KIAA1487

Product ID	ORK04209
Accession No	AB040920
Description	ATPase, class V, type 10D
Clone name	fj07249
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3992 bp) Predicted protein sequence (650 aa)
Source	Human fetal brain
Rouge ID	mKIAA1487 by Kazusa Mouse cDNA Project

Length: 3992 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	2037 bp
Genome contig ID	gi89161207f_47154940
PolyA signal sequence (AATATA,-17)	+--------+--------+----*----+---- CTTTGCTACAAAAATATTAATATATTTCATTACTG
Flanking genome sequence (135254 - 135303)	----+--------+--------+--------+--------+----* AATGCTAAACTGTATTTCTTTTTAAAATTTGGAAAATTTTTAATTCTGAA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	4	f	47254940	47290192	12	99.4	Perfect prediction

Length: 650 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAB55221	99.8	unnamed protein...	Homo sapiens
EAW93036	99.8	ATPase, Class V...	Homo sapiens
AAI31536	99.7	ATPase, class V...	Homo sapiens
Q9P241	99.5	Probable phosph...	Homo sapiens
XP_001153968	99.2	ATPase, Class V...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB018258	1.6e-134	57.7	KIAA0715
AB011138	2.4e-129	54.4	KIAA0566
AB075819	5e-69	35.1	KIAA1939
AB032963	2.1e-67	35.3	KIAA1137
AB028944	5.4e-62	38.0	KIAA1021

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR013200	274	295	PF08282	HAD superfamily hydrolase-like
HMMTigr	IPR001757	111	161	TIGR01494	ATPase
HMMTigr	IPR001757	251	358	TIGR01494	ATPase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	333	RLSNMILYFFYKNVAYVNLLFWY	355	SECONDARY	23
	2	372	LIFFNLLFTSAPPVIYGVLEKDV	394	SECONDARY	23
	3	418	HTFWITLLDAFYQSLVCFFVPYF	440	SECONDARY	23
	4	456	LNTAALFIVLLHLVIESKSLTWI	478	PRIMARY	23
	5	482	VIIGSILSYFLFAIVFGAMCVT	503	PRIMARY	22
	6	518	MLDPVFYLVCILTTSIALLPRFV	540	PRIMARY	23

Primer_f	CATGTATGCTGGCTTTAGTTG
Primer_r	GAGCATGGAGATTCAGGAAGG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-