Gene/Protein Characteristic Table for KIAA0032
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Product ID ORK00376
Accession No D25215
Description HECT and RLD domain containing E3 ubiquitin protein ligase 3, transcript variant 1
Clone name ha01920
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (4894 bp)
Predicted protein sequence (1054 aa)
Flexi ORF Clone FXC00376
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0032 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 4894 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 1575 bp
Genome contig ID gi89161207f_89632670
PolyA signal sequence
(AATAAA,-22)
+----*----+----*----+----*----+----
TTCAATATTATGCAATAAATTTGGTGTTTTAACTT
Flanking genome sequence
(216041 - 216090)
----+----*----+----*----+----*----+----*----+----*
AAAACTATTTCTTATTGTACTTGCAGAATGGATAGCTTGCTTTTAGTAGA

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 4 f 89732670 89848709 26 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 1054 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q15034 0 100.0 Probable E3 ubi...
Homo sapiens
XP_001100859 0 99.7 hect domain and...
Macaca mulatta
XP_001496703 0 98.5 similar to Prob...
Equus caballus
XP_535653 0 98.6 similar to HECT...
Canis lupus fam...
AAI33342 0 97.9 HERC3 protein [...
Bos taurus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB046813 5e-217 56.5 KIAA1593
AB037722 2e-35 31.2 KIAA1301
AB007899 2e-35 30.9 KIAA0439
D42055 3.7e-34 31.1 KIAA0093
AB002320 1.8e-32 29.8 KIAA0322
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR000408 4 20 PR00633 Regulator of chromosome condensation
IPR000408 40 53 PR00633 Regulator of chromosome condensation
IPR000408 59 75 PR00633 Regulator of chromosome condensation
IPR000408 107 123 PR00633 Regulator of chromosome condensation
IPR000408 146 162 PR00633 Regulator of chromosome condensation
IPR000408 162 176 PR00633 Regulator of chromosome condensation
IPR000408 254 272 PR00633 Regulator of chromosome condensation
IPR000408 313 334 PR00633 Regulator of chromosome condensation
HMMPfam IPR000408 56 103 PF00415 Regulator of chromosome condensation
IPR000408 106 156 PF00415 Regulator of chromosome condensation
IPR000408 159 209 PF00415 Regulator of chromosome condensation
IPR000408 212 261 PF00415 Regulator of chromosome condensation
IPR000569 756 1054 PF00632 HECT
HMMSmart IPR000569 725 1054 SM00119 HECT
ProfileScan IPR000408 3 56 PS50012 Regulator of chromosome condensation
IPR000408 57 106 PS50012 Regulator of chromosome condensation
IPR000408 107 159 PS50012 Regulator of chromosome condensation
IPR000408 160 212 PS50012 Regulator of chromosome condensation
IPR000408 213 264 PS50012 Regulator of chromosome condensation
IPR000408 265 316 PS50012 Regulator of chromosome condensation
IPR000408 318 384 PS50012 Regulator of chromosome condensation
IPR000569 727 1054 PS50237 HECT
ScanRegExp IPR000408 43 53 PS00626 Regulator of chromosome condensation
IPR000408 93 103 PS00626 Regulator of chromosome condensation
IPR000408 251 261 PS00626 Regulator of chromosome condensation
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 4
Experimental conditions
Panel name Genebridge 4
Primer_f CAACCTTCCTCATCATGG
Primer_r CTACAATCCTTCATCAAG
PCR product length 156 bp
PCR conditions 95 °C15 sec53 °C60 sec30 cycles
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