Gene/Protein Characteristic Table for KIAA0778
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Product ID ORK00634
Accession No AB018321
Description ATPase, Na+/K+ transporting, alpha 2 polypeptide
Clone name fh12074
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5411 bp)
Predicted protein sequence (1049 aa)
Flexi ORF Clone FXC00634
Source Human fetal brain
Rouge ID mKIAA0778 by Kazusa Mouse cDNA Project
Note We replaced hk05292, former representative clones for KIAA0778 with fh12074. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 5411 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 2259 bp
Genome contig ID gi89161185f_158252187
PolyA signal sequence
(AATAAA,-22)
+----*----+----*----+----*----+----
TGTACTTGAAAATAATAAAGTGGCATTTCTTTAAG
Flanking genome sequence
(127811 - 127860)
----+----*----+----*----+----*----+----*----+----*
AAAAAAAAAGAAATCTCTTCCATAATTCAGATTTCTACACTTTATACTTG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 1 f 158352187 158379996 23 99.5 Perfect prediction
Features of the protein sequence
Description

Length: 1049 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_001115318 0 99.7 Na+/K+ -ATPase ...
Macaca mulatta
P50993 0 100.0 Sodium/potassiu...
Homo sapiens
BAG36951 0 99.9 unnamed protein...
Homo sapiens
Q5RCD8 0 99.5 Sodium/potassiu...
Pongo abelii
XP_001929066 0 99.1 Na,K-ATPase alp...
Sus scrofa
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB037768 6.1e-26 32.2 KIAA1347
AB014603 1.1e-25 32.8 KIAA0703
AB058728 0.00012 22.9 KIAA1825
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR006069 130 144 PR00121 ATPase
IPR006069 157 177 PR00121 ATPase
IPR001757 241 255 PR00119 ATPase
IPR006069 321 343 PR00121 ATPase
IPR006069 394 415 PR00121 ATPase
IPR001757 401 415 PR00119 ATPase
IPR006069 531 549 PR00121 ATPase
IPR001757 613 624 PR00119 ATPase
IPR001757 635 645 PR00119 ATPase
IPR001757 741 760 PR00119 ATPase
IPR001757 765 777 PR00119 ATPase
IPR006069 813 834 PR00121 ATPase
IPR006069 880 900 PR00121 ATPase
IPR006069 942 962 PR00121 ATPase
IPR006069 976 1000 PR00121 ATPase
HMMPfam IPR004014 60 143 PF00690 ATPase
IPR008250 162 393 PF00122 E1-E2 ATPase-associated region
IPR005834 397 764 PF00702 Haloacid dehalogenase-like hydrolase
IPR006068 860 1038 PF00689 ATPase
HMMTigr IPR005775 54 1049 TIGR01106 ATPase
IPR001757 162 254 TIGR01494 ATPase
IPR001757 306 431 TIGR01494 ATPase
IPR001757 610 660 TIGR01494 ATPase
IPR001757 715 834 TIGR01494 ATPase
ScanRegExp IPR001757 403 409 PS00154 ATPase

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 2 RLSQTGWCGLGILLVTSPAKVPQ 24 SECONDARY 23
2 125 GGFSILLWIGAILCFLAYGIQAA 147 PRIMARY 23
3 157 LYLGVVLAAVVIVTGCFSYYQ 177 PRIMARY 21
4 319 IQLITGVAVFLGVSFFVLSLILG 341 PRIMARY 23
5 351 LIGIIVANVPEGLLATVTVCLTL 373 PRIMARY 23
6 821 IANIPLPLGTVTILCIDLGTDMV 843 SECONDARY 23
7 941 EFTCHTAFFASIVVVQWADLIIC 963 SECONDARY 23
8 981 IFGLLEETALAAFLSYCPGMGVA 1003 SECONDARY 23
9 1009 LKVTWWFCAFPYSLLIFIYDEV 1030 PRIMARY 22
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f TGGAGATTACTAACTGTGGAC
Primer_r GATAATTAGCCCATGCACTCG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 1
Experimental conditions
Panel name UniGene
Primer_f -
Primer_r -
PCR product length -
PCR conditions -
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