KIAA0821

Product ID	ORK00646
Accession No	AB020628
Description	adhesion G protein-coupled receptor L1, transcript variant 1
Clone name	hh02631
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5659 bp) Predicted protein sequence (1566 aa)
HaloTag ORF Clone	FHC00646
Flexi ORF Clone	FXC00646
Source	Human adult brain
Rouge ID	mKIAA0821 by Kazusa Mouse cDNA Project

Length: 5659 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	936 bp
Genome contig ID	gi42406306r_14021763
PolyA signal sequence (AAGAAA,-17)	+--------+--------+----*----+---- TATTTCAACGAAATGGAAAAGAAAAAAAAAAAGAC
Flanking genome sequence (99987 - 99938)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAAGAAAAGAAAAAAAATCTCATCACTTGAAGCCACCG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	19	r	14121750	14178000	23	99.4	Perfect prediction

Length: 1566 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
O94910	100.0	Latrophilin-1; ...	Homo sapiens
AAG27461	99.6	lectomedin-2 [H...	Homo sapiens
EAW84408	99.1	latrophilin 1, ...	Homo sapiens
XP_867067	98.8	similar to latr...	Canis lupus fam...
XP_001789501	98.6	latrophilin 1 i...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB018329	6e-107	63.2	KIAA0786
AB018311	3.3e-59	48.1	KIAA0768
D87469	4.6e-15	27.1	KIAA0279
AB011536	5.3e-14	24.1	KIAA0812
AB011122	2.7e-10	25.6	KIAA0550

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR000922	127	220	PD005612	D-galactoside/L-rhamnose binding SUEL lectin
FPrintScan	IPR003924	153	164	PR01444	Latrophilin receptor
	IPR003924	175	194	PR01444	Latrophilin receptor
	IPR003924	203	223	PR01444	Latrophilin receptor
	IPR003924	316	338	PR01444	Latrophilin receptor
	IPR003924	359	374	PR01444	Latrophilin receptor
	IPR000832	954	978	PR00249	GPCR
	IPR003924	979	994	PR01444	Latrophilin receptor
	IPR000832	1017	1040	PR00249	GPCR
	IPR000832	1097	1122	PR00249	GPCR
	IPR000832	1171	1192	PR00249	GPCR
	IPR003924	1193	1205	PR01444	Latrophilin receptor
HMMPfam	IPR000922	140	220	PF02140	D-galactoside/L-rhamnose binding SUEL lectin
	IPR003112	234	490	PF02191	Olfactomedin-like
	IPR001879	569	629	PF02793	Hormone receptor
	IPR000203	890	937	PF01825	GPS
	IPR000832	949	1196	PF00002	GPCR
	IPR003334	1206	1566	PF02354	Latrophilin
HMMSmart	IPR003112	234	490	SM00284	Olfactomedin-like
	IPR001879	568	633	SM00008	Hormone receptor
	IPR000203	890	942	SM00303	GPS
ProfileScan	IPR000922	132	221	PS50228	D-galactoside/L-rhamnose binding SUEL lectin
	IPR003112	231	490	PS51132	Olfactomedin-like
	IPR001879	569	628	PS50227	Hormone receptor
	IPR000203	891	942	PS50221	GPS
	IPR000832	952	1193	PS50261	GPCR
ScanRegExp	IPR000832	1181	1196	PS00650	GPCR

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	92	TMARLAAVLWNLCVTAVLVTSAT	114	PRIMARY	23
	2	953	SVITWVGIVISLVCLAICISTFC	975	PRIMARY	23
	3	986	TIHKNLCINLFLAELLFLVGID	1007	SECONDARY	22
	4	1021	LLHYFFLAAFSWLCLEGVHLYLL	1043	PRIMARY	23
	5	1059	YLGGYCFPALVVGIAAAIDYR	1079	PRIMARY	21
	6	1097	WSFIGPVSFVIVVNLVFLMVTLH	1119	PRIMARY	23
	7	1139	KSWALGAIALLFLLGLTWAFGLL	1161	PRIMARY	23
	8	1170	MAYLFTTFNAFQGVFIFVFHCAL	1192	SECONDARY	23

Primer_f	TCCTGATGAAGCACAACTCCC
Primer_r	TTTACAGCAATGGGGAAGGAG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	TCCTGATGAAGCACAACTCCC
Primer_r	TTTACAGCAATGGGGAAGGAG
PCR product length	203 bp
PCR conditions	95 °C15 sec64 °C60 sec35 cycles