KIAA1347

Product ID	ORK04213
Accession No	AB037768
Description	ATPase, Ca++ transporting, type 2C, member 1
Clone name	fj00739
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4075 bp) Predicted protein sequence (918 aa)
Source	Human fetal brain
Rouge ID	mKIAA1347 by Kazusa Mouse cDNA Project

Length: 4075 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	905 bp
Genome contig ID	gi89161205f_131995616
PolyA signal sequence (TATAAA,-22)	+--------+--------+----*----+---- GTCATCCTCTAAATATAAATCCAAATACCTCAGCT
Flanking genome sequence (208175 - 208224)	----+--------+--------+--------+--------+----* AAGTAATTCTATTTTATTATTTTTACTGTTATGTATGTTTTTAATCATAT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	3	f	132095525	132203789	28	100.0	Perfect prediction

Length: 918 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
P98194	99.9	Calcium-transpo...	Homo sapiens
AAP30009	99.9	secretory pathw...	Homo sapiens
EAW79220	99.9	ATPase, Ca++ tr...	Homo sapiens
XP_001146321	99.8	calcium-transpo...	Pan troglodytes
XP_516748	99.8	calcium-transpo...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB014603	0	66.8	KIAA0703
AB018321	1.4e-27	32.1	KIAA0778
AB058728	1e-07	24.3	KIAA1825

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001757	183	197	PR00119	ATPase
	IPR001757	347	361	PR00119	ATPase
	IPR000695	509	527	PR00120	H+ transporting ATPase
	IPR001757	539	550	PR00119	ATPase
	IPR001757	561	571	PR00119	ATPase
	IPR000695	613	629	PR00120	H+ transporting ATPase
	IPR000695	641	657	PR00120	H+ transporting ATPase
	IPR001757	641	660	PR00119	ATPase
	IPR001757	665	677	PR00119	ATPase
	IPR000695	673	698	PR00120	H+ transporting ATPase
HMMPfam	IPR004014	21	98	PF00690	ATPase
	IPR008250	104	339	PF00122	E1-E2 ATPase-associated region
	IPR005834	343	664	PF00702	Haloacid dehalogenase-like hydrolase
	IPR006068	760	901	PF00689	ATPase
HMMTigr	IPR006413	22	902	TIGR01522	Calcium-transporting P-type ATPase
	IPR001757	104	208	TIGR01494	ATPase
	IPR001757	254	366	TIGR01494	ATPase
	IPR001757	509	586	TIGR01494	ATPase
	IPR001757	613	734	TIGR01494	ATPase
ScanRegExp	IPR001757	349	355	PS00154	ATPase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	75	ISQFKNPLIMLLLASAVISVLMH	97	PRIMARY	23
	2	101	DAVSITVAILIVVTVAFVQEYRS	123	PRIMARY	23
	3	267	QLSFYSFGIIGIIMLVGWLLGKD	289	PRIMARY	23
	4	297	SVSLAVAAIPEGLPIVVTVTLAL	319	PRIMARY	23
	5	772	KNLILKILVSSIIIVCGTLFVFW	794	PRIMARY	23
	6	842	MFCYAVLGSIMGQLLVIYFPPLQ	864	PRIMARY	23
	7	871	SLSILDLLFLLGLTSSVCIVAEI	893	PRIMARY	23

Primer_f	TTGCCTTTATGGGAACACTGG
Primer_r	AACCAGCCAACCAACATGATG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	AGTGGATGGCTGTTAAGTGTG
Primer_r	TCTGCCCTTTGCTCTGGTATG
PCR product length	120(300) bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles