Gene/Protein Characteristic Table for KIAA0666
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Product ID ORK00599
Accession No AB014566
Description dishevelled associated activator of morphogenesis 1, transcript variant 1
Clone name hk02075
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4153 bp)
Predicted protein sequence (1085 aa)
Flexi ORF Clone FXC00599
Source Human adult brain
Rouge ID mKIAA0666 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 4153 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Features of the protein sequence
Description

Length: 1085 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q9Y4D1 0 100.0 Disheveled-asso...
Homo sapiens
XP_001093607 0 99.3 similar to dish...
Macaca mulatta
XP_852088 0 98.6 similar to dish...
Canis lupus fam...
XP_001497343 0 98.5 dishevelled ass...
Equus caballus
XP_865057 0 97.7 similar to dish...
Canis lupus fam...
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB002379 1.3e-66 67.7 KIAA0381
AB095934 4.8e-14 26.8 KIAA2014
AB067489 1.3e-12 27.1 KIAA1902
AB051514 3e-09 26.9 KIAA1727
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR010473 52 240 PF06371 Diaphanous GTPase-binding
IPR010472 242 448 PF06367 Diaphanous FH3
IPR015425 608 991 PF02181 Actin-binding FH2
HMMSmart IPR003104 607 1078 SM00498 Actin-binding FH2 and DRF autoregulatory
ProfileScan IPR014768 52 427 PS51232 GTPase-binding/formin homology 3
IPR014767 1034 1065 PS51231 Diaphanous autoregulatory
Expression profile
Description

RT-PCR
RT-PCR-ELISA
Experimental conditions
Primer_f CACATCAGTAATAGGACCAGC
Primer_r GCACTTGGCAGAGATAACTTG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 14
Experimental conditions
Panel name GeneBridge 4
Primer_f CACATCAGTAATAGGACCAGC
Primer_r GCACTTGGCAGAGATAACTTG
PCR product length 184 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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