KIAA1939

Product ID	ORK04227
Accession No	AB075819
Description	ATPase, class I, type 8B, member 4
Clone name	ah01164
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector. This cDNA clone ah01164 has an irregular adapter sequence (GTCGACCCAC GCGTCCGCCC ACGCGTCCGC CCACGCGTCC GCCCACGCGT CCGCCCACGC GTCCGCCCAC GCGTCCGCCC ACGCGTCCG) instead of regular one (GTCGACCCAC GCGTCCG).
cDNA sequence	DNA sequence (5782 bp) Predicted protein sequence (1082 aa)
Source	Human brain (amygdala)
Rouge ID	mKIAA1939 by Kazusa Mouse cDNA Project

Length: 5782 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Integrity of 3' end

Length of 3'UTR	1954 bp
Genome contig ID	gi51511731r_47837728
PolyA signal sequence (AGTAAA,-24)	+--------+--------+----*----+---- TGAAAACTTTCAGTAAATGTTTTGGCACTATTGGT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACTTGATGTTTTATCTCTTCTTTATGGGAAATTCATTGGGGAGATTTTTG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	15	r	47937728	48192805	29	99.5	Perfect prediction

Length: 1082 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q8TF62	99.4	Probable phosph...	Homo sapiens
XP_510393	98.9	ATPase class I ...	Pan troglodytes
EAW77381	99.9	ATPase, Class I...	Homo sapiens
XP_001167879	99.3	ATPase class I ...	Pan troglodytes
XP_001499742	93.0	ATPase, class I...	Equus caballus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB032963	0	68.1	KIAA1137
AB028944	1.8e-80	34.8	KIAA1021
AB040920	5.5e-69	35.1	KIAA1487
AB018258	2.1e-65	31.3	KIAA0715
AB011138	5.6e-65	32.1	KIAA0566

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001757	280	294	PR00119	ATPase
FPrintScan	IPR001757	703	722	PR00119	ATPase
HMMPfam	IPR013200	700	720	PF08282	HAD superfamily hydrolase-like
HMMTigr	IPR006539	2	992	TIGR01652	Phospholipid-translocating P-type ATPase
	IPR001757	208	302	TIGR01494	ATPase
	IPR001757	532	581	TIGR01494	ATPase
	IPR001757	676	783	TIGR01494	ATPase
ScanRegExp	IPR001757	282	288	PS00154	ATPase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	163	MNTLVLWIFGFLICLGIILAIGN	185	PRIMARY	23
	2	214	FLTFWSYIIILNTVVPISLYVSV	236	PRIMARY	23
	3	771	FAFTLVHFWFGFFCGFSAQTVYD	793	SECONDARY	23
	4	797	ITLFNIVYTSLPVLAMGIFDQDV	819	SECONDARY	23
	5	845	FFICVLHGIYTSLVLFFIPYGAF	867	PRIMARY	23
	6	881	YQSFAVTMATSLVIVVSVQIALD	903	PRIMARY	23
	7	908	TFINHVFIWGSIAIYFSILFTMH	930	PRIMARY	23
	8	952	LTQKCIWLVILLTTVASVMPVVA	974	PRIMARY	23

Primer_f	GTGCAGATAGCCTTGGATACC
Primer_r	AGAGAATTACAAGCCAGATGC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	genbank
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-