Gene/Protein Characteristic Table for KIAA1825
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Product ID ORK01182
Accession No AB058728
Description ATPase type 13A1
Clone name fj04975
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (3832 bp)
Predicted protein sequence (1203 aa)
Flexi ORF Clone FXC01182
Source Human fetal brain
Rouge ID mKIAA1825 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 3832 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 219 bp
Genome contig ID gi42406306r_19517012
PolyA signal sequence
(AATAAA,-23)
+----*----+----*----+----*----+----
GCACCTTTGGTAAATAAAGCAGCATCTGAGATTTT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AAAAAGCCCTGCCTCCACCTCGTCTGTGCCACACGGTGTTTGCTGGGGGC

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 19 r 19617012 19635473 26 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 1203 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q9HD20 0 100.0 Probable cation...
Homo sapiens
XP_533862 0 96.4 similar to Prob...
Canis lupus fam...
XP_001500954 0 95.8 ATPase type 13A...
Equus caballus
EDL28752 0 95.2 ATPase type 13A...
Mus musculus
Q9EPE9 0 95.1 Probable cation...
Mus musculus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB037768 1.2e-06 24.1 KIAA1347
AB018321 0.0003 23.0 KIAA0778
AB014603 0.0003 22.2 KIAA0703
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR001757 347 361 PR00119 ATPase
IPR001757 530 544 PR00119 ATPase
IPR001757 738 748 PR00119 ATPase
IPR001757 861 880 PR00119 ATPase
HMMPfam IPR008250 301 391 PF00122 E1-E2 ATPase-associated region
IPR005834 526 753 PF00702 Haloacid dehalogenase-like hydrolase
IPR005834 835 884 PF00702 Haloacid dehalogenase-like hydrolase
HMMTigr IPR006544 84 1173 TIGR01657 P-type ATPase of unknown pump specificity (type V)
IPR001757 267 361 TIGR01494 ATPase
IPR001757 444 547 TIGR01494 ATPase
IPR001757 832 884 TIGR01494 ATPase
ScanRegExp IPR001757 532 538 PS00154 ATPase

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 240 RATAPFFVFQVFCVGLWCLDEYW 262 PRIMARY 23
2 264 YSVFTLSMLVAFEASLVQQQMRN 286 SECONDARY 23
3 436 VTANNLETFIFILFLLVFAIAAA 458 PRIMARY 23
4 492 LPIELSLAVNTSLIALAKLYMYC 514 SECONDARY 23
5 983 TLQMFKILALNALILAYSQSVLY 1005 SECONDARY 23
6 1011 FSDFQATLQGLLLAGCFLFISR 1032 SECONDARY 22
7 1048 IFNLYTILTVMLQFFVHFLSLVY 1070 SECONDARY 23
8 1095 PSLVNSTVYIMAMAMQMATFAIN 1117 SECONDARY 23
9 1131 KPLVWSLAVSLLAIIGLLLGSS 1152 PRIMARY 22
10 1166 VEFKLVIAQVLLLDFCLALLADR 1188 PRIMARY 23
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f ATTGTGAAACTGGGGGATGCC
Primer_r ATCTTGAACATCTGTAGCGTG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 19
Experimental conditions
Panel name genbank
Primer_f -
Primer_r -
PCR product length -
PCR conditions -
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