Gene/Protein Characteristic Table for KIAA1301
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Product ID ORK00211
Accession No AB037722
Description HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2, transcript variant 1
Clone name fg06833
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (6926 bp)
Predicted protein sequence (1581 aa)
Source Human fetal brain
Rouge ID mKIAA1301 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 6926 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 2024 bp
Genome contig ID gi89161199r_196672222
PolyA signal sequence
(AATACA,-25)
+----*----+----*----+----*----+----
ATTGATCATGAATACAAGTATATATTTAATTTTGC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AAACTTTTCACTTGTTTGTATTCTTTTCATTATAAGGAAAAGTAGTATAA

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 2 r 196772222 197165580 29 99.5 Perfect prediction
Features of the protein sequence
Description

Length: 1581 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q9P2P5 0 100.0 E3 ubiquitin-pr...
Homo sapiens
XP_516001 0 99.6 HECT, C2 and WW...
Pan troglodytes
XP_001917812 0 96.6 HECT, C2 and WW...
Equus caballus
Q6I6G8 0 94.7 E3 ubiquitin-pr...
Mus musculus
EDL99066 0 94.3 similar to HECT...
Rattus norvegicus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB002320 7.4e-132 57.0 KIAA0322
AB007899 2.3e-49 36.0 KIAA0439
D42055 1.2e-43 36.8 KIAA0093
AB046845 3.6e-39 45.9 KIAA1625
AB002310 6.9e-32 34.5 KIAA0312
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR000008 196 291 PF00168 C2 calcium-dependent membrane targeting
IPR001202 818 847 PF00397 WW/Rsp5/WWP
IPR001202 996 1025 PF00397 WW/Rsp5/WWP
IPR000569 1276 1581 PF00632 HECT
HMMSmart IPR000008 195 306 SM00239 C2 calcium-dependent membrane targeting
IPR001202 817 849 SM00456 WW/Rsp5/WWP
IPR001202 995 1027 SM00456 WW/Rsp5/WWP
IPR000569 1244 1581 SM00119 HECT
ProfileScan IPR000008 195 291 PS50004 C2 calcium-dependent membrane targeting
IPR001202 816 849 PS50020 WW/Rsp5/WWP
IPR001202 994 1027 PS50020 WW/Rsp5/WWP
IPR000569 1246 1581 PS50237 HECT
ScanRegExp IPR001202 822 847 PS01159 WW/Rsp5/WWP
IPR001202 1000 1025 PS01159 WW/Rsp5/WWP
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f GACATGGTTCCAGTGGCTTAC
Primer_r AACATAACAAGGTCTGCATCG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 2
Experimental conditions
Panel name GeneBridge 4
Primer_f AGACACAGGGACTTCCGATGC
Primer_r TGGTCACACTCTCATTGCAGG
PCR product length 212 bp
PCR conditions 95 °C15 sec66 °C60 sec30 cycles
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