##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AclI AflII AgeI AhdI AscI
AseI AsnI AspEI AviII BciVI
BfrI BsaI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI Eam1105I
EcoRV EcoT22I FspI HindIII HpaI
KspI MfeI MluI MunI NdeI
NheI NotI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SfuI SgrAI
SnaBI SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 3833 GGTACC G^GTACC
AccIII 274 TCCGGA T^CCGGA
AcsI 3741 GAATTC [GA]^AATT[CT]
Aor51HI 1233 AGCGCT AGC^GCT
ApoI 3741 GAATTC [GA]^AATT[CT]
Asp718I 3833 GGTACC G^GTACC
AvrII 5570 CCTAGG C^CTAGG
BcgI 1495 CGACCCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4146 TGATCA T^GATCA
BglII 2844 AGATCT A^GATCT
BlnI 5570 CCTAGG C^CTAGG
BlpI 3014 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3014 GCTGAGC GC^T[ACGT]AGC
BsaBI 4144 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 274 TCCGGA [AT]^CCGG[AT]
BseAI 274 TCCGGA T^CCGGA
BspEI 274 TCCGGA T^CCGGA
CelII 3014 GCTGAGC GC^T[ACGT]AGC
DraI 5305 TTTAAA TTT^AAA
DrdI 754 GACACACGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4392 CGGCCG C^GGCCG
EclXI 4392 CGGCCG C^GGCCG
Eco47III 1233 AGCGCT AGC^GCT
Eco52I 4392 CGGCCG C^GGCCG
EcoRI 3741 GAATTC G^AATTC
FbaI 4146 TGATCA T^GATCA
FseI 1767 GGCCGGCC GGCCGG^CC
KpnI 3833 GGTACC GGTAC^C
MamI 4144 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 274 TCCGGA T^CCGGA
NruI 1426 TCGCGA TCG^CGA
NspI 1785 GCATGC [GA]CATG^[CT]
PaeR7I 1818 CTCGAG C^TCGAG
PshAI 635 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 2746 GTCGAC G^TCGAC
SbfI 4606 CCTGCAGG CCTGCA^GG
ScaI 3189 AGTACT AGT^ACT
SfiI 2552 GGCCCGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 3151 ACTAGT A^CTAGT
SphI 1785 GCATGC GCATG^C
Sse8387I 4606 CCTGCAGG CCTGCA^GG
SspI 5623 AATATT AAT^ATT
XhoI 1818 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
Asp700I 703 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2683 GAACCACTTC
BalI 2922 TGGCCA TGG^CCA
5467 TGGCCA
BamHI 834 GGATCC G^GATCC
3680 GGATCC
BsiEI 2968 CGACCG CG[GA][CT]^CG
4392 CGGCCG
BsrDI 5613 CATTGC GCAATG(2/0)
2457 GCAATG
DraIII 595 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1622 CACCCAGTG
MluNI 2922 TGGCCA TGG^CCA
5467 TGGCCA
MscI 2922 TGGCCA TGG^CCA
5467 TGGCCA
MseI 3435 TTAA T^TAA
5306 TTAA
SacI 270 GAGCTC GAGCT^C
1727 GAGCTC
SapI 1729 GCTCTTC GCTCTTC(1/4)
3022 GCTCTTC
Tru9I 3435 TTAA T^TAA
5306 TTAA
XmnI 703 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2683 GAACCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI AspHI
AspI AvaI AvaII BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PflFI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI