17	32.6	KIAA0537
AB018324	1.8e-16	29.5	KIAA0781

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

BlastProDom IPR000719 1025 1278 PD000001 Protein kinase

HMMPfam IPR013767 154 256 PF00989 PAS fold

IPR000719 1020 1279 PF00069 Protein kinase

HMMSmart IPR000014 142 209 SM00091 PAS

IPR000014 356 423 SM00091 PAS

IPR001245 1020 1277 SM00219 Tyrosine protein kinase

IPR002290 1020 1279 SM00220 Serine/threonine protein kinase

HMMTigr IPR000014 138 265 TIGR00229 PAS

ProfileScan IPR000014 155 211 PS50112 PAS

IPR000719 1020 1279 PS50011 Protein kinase

ScanRegExp IPR000719 1026 1053 PS00107 Protein kinase

IPR008271 1152 1164 PS00108 Serine/threonine protein kinase

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR000719	1025	1278	PD000001	Protein kinase
HMMPfam	IPR013767	154	256	PF00989	PAS fold
IPR000719	1020	1279	PF00069	Protein kinase
HMMSmart	IPR000014	142	209	SM00091	PAS
IPR000014	356	423	SM00091	PAS
IPR001245	1020	1277	SM00219	Tyrosine protein kinase
IPR002290	1020	1279	SM00220	Serine/threonine protein kinase
HMMTigr	IPR000014	138	265	TIGR00229	PAS
ProfileScan	IPR000014	155	211	PS50112	PAS
IPR000719	1020	1279	PS50011	Protein kinase
ScanRegExp	IPR000719	1026	1053	PS00107	Protein kinase
IPR008271	1152	1164	PS00108	Serine/threonine protein kinase

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 358 ASVWVFCTISGLITLLPDGTIHG 380 PRIMARY 23

2 402 ITFLIPGFYSYMDLAYNSSLQLP 424 SECONDARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	358	ASVWVFCTISGLITLLPDGTIHG	380	PRIMARY	23
2	402	ITFLIPGFYSYMDLAYNSSLQLP	424	SECONDARY	23

Expression profile	Description

: Northern blot Description

Northern blot	Description

RH mapping information	Description

Chromosome No.

: 2

Experimental conditions

Panel name	: Stanford G3
Primer_f	: TTCCTGCTTTTCTCCACTTGG
Primer_r	: CATCACTGTCTTCTGTTCTGG
PCR product length	: 146 bp
PCR conditions	: 95 °C15 sec62 °C120 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	269 bp
Genome contig ID	gi89161199r_241594385
PolyA signal sequence (ATTAAA,-22)	+--------+--------+----*----+---- ACTGTTTTATATTATTAAAGGGCTTTTAATTTGTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AACTTCTGAAGGCATGAGTGTTTTCTCTTTCTACTTTTGTATATGTGCAT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	2	r	241694385	241737523	18	99.5	Perfect prediction

Gene/Protein Characteristic Table for KIAA0135

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information